Encapsulating Antibiotic and Protein-Stabilized Nanosilver into Sandwich-Structured Electrospun Nanofibrous Scaffolds for MRSA-Infected Wound Treatment

Abstract

With the increasing prevalence of microbial infections, which results in prolonged inflammation and delayed wound healing, the development of effective and safe antimicrobial wound dressings of multiple properties remains challenging for public health. Despite their various formats, the available developed dressings with limited functions may not fulfill the diverse demands involved in the complex wound healing process. In this study, multifunctional sandwich-structured electrospinning nanofiber membranes (ENMs) were fabricated. According to the structural composition, the obtained ENMs included a hydrophilic inner layer loaded with curcumin and gentamicin sulfate, an antibacterial middle layer consisting of bovine serum albumin stabilized silver oxide nanoparticles, and a hydrophobic outer layer. The prepared sandwich-structured ENMs (SNM) exhibited good biocompatibility and killing efficacy on Escherichia coli, Staphylococcus aureus, and Methicillin-resistant S. aureus (MRSA). In particular, transcriptomic analysis revealed that SNM inactivated MRSA by inhibiting its carbohydrate and energy metabolism and reduced the bacterial resistance by downregulating mecA. In the animal experiment, SNM showed improved wound healing efficiency by reducing the bacterial load and inflammation. Moreover, 16S rDNA sequencing results indicated that SNM treatment may accelerate wound healing without observed influence on the normal skin flora. Therefore, the constructed sandwich-structured ENMs exhibited promising potential as dressings to deal with the infected wound management.

Accelerated Wound Healing by Electrospun Multifunctional Fibers with Self-Powered Performance

“…As the largest multifunctional organ of the human body, skin serves as a natural barrier to protect against external stimuli and regulate body metabolism. − The treatment of skin damage is a common problem in daily life, and wound dressings are the primary clinical therapies to promote wound healing and maintain physical health. − While current dressings still have issues with inaccurate inflammation control and insufficient comprehensive functionality, which causes delayed healing. , It is important to analyze the healing mechanism and develop versatile dressings to enhance wound healing. Recent studies have shown that the endogenous electric field (EF), generated by the directional ion flow following skin injury, has a substantial impact on the stimulation of cell proliferation and migration .…”

Zinc Imidazole Framework‐8 Nanoparticles Disperse MRSA Biofilm by Inhibiting Arginine Biosynthesis and Down‐Regulating Adhesion‐Related Proteins

“…[17][18][19][20][21][22] The mechanism of antimicrobial nanomaterials enables them to eliminate resistant pathogenic microorganisms in environmental and healthcare applications, while down regulating their drug resistance genes and avoiding targeted resistance of bacteria, which is of great significance for controlling nosocomial infections and protecting public health. [23] Metal-organic framework (MOF) materials have been widely used for antimicrobial applications. [24][25][26][27] MOFs materials can also be used as capacitors, sensors, adsorption materials, catalytic materials, drug slow-release carriers, etc.…”