Enantioselective Separation of Antiretroviral Drug Combinations on Immobilized Polysaccharide CSPs Under Subcritical Conditions Using Supercritical Fluid Chromatography Apparatus

An overview of chiral separations of pharmaceutically active substances by HPLC (2018–2020)

“…Four different cellulose- and amylose-based columns were tested to investigate the retention behavior of several antiretroviral drugs. The retention mechanism was shown to depend on the chemical structure of the compounds and no universal column could be found for successful enantioseparation of all analytes [ 41 ]. It was found that lamivudine, efavirenz, and darunavir were retained more on cellulose-based stationary phases due to the increased involvement of hydrogen bonds.…”

“…It was found that lamivudine, efavirenz, and darunavir were retained more on cellulose-based stationary phases due to the increased involvement of hydrogen bonds. On the other hand, abacavir, dolutegravir, tenofovir, and emtricitabine were better resolved on amylose-based stationary phases in which the dominant retention mechanism is associated with the presence of well-defined cavities involved in steric interactions with analytes [ 41 ]. In addition to the separation of econazole by electrokinetic chromatography with hydroxypropyl-β-CD and tetrabutylammonium l -lysine [ 21 ], another new study focused on the separation of econazole enantiomers extracted from rat plasma by HPLC with tandem mass spectrometric detection (HPLC–MS/MS) [ 42 ].…”