Abstract:Abstract. Xylene oxygenase (XO), an enzyme system from Pseudomonas putida mt-2, selectively oxidizes the methyl side group of toluene-derived compounds. The enzyme is related to Pseudomonas oleouorans mono-oxygenase (POM), which has been used to produce optically active epoxides from various alkenes at high enantiomeric excess. We have introduced XO into Escherichia coli and produced variously substituted benzyl alcohols from toluene derivatives and optically active styrene epoxides from styrene, m-and p-chlor… Show more
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