Encapsulated enhanced green fluorescence protein in silica nanoparticle for cellular imaging

Cai, Zhengwei; Ye, Zhangmei; Yang, Xiaowei; Chang, Ya-Wen; Wang, Haifang; Liu, Yuanfang; Cao, Aoneng

doi:10.1039/c0nr00956c

Cited by 63 publications

(58 citation statements)

References 18 publications

(36 reference statements)

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“…Hence, direct encapsulating during particle formation usually leads to denaturation and concomitant loss of protein activity. As a consequence, only proteins with high stability, such as horseradish peroxidase, [ 8 ] green fl uorescent protein, [ 9,10 ] or myoglobin, [ 11 ] have so far been successfully encapsulated during particle formation. We will demonstrate below that these proteins are inappropriate models for testing biocompatible surface properties and mild immobilization methods due to their robust tertiary architecture.…”

Section: Doi: 101002/adma201503935mentioning

confidence: 99%

Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins

et al. 2015

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Section: Doi: 101002/adma201503935mentioning

confidence: 99%

Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins

et al. 2015

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“…Carboxyfluores ceinlabelled siRNA (FAMsiRNA) and primers were synthesized by Genepharma Co., Ltd (Shanghai, China). The expression and purification of the RFP were preformed following the previously reported procedure [23][24][25].…”

Section: Methodsmentioning

confidence: 99%

“…The stability of RFP@CS against protease digestion was evaluated by the proteinase K assay, following the similar procedure as the previous reports [23][24][25]. The flu orescence of RFP@CS in 6 M guanidine hydro chloride (GdHCl) was measured to examine the stability of encapsulated RFP against the protein denaturant.…”

Section: Synthesis Of Rfp@cs Np and Characterizationmentioning

confidence: 99%

Chitosan-Coated Red Fluorescent Protein Nanoparticle as a Potential Dual-Functional siRNA Carrier

Liu

Zhang

Deng

et al. 2015

Nanomedicine (Lond.)

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“…30 Since MSNs are much smaller than eukaryotic cells, they can facilitate the transport of proteins into the cytosol via an endocytosis pathway and subsequent endosomal escape. [31][32] Numerous synthetic protocols for the preparation of MSNs have been developed with the aim of controlling the size and morphology of these nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Mesoporous Silica Nanoparticles with Large Pores for the Encapsulation and Release of Proteins

Tu¹,

Boyle²,

Friedrich

et al. 2016

ACS Appl. Mater. Interfaces

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Mesoporous silica nanoparticles (MSNs) have been explored extensively as solid supports for proteins in biological and medical applications. Small (< 200 nm) MSNs with ordered large pores (> 5 nm), capable of encapsulating therapeutic small molecules suitable for delivery applications in vivo, are rare however. Here we present small, elongated, cuboidal, MSNs with average dimensions of 90 × 43 nm that possess disk-shaped cavities, stacked on top of each other, which run parallel to the short axis of the particle. Amine-functionalization was achieved by modifying the MSN surface with 3-aminopropyltriethoxysilane or 3-[2-(2-aminoethylamino)ethylamino] propyltrimethoxysilane (AP-MSNs and AEP-MSNs) and were shown to have similar dimensions to the non-functionalized MSNs. The dimensions of these particles, and their large surface areas as measured by nitrogen adsorption-desorption isotherms, make them ideal scaffolds for protein encapsulation and delivery. We therefore investigated the encapsulation and release behavior for seven model proteins (α-lactalbumin, ovalbumin, bovine serum albumin, catalase, hemoglobin, lysozyme and cytochrome c). It was discovered that all types of MSNs used in this study allow rapid encapsulation, with a high loading capacity, for all proteins studied. Furthermore, the release profiles of the proteins were tunable. The variation in both rate and amount of protein uptake and release was found to be determined by the surface chemistry of the MSNs, together with the isoelectric point (pI), and molecular weight of the proteins, as well as by the ionic strength of the buffer. These MSNs with their large surface area and optimal dimensions, provide a scaffold with a high encapsulation efficiency and controllable release profiles for a variety of proteins, enabling potential applications in fields such as drug delivery and protein therapy.

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Encapsulated enhanced green fluorescence protein in silica nanoparticle for cellular imaging

Cited by 63 publications

References 18 publications

Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins

Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins

Chitosan-Coated Red Fluorescent Protein Nanoparticle as a Potential Dual-Functional siRNA Carrier

Mesoporous Silica Nanoparticles with Large Pores for the Encapsulation and Release of Proteins

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