Enantioselective reversed-phase and non-aqueous capillary electrochromatography using a teicoplanin chiral stationary phase

Karlsson, Charlotte; Wikström, Håkan; Armstrong, Daniel W.; Owens, Paul K.

doi:10.1016/s0021-9673(00)00805-0

Cited by 74 publications

(43 citation statements)

References 38 publications

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“…for 3.5 and 5 mm microparticles, respectively. The measured EOF was comparable to that measured by Karlsson et al [10] using a TE-CSP. Table 1 shows the data referring to type A capillaries, measured analyzing the 13 racemic mixtures of the studied basic compounds.…”

Section: Resultssupporting

confidence: 86%

“…1a), clearly showing that under the operating conditions the thermal and double layer overlap effects are negligible [10,29]. From Fig.…”

Section: Resultsmentioning

confidence: 69%

“…The enantiomeric resolution is achieved in a chiral environment where the selector is interacting with analytes by forming labile diastereomeric complexes (direct resolution method) under the influence of a relatively high electric field. The chiral selector can be added to the mobile phase [8] or bonded to (i) the stationary phase (packed columns) [9][10][11][12], (ii) a polymer (monolithic) [13] or the capillary wall (o-CEC) [14].…”

Section: Introductionmentioning

confidence: 99%

“…[16]. The same chiral selectors (TE and vancomycin) were also tested in CEC for the separation of mainly basic compounds [9,10,13,[17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of teicoplanin chiral stationary phases of 3.5 and 5 μm inside diameter silica microparticles by polar‐organic mode capillary electrochromatography

et al. 2003

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Different types of fused-silica capillaries of 75 microm inside diameter (ID) were packed, namely type A and B, and evaluated for the direct resolution of racemates of several basic compounds by enantioselective capillary electrochromatography (e-CEC). Type A was packed with a chiral stationary phase (CSP) containing teicoplanin (TE) mixed with silica microparticles (3:1 w/w) while type B contained only the TE-CSP. In both cases, particles of different sizes (3.5 and 5 microm ID) were employed. A polar-organic mobile phase containing methanol-acetonitrile (60-40% v/v and 0.05% w/v ammonium acetate was used. Several beta-blockers (alprenolol, oxprenolol, metoprolol, pindolol, salbutamol, propranolol, atenolol, acebutolol) were baseline-enantioresolved with both capillary types, in very short times.

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Section: Resultssupporting

confidence: 86%

“…1a), clearly showing that under the operating conditions the thermal and double layer overlap effects are negligible [10,29]. From Fig.…”

Section: Resultsmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

“…[16]. The same chiral selectors (TE and vancomycin) were also tested in CEC for the separation of mainly basic compounds [9,10,13,[17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of teicoplanin chiral stationary phases of 3.5 and 5 μm inside diameter silica microparticles by polar‐organic mode capillary electrochromatography

et al. 2003

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“…These phases were applied to the chiral separation of neutral, basic and acidic compounds in both reversed phase and polar organic mode with remarkable efficiency. Carter-Finch and Smith [522] and Karlsson et al [523] investigated capillaries packed with a commercial teicoplanin CSP (Chirobiotic T). It was shown that this phase can be used for the chiral separation of a broad spectrum of compounds using reversed phase and nonaqueous conditions.…”

Section: Packed Capillariesmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

225

135

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This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

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Chiral Separations

Schmid

2007

Kirk-Othmer Encyclopedia of Chemical Technology

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The development of chiral separation methods became very important in recent years since it is known that the biological or pharmacological activities of compounds of biological or pharmaceutical interest is mostly restricted to one of the enantiomers. Starting in the late 1960s with classical column liquid chromatography and the first direct gc separation, subsequently methods for HPLC and TLC were developed. More recently, electromigration techniques such as capillary zone electrophoresis, capillary electrokinetic chromatography and capillary electrochromatography found application for chiral separation. A broad spectrum of chiral separation principles have been developed in the 1970s, 1980s and first half of the 1990s, in which mainly applications of these basic separation principles were published. Several thousand publications appeared in this field. Miniaturized systems such as micro‐chip capillary electrophoresis attracted increasing attention in recent years. On the other hand, preparative HPLC found access into industrial production of enantiopure drugs. This article gives an overview of the diversity of chiral separation principles and their mechanisms. Due to the nature of stereoselectivity, there will never exist a universal chiral separation method, the choice of the method always has to be adapted to the problem. However, a few predictions are possible. This article does list applications, beacuse of space constrainds.

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Enantioselective reversed-phase and non-aqueous capillary electrochromatography using a teicoplanin chiral stationary phase

Cited by 74 publications

References 38 publications

Evaluation of teicoplanin chiral stationary phases of 3.5 and 5 μm inside diameter silica microparticles by polar‐organic mode capillary electrochromatography

Evaluation of teicoplanin chiral stationary phases of 3.5 and 5 μm inside diameter silica microparticles by polar‐organic mode capillary electrochromatography

Chiral separation by chromatographic and electromigration techniques. A Review

Chiral Separations

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