Enantioselective high performance liquid chromatography and supercritical fluid chromatography separation of spirocyclic terpenoid flavor compounds

Schaffrath, Mathias; Weidmann, Verena; Maison, Wolfgang

doi:10.1016/j.chroma.2014.07.001

Cited by 19 publications

(9 citation statements)

References 55 publications

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“…Volatile terpenoids are still nowadays separated by GC. Recent alternative developments using HPLC and SFC conditions haveproven efficient in separating enantiomeric forms of spirocyclicterpenoids, such as the aspirane and vitispirane, responsible for flavors used in industry [ 58 ]. Triterpenoids, such as betulinic, oleanolic, polpunonic and ursolic acids have been separated by SFC-FID [ 59 ].…”

Section: Applications To Lipid Analysismentioning

confidence: 99%

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Lipidomics by Supercritical Fluid Chromatography

Laboureur

Ollero

Touboul

2015

IJMS

100

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This review enlightens the role of supercritical fluid chromatography (SFC) in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography (LC). It is only 20 years later that a regain of interest appeared when new commercial instruments were introduced. As SFC is fully compatible with the injection of extracts in pure organic solvent, this technique is perfectly suitable for lipid analysis and can be coupled with either highly universal (UV or evaporative light scattering) or highly specific (mass spectrometry) detection methods. A short history of the use of supercritical fluids as mobile phase for the separation oflipids will be introduced first. Then, the advantages and drawbacks of SFC are discussed for each class of lipids (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, prenols, polyketides) defined by the LIPID MAPS consortium.

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Section: Applications To Lipid Analysismentioning

confidence: 99%

“…Within the field of lipids, the separation of chiral spirocyclicterpenoid molecules, of high interest in nutritional industry, has been successful following SFC-based protocols [ 58 ], due to the high volatility and low polarity of these compounds. Likewise, carotenoid structural isomers can be resolved by ODS column-based SFC-MS.…”

Section: Perspectivesmentioning

confidence: 99%

Lipidomics by Supercritical Fluid Chromatography

Laboureur

Ollero

Touboul

2015

IJMS

100

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“…Unfortunately, the only work mentioning SFC/ESCi‐MS provides no details regarding APCI‐mode . Available reports on packed column SFC analysis of volatile flavor compounds were restricted to PDA detection .…”

Section: Introductionmentioning

confidence: 99%

Investigation of chromatographic peak broadening in supercritical fluid chromatography/atmospheric pressure chemical ionization mass spectrometry

Pauk

Lemr

2020

J of Separation Science

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Multimode ionization source allows for switching between different ionization techniques, for example, electrospray and atmospheric pressure chemical ionization, within a single analysis. Supercritical fluid chromatography can handle a wide polarity range of substances from hydrophilic to lipophilic in a single run and can undoubtedly benefit from versatility of this ion source. Nevertheless, we observed a significant chromatographic peak broadening effect in atmospheric pressure chemical ionization mode during supercritical fluid chromatography‐mass spectrometry analysis of volatile flavor compounds with a dual ion source named ESCi (Waters). Surprisingly, this effect was not related to the separation process but was triggered solely by the ion source conditions. Neither of photodiode array detector, electrospray mode nor a dedicated atmospheric pressure chemical ionization source suffered from such a phenomenon. Chromatographic peak profiles of ten test substances obtained with the dual ion source were compared with photodiode array detector data as a reference. The broadening effect was more pronounced for volatile compounds with low polarity. Dependence of peak broadening on the ion source settings was systematically investigated. Tuning of desolvation gas flow and its temperature dramatically reduced peak distortion and increased detection sensitivity.

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“…2) SFC can be achieved quickly and with high-resolution separation when compared with HPLC separations because the diffusion of solutes in the supercritical fluids is about 10 times greater than that in liquids. 2) Thus, SFC is now commonly and widely used to perform chiral and/or achiral separations and purifications for the analysis of agrichemicals, [3][4][5] drugs, 6,7) foods, [8][9][10] flavors, 11) fossil fuels, 12) and natural products. 13) In the analysis of the above-mentioned fields, the development of a suitable SFC method with satisfactory precision is significant to the obtaining of reliable results.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Repeatability in Supercritical Fluid Chromatography with Electrochemical Detection Based on the ISO 11843 Part 7

Yamamoto

Kotani

Hayashi³

et al. 2019

CHEMICAL & PHARMACEUTICAL BULLETIN

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The present study proposes a method for the assessment of repeatability in supercritical fluid chromatography with electrochemical detection (SFC-ECD), based on the ISO 11843 part 7 (ISO 11843-7:2018) which can theoretically provide detection limits and standard deviation (S.D.) through the stochastic properties of baseline noise without repetitive measurements of real samples. On the baseline noise of SFC-ECD, large-amplitude and periodic noises with less than 0.05 Hz were observed, and the power spectrum of the baseline noise showed 1/f fluctuation ( f frequency). It was found that the present power spectrum analysis, according to the law of error propagation, can provide suitable noise parameters to calculate S.D. of baseline noise and a relative S.D. (RSD) of peak area by ISO 11843-7. The chromatographic determinations of α-, β-, γ-and δ-tocopherol have been taken as examples. In the present SFC-ECD, the RSDs of peak areas for α-, β-, γ-and δ-tocopherol obtained by ISO 11843-7 were within 95% confidence intervals of the RSD of them obtained by repetitive measurements (n 6). Thus, we found that ISO 11843-7 is applicable to the assessment of repeatability in SFC-ECD for determining tocopherols without repetitive measurements.

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Enantioselective high performance liquid chromatography and supercritical fluid chromatography separation of spirocyclic terpenoid flavor compounds

Cited by 19 publications

References 55 publications

Lipidomics by Supercritical Fluid Chromatography

Lipidomics by Supercritical Fluid Chromatography

Investigation of chromatographic peak broadening in supercritical fluid chromatography/atmospheric pressure chemical ionization mass spectrometry

Assessment of Repeatability in Supercritical Fluid Chromatography with Electrochemical Detection Based on the ISO 11843 Part 7

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