Encapsulated Pine Bark Polyphenolic Extract during Gastrointestinal Digestion: Bioaccessibility, Bioactivity and Oxidative Stress Prevention

Ferreira-Santos, Pedro; Ibarz, Raquel; Fernandes, Jean-Michel; Pinheiro, Ana Cristina; Botelho, C. M.; Rocha, Cristina M. R.; Teixeira, J. A.; Martı́n-Belloso, Olga

doi:10.3390/foods10020328

Cited by 22 publications

(19 citation statements)

References 44 publications

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“…Despite the abundance of flavonoids and other phenolic compounds found in S. nigra aqueous extract (Table 2), in our study S. nigra extracts seemed to be particularly effective against Gram-positive bacteria, namely against staphylococcal species, in opposite to Gram-negative. This distinct activity of phenolic extracts between Gram-positive and Gram-negative was also previously reported in the literature [2,92,93]. The mechanism of action of several compounds of plant extracts, like naringenin, quercetin and kaempferol might not be due to selectively bacteria targeting, due to membrane disruption [94].…”

Section: In-vitro Cell Assays and Antimicrobial Activitysupporting

confidence: 78%

“…The functionalization of foods and beverages with plant-based extracts, strongly affected the natural supplement market. This market registered an exponential growth in the production and commercialization of food supplements, especially those which are plant-based [2]. There is evidence that a diet rich in plant derivatives is usually associated with a lower risk of diseases, such as neurological disorders, diabetes, cancer, cardiovascular and inflammatory diseases [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Characterization of Sambucus nigra L. Flowers Aqueous Extract and Its Biological Implications

et al. 2021

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The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 °C). The extract obtained at 90 °C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was analyzed by GC-MS and HPLC-MS, which allowed the identification of 46 compounds, being quercetin and chlorogenic acid derivatives representative of 86% of the total of phenolic compounds identified in hydrophilic fraction of the aqueous extract. Naringenin (27.2%) was the major compound present in the lipophilic fraction. The antiproliferative effects of the S. nigra extract were evaluated using the colon cancer cell lines RKO, HCT-116, Caco-2 and the extract’s antigenotoxic potential was evaluated by the Comet assay in RKO cells. The RKO cells were the most susceptible to S. nigra flower extract (IC50 = 1250 µg mL−1). Moreover, the extract showed antimicrobial activity against Gram-positive bacteria, particularly Staphylococcus aureus and S. epidermidis. These results show that S. nigra-based extracts can be an important dietary source of bioactive phenolic compounds that contribute to health-span improving life quality, demonstrating their potential as nutraceutical, functional foods and/or cosmetic components for therapeutic purposes.

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Section: In-vitro Cell Assays and Antimicrobial Activitysupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Chemical Characterization of Sambucus nigra L. Flowers Aqueous Extract and Its Biological Implications

et al. 2021

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“…Individual phenolic compounds were analyzed by Shimadzu Nexera X2 UHPLC chromatograph equipped with Diode Array Detector (DAD) (Shimadzu, SPD-M20A, Kyoto, Japan) using a previously validated method, as described by Ferreira-Santos et al [25]. Separation was performed on a reversed phase Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 µm particle size; from Waters) and a precolumn of the same material at 40 • C. The flow rate was 0.4 mL/min.…”

Section: Identification and Quantification Of Individual Phenolic Commentioning

confidence: 99%

Chemical Profile and Bioactivities of Extracts from Edible Plants Readily Available in Portugal

Silva

Cadavez

Ferreira-Santos

et al. 2021

Foods

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Plant extracts have been proposed as alternative biocides and antioxidants to be included in a variety of food products. In this work, to assess the potential of rosemary, lemon balm, basil, tarragon, sage, and spearmint to be used as food additives, the chemical profiles and bioactivities of such plant extracts were studied. Furthermore, to evaluate the influence of extraction methods and solvents on the chemical characteristics and bioactivities of the plant extracts, two extraction methods (solid-liquid and Soxhlet extraction) and two solvents (water and ethanol 70% (v/v)) were tested for each plant. Groupwise summary statistics were calculated by plant, extraction method, and solvent, and linear models were built to assess the main effects of those terms and their interactions on the chemical characteristics and bioactivities of the extracts. The results revealed that all factors—type of plant, extraction method and solvent—have influence on the chemical profile and antioxidant activity of the resultant extracts. Interactions between factors were also observed. Hydroethanolic Soxhlet extracts presented the least potential as biopreservatives due to their low phenolic content and reduced antioxidant capacity. Oppositely, aqueous Soxhlet extracts and hydroethanolic solid-liquid extracts showed high contents in phenolic compounds and high antioxidant activities. In particular, the hydroethanolic solid-liquid extracts of lemon balm, spearmint, and sage presented the highest phenolic and flavonoid contents, accompanied by a high antioxidant activity, and they revealed antimicrobial activity against four pathogens (S. enterica ser. Typhimurium, E. coli, L. monocytogenes and S. aureus). These results demonstrate the potential of these natural resources to be incorporated as bioactive preservatives in foods or their packaging.

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“…Individual phenolic compounds were analyzed by Shimadzu Nexera X2 UHPLC chromatograph equipped with a Diode Array Detector (DAD) (Shimadzu, SPD-M20A, Kyoto, Japan) using a previously validated method, as described by Ferreira-Santos et al (2021a). Separation was performed on a reversed phase Acquity UPLC BEH C18 column (2.1 × 100 mm, 1.7 µm particle size; from Waters) and a precolumn of the same material at 40 • C. The flow rate was 0.4 mL/min.…”

Section: Identification and Quantification Of Individual Phenolic Compoundsmentioning

confidence: 99%

Extracts From Red Eggplant: Impact of Ohmic Heating and Different Extraction Solvents on the Chemical Profile and Bioactivity

Ferreira-Santos

Duca²,

Genisheva

et al. 2021

Front. Sustain. Food Syst.

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Eggplants contain a multitude of biocompounds with nutritional and/or biological activities. The objective of this work was to study the nutritional, chemical and bioactive value of red eggplant from Rotonda, Italy. Ohmic heating (OH) was compared to conventional heating, as different solvents were used (water, ethanol 30, 50, and 90% and methanol) for biocompounds extraction. Extracts were evaluated for their total phenolic compounds, antioxidant and antibacterial activities, and its toxicity was assessed in cells, L929 and Caco-2. The nutritional characterization of Rotonda's eggplant demonstrated that it is rich in carbohydrates (65%), fiber (12.5%), proteins (13%), lipids (7.6%) and minerals. Potassium is the mineral with the highest concentration in the red eggplant (27.24 mg/g). Phenolic composition of the obtained extracts was dependent on the extraction method, as well as on the solvent. The use of OH method increased the extraction of biocompounds, especially when using 50% of ethanol as solvent. The main phenolic compounds found in the extracts of this eggplant variety were ellagic acid, p-coumaricic acid, epicatechin, narginin, taxifolin and kaempferol. Antioxidant activity was positively correlated with the total amounts of phenolics. Red Eggplant extracts showed activity against Gram-negative bacteria (E. coli and S. enterica), however, they did not demonstrate activity against Gram-positive bacteria. The extracts obtained did not show cytotoxic effects in fibroblast and colorectal studied cells. Ohmic heating is a sustainable technology that increases the extraction yield of biocompounds, with reduced energy consumption and the resulting extracts show low toxicity and high biological activity.

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Encapsulated Pine Bark Polyphenolic Extract during Gastrointestinal Digestion: Bioaccessibility, Bioactivity and Oxidative Stress Prevention

Cited by 22 publications

References 44 publications

Chemical Characterization of Sambucus nigra L. Flowers Aqueous Extract and Its Biological Implications

Chemical Characterization of Sambucus nigra L. Flowers Aqueous Extract and Its Biological Implications

Chemical Profile and Bioactivities of Extracts from Edible Plants Readily Available in Portugal

Extracts From Red Eggplant: Impact of Ohmic Heating and Different Extraction Solvents on the Chemical Profile and Bioactivity

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