Enantioselectivity of bovine serum albumin-bonded columns produced with isolated protein fragments

Haginaka, Jun; Kanasugi, Naoko

doi:10.1016/s0021-9673(97)00025-3

Cited by 26 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another application of zonal elution has involved the development of quantitative structure-retention relationships (QSRRs) that characterize the binding of drugs and other solutes to protein-based CSPs [41–43]. In this type of study, a set of retention parameters are acquired for a series of analytes and compared to various structural descriptors for these solutes to see which of these factors influence retention [42].…”

Section: Chromatographic Methods Used To Study Protein-based Chiramentioning

confidence: 99%

Chromatographic Studies of Protein-Based Chiral Separations

Zheng

Azaria

et al. 2016

Separations

View full text Add to dashboard Cite

The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha1-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations.

show abstract

Section: Chromatographic Methods Used To Study Protein-based Chiramentioning

confidence: 99%

Chromatographic Studies of Protein-Based Chiral Separations

Zheng

Azaria

et al. 2016

Separations

View full text Add to dashboard Cite

show abstract

“…Nakamura et al [198,200] prepared a BSA-multilayer-adsorbed porous hollow fiber membrane as a CSP. Haginaka and Kanasugi [201] isolated a BSA fragment of molecular mass 35236 and resolved 2-arylpropionic acid derivatives, benzodiazepines, warfarin and benzoin on a CSP prepared from this fragment.…”

Section: Chiral Imprinted Polymersmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

225

135

View full text Add to dashboard Cite

This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

show abstract

“…Macrocyclic antibiotic-based chiral columns hyphenated with mass spectrometry have also been tested for enantioselective separation of oxazepam [21]. Avidin [8], ovomucoid [9], bovine serum albumin [10], or human serum albumin [11] based CSPs have been reported to give satisfactory enantioresolution of lorazepam and oxazepam. Benzodiazepine derivatives have been also employed in a chromatographic study of binding sites of human serum albumin [11].…”

Section: Introductionmentioning

confidence: 99%

Comparison of enantioseparation of selected benzodiazepine and phenothiazine derivatives on chiral stationary phases based on β‐cyclodextrin and macrocyclic antibiotics

Tesařová¹,

Bosáková

2003

J of Separation Science

View full text Add to dashboard Cite

Comparison of enantioseparation of selected benzodiazepine and phenothiazine derivatives on chiral stationary phases based on b-cyclodextrin and macrocyclic antibiotics Enantioselective separation of some phenothiazine and benzodiazepine derivatives was studied on six different chiral stationary phases (CSPs) in HPLC. Selected CSPs, with respect to the structure of the separated compounds, were either based on b-cyclodextrin chiral selectors -underivatized b-cyclodextrin and hydroxypropyl ether b-cyclodextrin, or on macrocyclic antibiotics -vancomycin, teicoplanin, teicoplanin aglycone, and ristocetin A. Measurements were carried out in a reversedphase separation mode. The influence of mobile phase composition on retention and enantioseparation was studied. Benzodiazepines could be enantioresolved with almost all the chiral stationary phases used, except for the vancomycin-bonded CSP. Peak coalescence of oxazepam and lorazepam was observed if separation was carried out at laboratory temperature. Reduced temperature was required in some instances in order to avoid the on-column racemization. Separation systems composed of teicoplanin-bonded CSP and buffer-methanolic or pure methanolic mobile phases were shown to be suitable even for preparative purposes due to high resolution values of the enantiomers. Enantioseparation of phenothiazine derivatives was more difficult to achieve but it was successful, at least partly, also with both types of the CSPs used (except for levomepromazine).

show abstract

Enantioselectivity of bovine serum albumin-bonded columns produced with isolated protein fragments

Cited by 26 publications

References 17 publications

Chromatographic Studies of Protein-Based Chiral Separations

Chromatographic Studies of Protein-Based Chiral Separations

Chiral separation by chromatographic and electromigration techniques. A Review

Comparison of enantioseparation of selected benzodiazepine and phenothiazine derivatives on chiral stationary phases based on β‐cyclodextrin and macrocyclic antibiotics

Contact Info

Product

Resources

About