Encapsulated mesenchymal stem cells for in vivo immunomodulation

Zanotti, Lucia; Sarukhan, Adélaïda; Dander, Erica; Castor, Marina Gomes Miranda e; Cibella, Javier; Soldani, Cristiana; Trovato, Anna Elisa; Ploia, Cristina; Luca, Giovanni; Calvitti, Mario; Mancuso, Francesca; Arato, Iva; Golemac, Mijo; Jonjić, Nives; Biondi, Andrea; Calafiore, Riccardo; Locati, Massimo; D’Amico, Giovanna; Viola, Antonella

doi:10.1038/leu.2012.202

Cited by 70 publications

(58 citation statements)

References 18 publications

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“…Alginate has been used before to protect cells against the host immune system (Herrero et al, 2007;Kang et al, 2014;Zanotti et al, 2013). Although in our in vitro assay, alginate appeared to stimulate the proliferation of stimulated T cells, when MSCs were encapsulated, the alginate constructs inhibited T cell proliferation.…”

Section: Mjc Leijs Et Al Encapsulation Of Mscs In Alginatementioning

confidence: 52%

“…Alginate, a natural polysaccharide isolated from brown seaweed, is the most common used gel for cell encapsulation due to its biocompatibility and stability in vivo (de Vos et al, 2002;Shoichet and Rein, 1996;Wu et al, 2007;Murua et al, 2008). Moreover, alginate has the capacity to protect encapsulated cells against recognition by the immune system (Barminko et al, 2011;Herrero et al, 2007;Kang et al, 2014;Trouche et al, 2010;Zanotti et al, 2013), which will enable the use of allogeneic cells. This would greatly enhance the clinical translatability of MSCs-based therapies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Encapsulation of allogeneic mesenchymal stem cells in alginate extends local presence and therapeutic function

Leijs¹,

Villafuertes²,

Haeck³

et al. 2017

eCM

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Bone marrow derived mesenchymal stem cells (MSCs) have immunomodulatory and trophic capacities. For therapeutic application in local chronic inflammatory diseases, MSCs, preferably of allogeneic origin, have to retain immunomodulatory properties. This might be achieved by encapsulation of MSCs in a biomaterial that protects them from the host immune system. Most studies investigating the properties of MSCs for therapeutic application use short term cultures of cells in monolayer.Since the physical environment of MSCs can influence their functionality, we evaluated the feasibility of preserving the immunomodulatory properties of MSCs encapsulated in a three-dimensional alginate construct.After 5 weeks of implantation in immunocompetent rats, active allogeneic MSCs encapsulated in alginate were still detectable by Bio Luminescence Imaging and Magnetic Resonance Imaging of luciferase transduced and superparamagnetic iron oxide labelled MSCs. MSCs injected in saline were only detectable up to 1 week after injection. Moreover, the MSCs encapsulated in alginate responded to inflammatory stimuli similarly to MSCs in monolayer culture. In addition, MSC-alginate beads secreted immunomodulatory and trophic factors and inhibited T-cell proliferation after 30 d of in vitro culture. Our data indicate that allogeneic MSCs encapsulated in alginate persist locally and could act as an interactive immunomodulatory or trophic factor release system for several weeks, making this an interesting system to investigate for application in inflammatory disease conditions.

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Section: Mjc Leijs Et Al Encapsulation Of Mscs In Alginatementioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Encapsulation of allogeneic mesenchymal stem cells in alginate extends local presence and therapeutic function

Leijs¹,

Villafuertes²,

Haeck³

et al. 2017

eCM

View full text Add to dashboard Cite

show abstract

“…In animals that received alginate-encapsulated MSCs, a trend of decrease of the cumulative histologic score, inflammatory cell infiltration and tissue damage was revealed in the liver. The numerous foci of lymphoid cells infiltrating hepatic lobules characteristic of GVHD livers, were also reduced following treatment with encapsulated-MSCs [56]. Strikingly, GVHD mice that did not receive MSCs or received iv MSC injection died between day 6 and 13 after transplantation, while the treatment with subcutaneously-injected alginate-encapsulated MSCs significantly increased survival rate and clinical score of GVHD mice on day 8 [56].…”

Section: Immunomodulationmentioning

confidence: 82%

Bioengineering Alginate for Regenerative Medicine Applications

Ruvinov

Cohen

2016

Biomaterials From Nature for Advanced Devices and Therapies

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“…1), and successfully differentiated as directed (6). Alginate also exhibits excellent in vivo stability (29). However, multiple factors can influence alginate-based capsule stability after transplantation, such as the implantation site and capsule composition (30).…”

Section: Bioencapsulation Materials and Methodsmentioning

confidence: 99%

Bioencapsulation Technologies in Tissue Engineering

Majewski

Zhang

et al. 2016

Journal of Applied Biomaterials & Functional Materials

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Bioencapsulation technologies have played an important role in the developing successes of tissue engineering. Besides offering immunoisolation, they also show promise for cell/tissue banking and the directed differentiation of stem cells, by providing a unique microenvironment. This review describes bioencapsulation technologies and summarizes their recent progress in research into tissue engineering. The review concludes with a brief outlook regarding future research directions in this field.

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Encapsulated mesenchymal stem cells for in vivo immunomodulation

Abstract: Acute myeloid leukemia with biallelic CEBPA gene mutations and normal karyotype represents a distinct genetic entity associated with a favorable clinical outcome.

Cited by 70 publications

References 18 publications

Encapsulation of allogeneic mesenchymal stem cells in alginate extends local presence and therapeutic function

Encapsulation of allogeneic mesenchymal stem cells in alginate extends local presence and therapeutic function

Bioengineering Alginate for Regenerative Medicine Applications

Bioencapsulation Technologies in Tissue Engineering

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