Enantioseparation and Determination of Propranolol in Human Plasma on a New Derivatized β-Cyclodextrin-Bonded Phase by HPLC

Ren-Dan, Zhou; Li, Laisheng; Cheng, Biaoping; Nie, Gui-Zhen; Zhang, Hongfu

doi:10.1016/s1872-2040(14)60751-9

Cited by 14 publications

(6 citation statements)

References 15 publications

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“…In this study, we have employed SERS combined with multivariate data analysis in order to develop a portable assay for the detection and quantification of propranolol at relevant concentration ranges found within multiple human biofluids (serum, plasma, and urine). The results achieved in this study clearly demonstrate the potential application of SERS for the detection of propranolol down to 133.1 ng/mL, which is comparable to commonly used techniques such as HPLC, while having the advantage that it is highly portable and could be developed for on-site testing.…”

supporting

confidence: 54%

“…In plasma the most intensive peak at 634 cm –1 can be assigned to l -tyrosine (C–S), and as for urine the main contribution was from uric acid at 658 and 1133 cm –1 . Although the LOD of propranolol using SERS is not as low as other commonly used techniques such as GC (0.05 ng/mL), HPLC (1 μg/L), and CE (0.01 μg/mL), SERS provides the advantage of being a highly portable, rapid, and cost-effective method, which can be used for the detection of various drugs in biofluid samples. In conclusion, our results clearly demonstrate for the first time that the detection and quantification of propranolol in multiple human biofluids using SERS is a promising analytical approach.…”

Section: Discussionmentioning

confidence: 99%

“…There is a vast amount of patient-specific information present in biofluids, and thus they provide vital medical information that can be utilized for diagnostic purposes, such as by providing patient-specific baselines of biomolecules, determination of pharmaceutical dosage, pharmacodynamics, and pharmacokinetics, as well as monitoring drug compliance and response. Propranolol, which is a β-adrenergic blocker (more commonly termed a beta-blocker), is used for the treatment and/or prevention of various disorders including hypertension, anxiety, convulsions, arrhythmias, migraine, and angina pectoris. , The detection of propranolol in human body fluids such as serum, plasma, and urine is commonly achieved via the use of gas chromatography (GC), high performance liquid chromatography (HPLC), and capillary electrophoresis (CE) . However, these techniques are time-consuming, can require long pretreatment steps to enrich the sample for the target analyte, incur the use of expensive reagents, and involve high instrumental costs.…”

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confidence: 99%

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Rapid, Accurate, and Quantitative Detection of Propranolol in Multiple Human Biofluids via Surface-Enhanced Raman Scattering

et al. 2016

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There has been an increasing demand for rapid and sensitive techniques for the identification and quantification of pharmaceutical compounds in human biofluids during the past few decades, and surface-enhanced Raman scattering (SERS) is one of a number of physicochemical techniques with the potential to meet these demands. In this study we have developed a SERS-based analytical approach for the assessment of human biofluids in combination with chemometrics. This novel approach has enabled the detection and quantification of the β-blocker propranolol spiked into human serum, plasma, and urine at physiologically relevant concentrations. A range of multivariate statistical analysis techniques, including principal component analysis (PCA), principal component-discriminant function analysis (PC-DFA) and partial least-squares regression (PLSR) were employed to investigate the relationship between the full SERS spectral data and the level of propranolol. The SERS spectra when combined with PCA and PC-DFA demonstrated clear differentiation of neat biofluids and biofluids spiked with varying concentrations of propranolol ranging from 0 to 120 μM, and clear trends in ordination scores space could be correlated with the level of propranolol. Since PCA and PC-DFA are categorical classifiers, PLSR modeling was subsequently used to provide accurate propranolol quantification within all biofluids with high prediction accuracy (expressed as root-mean-square error of predictions) of 0.58, 9.68, and 1.69 for serum, plasma, and urine respectively, and these models also had excellent linearity for the training and test sets between 0 and 120 μM. The limit of detection as calculated from the area under the naphthalene ring vibration from propranolol was 133.1 ng/mL (0.45 μM), 156.8 ng/mL (0.53 μM), and 168.6 ng/mL (0.57 μM) for serum, plasma, and urine, respectively. This result shows a consistent signal irrespective of biofluid, and all are well within the expected physiological level of this drug during therapy. The results of this study demonstrate the potential of SERS application as a diagnostic screening method, following further validation and optimization to improve detection of pharmaceutical compounds and quantification in human biofluids, which may open up new exciting opportunities for future use in various biomedical and forensic applications.

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confidence: 54%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Rapid, Accurate, and Quantitative Detection of Propranolol in Multiple Human Biofluids via Surface-Enhanced Raman Scattering

et al. 2016

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“…Based on these observations, the dimensional coefficient can be used as a selector that determines the type of plot of ΔE ads versus the type of CD. We took the ΔE ads values obtained as the theoretical enantioselectivity and compared it with the experimental enantioselectivity values (Tables 5-7) available in the previously published electrochemical 28,29,[33][34][35][36][37][38][39][40][41][42][43][44] or chromatographic [63][64][65][66][67][68] studies. First, we considered the correlation with a strict selection of pairs of values, that is, maximum match of the type of chiral modifier in experiments and calculations (Figure 7, Table 5).…”

Section: Plot (Ii)mentioning

confidence: 99%

Rational design of highly enantioselective composite voltammetric sensors using a computationally predicted chiral modifier

et al. 2022

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The use of chiral modifiers is among the simplest and most popular strategies for synthesizing enantioselective voltammetric sensors that are applied for the analysis and discrimination of enantiomerical drugs in various media. The type and structure of the chiral modifier are the key factors for the creation of enantioselectivity to a specified analyte. We suggest a novel approach to the prediction of the quality of a chiral modifier for preparing highly enantioselective sensors. The suggested approach is based on the molecular mechanics modeling of the adsorption of analyte enantiomers on chiral modifiers and on the comparison of the corresponding adsorption energies (ΔEads). The efficiency of our approach is demonstrated using the example of cyclodextrins and chiral single‐wall carbon nanotubes as chiral modifiers, and a wide range of chiral analytes. We found that the experimental enantioselectivity (ϑexp) measured using voltammetry linearly correlates with ΔEads. The suggested approach also showed good predictive power in application to enantioselective chromatography, which further validates its general applicability.

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“…Besides those commercial CSPs, during the last few decades, significant progresses have been made on the development of home-made CSPs used for the direct separation of β-blockers in HPLC, mainly polysaccharide derivatives [ 36 , 37 , 38 , 39 , 40 , 41 ], cyclodextrins [ 42 , 43 , 44 , 45 , 46 , 47 ], macrocyclic antibiotic [ 35 ], chiral crown ethers [ 48 , 49 ], and Pirkle type CSPs [ 50 , 51 ].…”

Section: Enantioseparation Of β-Blockers By Hplcmentioning

confidence: 99%

Progress in the Enantioseparation of β-Blockers by Chromatographic Methods

et al. 2021

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β-adrenergic antagonists (β-blockers) with at least one chiral center are an exceedingly important class of drugs used mostly to treat cardiovascular diseases. At least 70 β-blockers have been investigated in history. However, only a few β-blockers, e.g., timolol, are clinically marketed as an optically pure enantiomer. Therefore, the separation of racemates of β-blockers is essential both in the laboratory and industry. Many approaches have been explored to obtain the single enantiomeric β-blocker, including high performance liquid chromatography, supercritical fluid chromatography and simulated moving bed chromatography. In this article, a review is presented on different chromatographic methods applied for the enantioseparation of β-blockers, covering high performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC) and simulated moving bed chromatography (SMB).

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Enantioseparation and Determination of Propranolol in Human Plasma on a New Derivatized β-Cyclodextrin-Bonded Phase by HPLC

Cited by 14 publications

References 15 publications

Rapid, Accurate, and Quantitative Detection of Propranolol in Multiple Human Biofluids via Surface-Enhanced Raman Scattering

Rapid, Accurate, and Quantitative Detection of Propranolol in Multiple Human Biofluids via Surface-Enhanced Raman Scattering

Rational design of highly enantioselective composite voltammetric sensors using a computationally predicted chiral modifier

Progress in the Enantioseparation of β-Blockers by Chromatographic Methods

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