Enantioseparation of N -derivatized amino acids by micro-liquid chromatography/laser induced fluorescence detection using quinidine-based monolithic columns

Wu, Huihui; Wang, Qiqin; Ruan, Meng; Peng, Kun; Zhu, Ping; Crommen, Jacques; Han, Hai; Jiang, Zhengjin

doi:10.1016/j.jpba.2015.12.011

Cited by 17 publications

(9 citation statements)

References 30 publications

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“…When the apparent pH was raised from 4.3 to 6.3, both the retention factor and resolution were enhanced [67]. In another study, when the apparent pH was increased from 5 to 6 increased retention, selectivity and resolution were found, similar to the results mentioned earlier [66]. These observations, in both cases, were explained by the protonation states of the SO and SAs: the QD-based CSP was thought to remain positively charged, while the negative charge of the derivatized amino acids increased with increasing pH.…”

Section: Effect Of Phsupporting

confidence: 83%

“…Carbamoylated QD-functionalized monolithic columns were prepared and tested in the enantioseparations of N-derivatized amino acids under micro-LC conditions using ammonium acetate or formate and ACN as eluent [63,66,67]. The higher the ACN content applied in the mobile phase the lower the retention was, which suggests a hydrophobic retention contribution for the monolithic CSPs.…”

Section: Effect Of Mobile Phase Compositionmentioning

confidence: 99%

“…Enantioselectivity and resolution were also significantly affected by the pH and both of them were improved at higher pHs. Monolithic columns functionalized with QD were employed for the enantiomeric resolution of N-derivatized amino acids [66,67]. When the apparent pH was raised from 4.3 to 6.3, both the retention factor and resolution were enhanced [67].…”

Section: Effect Of Phmentioning

confidence: 99%

See 2 more Smart Citations

Liquid chromatographic enantiomer separations applying chiral ion-exchangers based on Cinchona alkaloids

Ilisz

Bajtai

Lindner

et al. 2018

Journal of Pharmaceutical and Biomedical Analysis

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Section: Effect Of Phsupporting

confidence: 83%

Section: Effect Of Mobile Phase Compositionmentioning

confidence: 99%

Section: Effect Of Phmentioning

confidence: 99%

See 1 more Smart Citation

Liquid chromatographic enantiomer separations applying chiral ion-exchangers based on Cinchona alkaloids

Ilisz

Bajtai

Lindner

et al. 2018

Journal of Pharmaceutical and Biomedical Analysis

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“…R‐ (‐)‐1‐(1‐naphthyl)ethyl isocyanate was supplied by TCI Development Corporation (Shanghai, China). NBD‐derivatized amino acids were synthesized according to . All reagents were used without further purification.…”

Section: Methodsmentioning

confidence: 99%

Preparation and evaluation of tert‐leucine derivative functionalized polymeric monoliths for micro‐liquid chromatography

Ruan

Wang

et al. 2017

Electrophoresis

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In this study, a novel functional monomer N-[1-(α-naphthyl)ethylaminocarbonyl]-D-tert-leucine-[2-(methacryloyloxy)ethyl] amide (NA-D-tert-Leu-MA) was synthesized, and then employed to prepare polymeric monoliths (240 mm × 100 μm id) functionalized with tert-leucine derivative through a single step thermo-initiated co-polymerization approach or a multi-step post-modification approach. The multi-step approach involves the post-modification of a thiol-containing monolith with NA-D-tert-Leu-MA via "thiol-ene"click reaction. The physicochemical properties of the resulting monoliths were characterized by scanning electron microscopy, energy-dispersive X-ray spectrometry and micro-liquid chromatography. Good column stability, permeability, efficiency and reproducibility were obtained for the optimized monoliths. The poly (NA-D-tert-Leu-MA-co-ethylene dimethacrylate) monolith prepared through the single step co-polymerization approach exhibited satisfactory achiral separation performance for various analytes, including phenols, aniline derivatives and intact proteins, while its enantioseparation ability is rather poor. In contrast to that, the monolith prepared through the multi-step post-modification approach showed much higher enantioselectivity for 7-nitro-2,1,3-benzoxadiazole (NBD)-derivatized amino acids. Three NBD-derivatized amino acids (theanine, proline and norleucine) could be baseline enantioseparated.

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“…It showed fast analytical process for the enantioseparation of NBD‐derivatized amino acids compared with quinine or quinidine‐based packed columns. The effect of the co‐monomer hydroxyethyl methacrylate (HEMA) has been studied with no significant influence on the enantiorecognition ability of the quinidine‐based monolith was reported .…”

Section: Monolithic Stationary Phasesmentioning

confidence: 99%

An insight into chiral monolithic stationary phases for enantioselective high‐performance liquid chromatography applications

Fouad

Ibrahim

Adly

et al. 2019

J of Separation Science

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In this review, three main classes of chiral monolithic stationary phases, namely silica-, organic polymer-, and hybrid-based monolithic stationary phases, are covered. Their preparations, applications, and advantages compared with the conventionalpacked and open-tubular capillary columns are discussed. A detailed description of the different types and techniques used for the introduction of chiral selectors into the monolithic matrices such as immobilization, functionalization, coating, encapsulation, and bonding. Special emphasis is given to the recent developments of chiral selectors in HPLC monolithic stationary phases during the past 18 years.

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Enantioseparation of N -derivatized amino acids by micro-liquid chromatography/laser induced fluorescence detection using quinidine-based monolithic columns

Cited by 17 publications

References 30 publications

Liquid chromatographic enantiomer separations applying chiral ion-exchangers based on Cinchona alkaloids

Liquid chromatographic enantiomer separations applying chiral ion-exchangers based on Cinchona alkaloids

Preparation and evaluation of tert‐leucine derivative functionalized polymeric monoliths for micro‐liquid chromatography

An insight into chiral monolithic stationary phases for enantioselective high‐performance liquid chromatography applications

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