Enantioselective determination of felodipine and other chiral dihydropyridine calcium entry blockers in human plasma

Enantioselective determination of isradipine in human serum using chiral stationary phase liquid chromatography and gas chromatography with nitrogen selective detection

“…It was, however, not possible to remove interfering peaks in human serum extracts (Christensen et al, unpublished data), either by changing the analytical column and/ or the mobile phase, or by optimizing the extraction procedure using different solid phase columns. We then tried to separate the enantiomers with GC equipment as 8 Since no single chromatographic system could be optimized for measuring isradipine, we developed a method based on a combination of chiral column HPLC 9 and GC with nitrogen-selective detection.…”

Felodipine quantification in human plasma by high-performance liquid chromatography coupled to tandem mass spectrometry

“…Several analytical methods based on high resolution gas chromatography (HRGC) mainly with electron capture detector [4][5][6], high performance liquid chromatography (HPLC) [7][8][9][10], HRGC coupled to mass spectrometry (HRGC-MS) [11][12][13] and HPLC coupled to mass spectrometry (HPLC-MS) [14] and recently by HPLC coupled to tandem mass spectrometry (HPLC-MS-MS) [15] has been used for the felodipine quantitation in plasma. These methods however, not are ideal to pharmacokinetics studies, because are laborious and include time-consuming procedures or long chromatographic run times (>10 min) [14].…”

Redox properties of isradipine and its electrochemical detection in the HPLC determination of the compound in human serum

“…Reported methods for the chromatographic determination of isradipine include HPLC-UV (Boutagy et al, 1989), gas chromatography with chemical ionization mass spectrometry (Jean and Lablanche, 1988) and enantioselective determinations (Okamoto et al, 1990;Soons et al, 1990). Previous HPLC determinations of drugs of this type have mainly been conducted using UV detection (Wu et al, 1984;Kobayashi, 1987;Nitsche et al, 1987;Griener et al, 1988;Boutagy et al, 1989;Chauhwei and William, 1989;Bocker et al, 1990;Logan and Patrick, 1990;Nonzioli et al, 1990;Bealieu et al, 1991Bealieu et al, , 1992Gabrielsson et al, 1992;Qian and Gallo, 1992).…”