Encapsulating Quantum Dots in Lipid–PEG Micelles and Subsequent Copper-Free Click Chemistry Bioconjugation

Saeboe, Alexander; Kays, Joshua; Dennis, Allison M.

doi:10.1007/978-1-0716-0463-2_5

Cited by 10 publications

(7 citation statements)

References 32 publications

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“…Following a previously published protocol, QDs were rendered water soluble through encapsulation in lipid-PEG micelles using 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)] (ammonium salt) with 5000 Da PEG (DSPE-PEG5k, Avanti Polar Lipids, Inc.). 50, 51 The water soluble samples largely retained their pre-coating brightness, exhibiting QYs between 11 and 18%, consistent with similarly treated commercial QD705s (Thermo Fisher) ( Table S2) .…”

supporting

confidence: 68%

Extending the Near Infrared Emission Range of Indium Phosphide Quantum Dots for MultiplexedIn VivoImaging

Saeboe

Nikiforov

Toufanian

et al. 2021

Preprint

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This report of the reddest emitting indium phosphide quantum dots (InP QDs) to date demonstrates tunable, near infrared (NIR) photoluminescence and fluorescence multiplexing in the first optical tissue window with a material that avoids toxic constituents. This synthesis overcomes the InP synthesis 'growth bottleneck' and extends the emission peak of InP QDs deeper into the first optical tissue window using an inverted QD heterostructure. The ZnSe/InP/ZnS core/shell/shell structure is designed to produce emission from excitons with heavy holes confined in InP shells wrapped around larger-bandgap ZnSe cores and protected by a second shell of ZnS. The InP QDs exhibit InP shell thickness-dependent tunable emission with peaks ranging from 515 - 845 nm. The high absorptivity of InP leads to effective absorbance and photoexcitation of the QDs with UV, visible, and NIR wavelengths in particles with diameters of eight nanometers or less. These nanoparticles extend the range of tunable direct-bandgap emission from InP-based nanostructures, effectively overcoming a synthetic barrier that has prevented InP-based QDs from reaching their full potential as NIR imaging agents. Multiplexed lymph node imaging in a mouse model shows the potential of the NIR-emitting InP particles for in vivo imaging.

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confidence: 68%

Extending the Near Infrared Emission Range of Indium Phosphide Quantum Dots for MultiplexedIn VivoImaging

Saeboe

Nikiforov

Toufanian

et al. 2021

Preprint

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“…To demonstrate the photothermal effect of the much smaller CFS nanoparticles, we compared the ensemble heating behavior of oxidized CFS-7:1 to gold nanorods (NRs, 67 nm × 10 nm length × width) with 1064 nm LSPR peaks at equivalent mass concentrations (Figure S13). For appropriate comparison to the water-soluble, citrate-capped gold NRs, the bornite NCs were rendered water-soluble by encapsulation in a lipid-PEG micelle. , With no optimization of coatings or size, bornite performed equally well compared to gold nanorods in this simple experiment, with both achieving a 14 °C increase in temperature after 20 min of illumination. The use of smaller particle sizes and bioessential elements may be advantageous in biomedical applications, warranting further studies into the application of bornite CFS.…”

Section: Resultsmentioning

confidence: 99%

“…For appropriate comparison to the water-soluble, citrate-capped gold NRs, the bornite NCs were rendered water-soluble by encapsulation in a lipid-PEG micelle. 41,42 With no optimization of coatings or size, bornite performed equally well compared to gold nanorods in this simple experiment, with both achieving a 14 °C increase in temperature after 20 min of illumination. The use of smaller particle sizes and bioessential elements may be advantageous in biomedical applications, warranting further studies into the application of bornite CFS.…”

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confidence: 86%

Controlled Synthesis and Exploration of Cu_xFeS₄ Bornite Nanocrystals

et al. 2021

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Plasmonic semiconductor nanocrystals (NCs) are a new and exciting class of materials that enable higher control of their localized surface plasmon resonance (LSPR) than metallic counterparts. Additionally, earth-abundant and non-toxic materials such as copper iron sulfides are gaining interest as alternatives to heavy metal-based semiconductor materials. Colloidal bornite (Cu5FeS4) is an interesting but underexplored example of a heavy metal-free plasmonic semiconductor. This report details the hot-injection synthesis of bornite yielding NCs ranging from 2.7 to 6.1 nm in diameter with stoichiometric control of the copper and iron content. The absorbance spectra of bornite NCs with different Cu:Fe ratios change at different rates as the particles oxidize and develop LSPR in the near-infrared region. X-ray photoelectron spectroscopy results indicate that oxidation produces sulfates rather than metal oxides as well as a decrease in the iron content within the NCs. Additionally, increasing iron content leads to decreases in carrier density and effective mass of the carrier, as determined by the Drude model. This controlled synthesis, combined with a further understanding of the relationship between the particle structure and optical properties, will enable the continued development and application of these fascinating heavy metal-free plasmonic semiconductor nanoparticles.

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“…Additionally, the absorbance features provide insight into the encapsulation efficiency and purity; for example, the QD-micelles exhibit a scattering shoulder at wavelengths higher than the band-edge transition 1S transition peak, attributed to the presence of empty micelles (Figure S4). , These were removed from the solution via ultracentrifugation, followed by successive rounds of size-exclusion chromatography to remove smaller QD-micelles (Figure S4). The eluted fractions containing the largest of QD-micelles were determined to have an average hydrodynamic diameter of 99 nm via dynamic light scattering (Figure b).…”

Section: Results and Discussionmentioning

confidence: 99%

Quantum Dot Biomimetic for SARS-CoV-2 to Interrogate Blood–Brain Barrier Damage Relevant to NeuroCOVID Brain Inflammation

Chiang,

Stout,

Yanchik-Slade

et al. 2023

ACS Appl. Nano Mater.

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Despite limited evidence for infection of SARS-CoV-2 in the central nervous system, cognitive impairment is a common complication reported in "recovered" COVID-19 patients. Identification of the origins of these neurological impairments is essential to inform therapeutic designs against them. However, such studies are limited, in part, by the current status of high-fidelity probes to visually investigate the effects of SARS-CoV-2 on the system of blood vessels and nerve cells in the brain, called the neurovascular unit. Here, we report that nanocrystal quantum dot micelles decorated with spike protein (COVID-QDs) are able to interrogate neurological damage due to SARS-CoV-2. In a transwell co-culture model of the neurovascular unit, exposure of brain endothelial cells to COVID-QDs elicited an inflammatory response in neurons and astrocytes without direct interaction with the COVID-QDs. These results provide compelling evidence of an inflammatory response without direct exposure to SARS-CoV-2-like nanoparticles. Additionally, we found that pretreatment with a neuro-protective molecule prevented endothelial cell damage resulting in substantial neurological protection. These results will accelerate studies into the mechanisms by which SARS-CoV-2 mediates neurologic dysfunction.

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Encapsulating Quantum Dots in Lipid–PEG Micelles and Subsequent Copper-Free Click Chemistry Bioconjugation

Cited by 10 publications

References 32 publications

Extending the Near Infrared Emission Range of Indium Phosphide Quantum Dots for MultiplexedIn VivoImaging

Extending the Near Infrared Emission Range of Indium Phosphide Quantum Dots for MultiplexedIn VivoImaging

Controlled Synthesis and Exploration of Cu_xFeS₄ Bornite Nanocrystals

Quantum Dot Biomimetic for SARS-CoV-2 to Interrogate Blood–Brain Barrier Damage Relevant to NeuroCOVID Brain Inflammation

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Encapsulating Quantum Dots in Lipid–PEG Micelles and Subsequent Copper-Free Click Chemistry Bioconjugation

Cited by 10 publications

References 32 publications

Extending the Near Infrared Emission Range of Indium Phosphide Quantum Dots for MultiplexedIn VivoImaging

Extending the Near Infrared Emission Range of Indium Phosphide Quantum Dots for MultiplexedIn VivoImaging

Controlled Synthesis and Exploration of CuxFeS4 Bornite Nanocrystals

Quantum Dot Biomimetic for SARS-CoV-2 to Interrogate Blood–Brain Barrier Damage Relevant to NeuroCOVID Brain Inflammation

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Controlled Synthesis and Exploration of Cu_xFeS₄ Bornite Nanocrystals