Encapsulating genetically modified Saccharomyces cerevisiae cells in a flow-through device towards the detection of diclofenac in wastewater

Schirmer, Christine; Posseckardt, Juliane; Kick, Alfred; Rebatschek, K.; Fichtner, W.; Schuller, Astrid; Rödel, Gerhard; Mertig, Michael

doi:10.1016/j.jbiotec.2018.08.003

Cited by 21 publications

(14 citation statements)

References 35 publications

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“…for use in microfluidic platforms [13]. Very recently a microfluidic flow-through device containing genetically modified yeast cells for the detection of diclofenac in wastewater has been reported [14]. Yeast cells have been used to sense a large variety of analytes including glucose [15,16], copper ions [17][18][19], hormonally active substances [20][21][22], toxic substances [23][24][25] as well asantibiotics [26] or analgesics [27].…”

Section: Whole-cell Sensorsmentioning

confidence: 99%

A fluorescence‐based yeast sensor for monitoring acetic acid

Hahne

Rödel

2021

Engineering in Life Sciences

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Accumulation of acetic acid indicates an imbalance of the process due to a disturbed composition of the microorganisms. Hence, monitoring the acetic acid concentration is an important parameter to control the biogas process. Here, we describe the generation and validation of a fluorescence-based whole cell sensor for the detection of acetic acid based on the yeast Saccharomyces cerevisiae. Acetic acid induces the transcription of a subset of genes. The 5ť-regulatory sequences (5ť URS) of these genes were cloned into a multicopy plasmid to drive the expression of a red fluorescent reporter gene. The 5ť URS of YGP1, encoding a cell wallrelated glycoprotein, led to a 20-fold increase of fluorescence upon addition of 30 mM acetic acid to the media. We show that the system allows estimating the approximate concentration of acetic acid in condensation samples from a biogas plant. To avoid plasmid loss and increase the long-term stability of the sensor, we integrated the reporter construct into the yeast genome and tested the suitability of spores for long-term storage of sensor cells. Lowering the reporter gene's copy number resulted in a significant drop of the fluorescence, which can be compensated by applying a yeast pheromone-based signal amplification system.

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Section: Whole-cell Sensorsmentioning

confidence: 99%

A fluorescence‐based yeast sensor for monitoring acetic acid

Hahne

Rödel

2021

Engineering in Life Sciences

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“…In the last few years, portable yeast biosensors based on fluorescence and colorimetric detection have been developed. Some of them have also been integrated into single‐use and low‐cost microfluidic systems, [ 78,79 ] others have been immobilized in field portable paper devices for point‐of‐need and point‐of‐care applications. [ 80,81 ]…”

Section: Bioluminescence Whole‐cell Biosensorsmentioning

confidence: 99%

Bioluminescence goes portable: recent advances in whole‐cell and cell‐free bioluminescence biosensors

et al. 2020

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Recent advancements in synthetic biology, organic chemistry, and computational models have allowed the application of bioluminescence in several fields, ranging from well established methods for detecting microbial contamination to in vivo imaging to track cancer and stem cells, from cell‐based assays to optogenetics. Moreover, thanks to recent technological progress in miniaturized and sensitive light detectors, such as photodiodes and imaging sensors, it is possible to implement laboratory‐based assays, such as cell‐based and enzymatic assays, into portable analytical devices for point‐of‐care and on‐site applications. This review highlights some recent advances in the development of whole‐cell and cell‐free bioluminescence biosensors with a glance on current challenges and different strategies that have been used to turn bioassays into biosensors with the required analytical performance. Critical issues and unsolved technical problems are also highlighted, to give the reader a taste of this fascinating and challenging field.

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“…[ 15 ] In order to prevent the occurrence of this situation, it is usually impossible to apply these GMO biosensors. [ 16,17 ] Furthermore, the response time of biosensors is prolonged because some analytes are hard to cross over the cell membrane, and some analytes may be toxic to living cells. Therefore, some cell‐based biosensors are not suitable for detecting cytotoxic or impermeable membrane analytes such as organophosphates.…”

Section: Introductionmentioning

confidence: 99%

Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications

Zhang

Guo

2020

Biotechnology Journal

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Synthetic biology has promoted the development of biosensors as tools for detecting trace substances. In the past, biosensors based on synthetic biology have been designed on living cells, but the development of cell biosensors has been greatly limited by defects such as genetically modified organism problem and the obstruction of cell membrane. However, the advent of cell‐free synthetic biology addresses these limitations. Biosensors based on the cell‐free protein synthesis system have the advantages of higher safety, higher sensitivity, and faster response time over cell biosensors, which make cell‐free biosensors have a broader application prospect. This review summarizes the workflow of various cell‐free biosensors, including the identification of analytes and signal output. The detection range of cell‐free biosensors is greatly enlarged by different recognition mechanisms and output methods. In addition, the review also discusses the applications of cell‐free biosensors in environmental monitoring and health diagnosis, as well as existing deficiencies and aspects that should be improved. In the future, through continuous improvement and optimization, the potential of cell‐free biosensors will be stimulated, and their application fields will be expanded.

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Encapsulating genetically modified Saccharomyces cerevisiae cells in a flow-through device towards the detection of diclofenac in wastewater

Cited by 21 publications

References 35 publications

A fluorescence‐based yeast sensor for monitoring acetic acid

A fluorescence‐based yeast sensor for monitoring acetic acid

Bioluminescence goes portable: recent advances in whole‐cell and cell‐free bioluminescence biosensors

Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications

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