Enantioseparations in non-aqueous capillary electrochromatography using polysaccharide type chiral stationary phases

Girod, Marco; Chankvetadze, Bezhan; Blaschke, Gottfried

doi:10.1016/s0021-9673(99)01204-2

Cited by 76 publications

(93 citation statements)

References 19 publications

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“…Stationary phases previously employed in chiral HPLC, such as CDs and their derivatives [37,38], macrocyclic antibiotics [39], proteins [26], polysaccharides [40][41][42], and ligand-exchange type selectors [43] (see Table 1) have been adapted for chiral CEC using packed capillary columns. Open-tubular capillary columns have been the less used for the enantiomeric separation of chiral compounds by CEC.…”

Section: Cecmentioning

confidence: 99%

Sensitive chiral analysis by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

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Sensitive chiral analysis by capillary electrophoresisIn this review, an updated view of the different strategies used up to now to enhance the sensitivity of detection in chiral analysis by CE will be provided to the readers. With this aim, it will include a brief description of the fundamentals and most of the recent applications performed in sensitive chiral analysis by CE using offline and online sample treatment techniques (SPE, liquid-liquid extraction, microdialysis, etc.), on-column preconcentration techniques based on electrophoretic principles (ITP, stacking, and sweeping), and alternative detection systems (spectroscopic, spectrometric, and electrochemical) to the widely used UV-Vis absorption detection.

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Section: Cecmentioning

confidence: 99%

Sensitive chiral analysis by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

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“…Several groups recently investigated polysaccharide derivative-based CSPs for chiral CEC separations in aqueous [514][515][516][517] or non-aqueous medium [518]. Several drug enantiomers were resolved on these phases.…”

Section: Packed Capillariesmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

223

135

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This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

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“…The previous reports of CEC enantioseparations on this kind of coated CSPs were performed under the mobile phases of acetonitrile-water or pure methanol and ethanol systems [9][10][11][12][13][14][15][16][17]. However, the typical mobile phase used for enantioseparations on polysaccharide derivative CSPs is the hexane-alcohol system according to the knowledge in HPLC [8].…”

Section: Separation Of Enantiomers With Nonaqueous Phasesmentioning

confidence: 99%

“…Polysaccharide derivative-based chiral stationary phases (CSPs) have been shown the efficient chiral recognition ability for a variety of racemic compounds in HPLC and have been extensively studied [7,8]. Recently, polysaccharide derivatives were also applied as CSPs for CEC separation of enantiomers based on the open tubular and packed capillary columns [9][10][11][12]. For instance, capillary columns packed with cellulose tri (3,5-dimethylphenylcarbamate) and cellulose tri(3,5-dichlorophenylcarbamate) coated CSPs have been used for resolution of enantiomers by Chankvetadze et al [13][14][15][16][17], and the effect of the pore size of silica gel and the mobile phase composition on enantioseparations was systematically investigated.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer

et al. 2003

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Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer Positively charged chiral stationary phases (CSPs) were prepared for capillary electrochromatography (CEC) separation of enantiomers by chemically immobilizing cellulose derivatives onto diethylenetriaminopropylated silica (DEAPS) with tolylene-2,4-diisocyanate (TDI) as a spacer reagent. Anodic electroosmotic mobility was observed in both nonaqueous and aqueous mobile phases due to the positively charged amines on the surface of the prepared CSPs. For comparison, the traditionally used 3-aminopropyl silica (APS) was also adopted as the base material instead of DEAPS to prepare CSP. It was observed that the EOF on the DEAPS-based CSP was 18%-60% higher than that on the APS-based CSP under nonaqueous mobile phase conditions. Separation of enantiomers in CEC was performed on the positively charged CSPs with the nonaqueous mobile phases of pure ethanol or mixture of hexane-alcohol and the aqueous phases of acetonitrile-water or 95% ethanol. Fast separation of enantiomers was achieved on the newly prepared CSPs.

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Enantioseparations in non-aqueous capillary electrochromatography using polysaccharide type chiral stationary phases

Cited by 76 publications

References 19 publications

Sensitive chiral analysis by capillary electrophoresis

Sensitive chiral analysis by capillary electrophoresis

Chiral separation by chromatographic and electromigration techniques. A Review

Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer

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