Enantioselective separation and determination of the dinotefuran enantiomers in rice, tomato and apple by HPLC

Abstract

An effective chiral analytical method was developed for the resolution and determination of dinotefuran enantiomers in rice, tomato and apple samples. Dinotefuran enantiomers were baseline-separated and determined on a novel chiral column, ChromegaChiral CCA, with n-hexane-ethanol-methanol (85:5:10, v/v/v) as the mobile phase at a flow rate of 1.0 mL/min with UV detection at 270 nm. The resolution of dinotefuran enantiomers was about 1.8. The first eluted enantiomer was (+)-dinotefuran and the second eluted one was (-)-dinotefuran. The effects of mobile-phase composition and column temperature on the enantioseparation were evaluated. The method was validated for linearity, repeatability, accuracy, LOD and LOQ. LOD was 0.15 mg/kg in rice and tomato, 0.05 mg/kg in apple, with an LOQ of 0.5 mg/kg in rice and tomato, 0.2 mg/kg in apple. The average recoveries of the pesticide from all matrices ranged from 75.8 to 92.9% for all fortification levels The precision values associated with the analytical method, expressed as RSD values, were <16.5% for the pesticide in all matrices. The methodology was successfully applied for the enantioselective analysis of dinotefuran enantiomers in real samples, indicating its efficiency in investigating the environmental stereochemistry of dinotefuran in food matrix.

Enantioselective Olfactory Effects of the Neonicotinoid Dinotefuran on Honey Bees (Apis mellifera L.)

“…The chiral neonicotinoid insecticide dinotefuran has an asymmetrically substituted carbon (C) atom and consists of a pair of enantiomers, and it is used to control the growth of Delphacidae , Aphidoidea , Cicadellidae , stink bugs, and other pests on rice, vegetables, and fruit. − The stereoisomers of chiral dinotefuran may exhibit different behaviors in terms of their environmental, physical, and chemical properties, such as bioactivity, toxicity, and metabolism within organisms. − Some data have suggested that R -dinotefuran could minimize the hazard to honeybees when dinotefuran is used to control target pests . Consequently, a study on the toxicity of chiral dinotefuran enantiomers to honey bees is required.…”

Rapid and Sensitive Determination of Dinotefuran Residue Based on Electrochemical Enhancement of β‐cyclodextrin‐Graphene Composite

“…Taking into account the dilution of the sample, it was calculated that the LOD of DNF in the samples was 0.01 mg kg −1 . Compared with HPLC determination of DNF enantiomers in rice samples (LOD=0.15 mg kg −1 ) followed by solid phase dispersion extraction (SPE) , the new method fails to seperate the enantiomers. Nevertheless, the sensitivity of the proposed sensor is extremely low, attributed to the high electrochemical signal of DNF on the modified GCE, as well as the simple and effective pretreatment.…”

Rational design of a molecularly imprinted polymer for dinotefuran: theoretical and experimental studies aimed at the development of an efficient adsorbent for microextraction by packed sorbent

“…S5(A) † presents the initial tests for the chromatographic separation of the DNF enantiomers employing hexane : ethanol : methanol (85 : 5 : 10, v/v/v) at a flow rate of 1.0 mL min −1 as described in the literature. 38 Although the chiral selectors of the ChromegaChiral CCA (a polysaccharidecoated chiral stationary phase) and Chiralpak® IA (a polysaccharide-immobilized chiral stationary phase) columns were the same, both formed by 3,5-dimethylphenyl carbamate amylose, the chromatographic behaviours were different. A baseline separation was not observed.…”