Enantiomeric resolution of some 2-arylpropionic acids using l-(−)-serine-impregnated silica as stationary phase by thin layer chromatography

Aboul‐Enein, Hassan Y.; El-Awady, Mahmoud I.; Heard, Charles Martin

doi:10.1016/s0731-7085(03)00208-5

Cited by 30 publications

(14 citation statements)

References 11 publications

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“…For enantiomeric separation, chiral stationary phases and mobile phase additives can be used. Brucine, chondroitin, heparin and HP-β-CD were used as chiral selectors for enantiomeric separation of different pharmaceutical compounds using TLC, LC and CE 26–28…”

Section: Resultsmentioning

confidence: 99%

“…Due to it’s lower health risks, HP-β-CD was chosen over brucine sulphate for the determination of escitalopram. We also investigated the effect of pH and temperature on resolution of racemic citalopram as they have been known to affect chiral recognition 26. The best conditions for discrimination of citalopram enantimers were found at pH 8.0 and 25 ± 2 °C.…”

Section: Resultsmentioning

confidence: 99%

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Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (±) Citalopram and its S - Enantiomer Escitalopram

2009

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Two sensitive and validated methods were developed for determination of a racemic mixture citalopram and its enantiomer S-(+) escitalopram. The first method was based on direct measurement of the intrinsic fluorescence of escitalopram using sodium dodecyl sulfate as micelle enhancer. This was further applied to determine escitalopram in spiked human plasma, as well as in the presence of common and co-administerated drugs. The second method was TLC densitometric based on various chiral selectors was investigated. The optimum TLC conditions were found to be sensitive and selective for identification and quantitative determination of enantiomeric purity of escitalopram in drug substance and drug products. The method can be useful to investigate adulteration of pure isomer with the cheap racemic form.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (±) Citalopram and its S - Enantiomer Escitalopram

2009

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“…It has many applications in the field of pharmaceutical studies which include: stability, impurities, synthetic drugs, pharmacokinetic, enantiomeric purity and drug monitoring in biological fluids (Mincsovics et al, 2001) (Aboul Enein et al, 2003. Accordingly HPTLC became a method of choice to use for analysis of our presented mixtures.…”

Section: Discussionmentioning

confidence: 99%

Stability indicating HPTLC method for determination of Metopimazine in pharmaceutical formulation and human plasma

Naguib

Abdelrahman

2014

Beni-Suef University Journal of Basic and Applied Sciences

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Oxidative degradationAlkaline degradation HPTLC Pharmaceutical formulation a b s t r a c t Accurate, selective and sensitive stability indicating high performance thin layer chromatographic (HPTLC) method was developed and validated for determination of Metopimazine (MPZ) in presence of its degradation products; including the oxidative degradation product (Deg I) and the alkaline hydrolysis degradation product (Deg II; formerly reported as acid metabolite). The proposed method was applied for analysis of MPZ in pure form, pharmaceutical formulation (tablets) and human plasma over the concentration range of 0.4e1.4 mg band À1 with accuracy of mean percentage recovery 100.10 AE 0.941. The method depends on separation of MPZ from its degradation products on HPTLC silica gel 60 F 254 plates using chloroformemethanoleammonia (12:2:0.1, by volume) as developing system followed by densitometric measurement of bands at 254 nm. The method was validated according to ICH guidelines. The degradation products were well resolved from the pure drug with significantly different R f values (MPZ, R f ¼ 0.58, Deg I, R f ¼ 0.35 and Deg II, R f ¼ 0.03). The results obtained by the proposed method were statistically compared to the reported HPTLC method, and there was no significant difference between them regarding both accuracy and precision; indicating the ability of the proposed method to be used for routine analysis of drug product.

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“…Impregnation was carried out by mixing l ‐Arg with the silica gel slurry (Bhushan and Parshad, ) or by immersion of plates in the solution of l ‐Arg (Sajewicz et al ., ). l ‐(−)‐Serine and l ‐(−)‐threonine have been used as chiral impregnating reagents for TLC resolution of some 2‐arylpropionic acids (Aboul‐Enien et al ., , ). Some of these results are summarized in Tables .…”

Section: Direct Resolution Using Amino Acids As Chiral Impregnating Rmentioning

confidence: 99%

“…Some of the other NSAIDs resolved into their enantiomers in a similar manner include (AE)-ibuproxam, (AE)-benoxaprofen, (AE)-ketoprofen, (AE)-pranoprofen and (AE)-tiaprofenic acid (Aboul-Enien et al, 2003b). TLC plates impregnated with L-serine or L-threonine provided separation of (AE)-ibuproxam, (AE)-ketoprofen and (AE)-pranoprofen into their enantiomers; the chiral separation factors were 1.04, 1.44 and 1.38, respectively, on plates impregnated with L-serine, while these were 1.09, 1.52 and 1.56, respectively, on plates impregnated with L-threonine.…”

Section: Amino Acids As Ion-pairing Reagents For Enantioseparationmentioning

confidence: 99%

Amino acids as chiral selectors in enantioresolution by liquid chromatography

Bhushan

Dixit

2012

Biomedical Chromatography

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Amino acids are unique in terms of their structural features and multidimensional uses. With their simple structures and the ready availability of both enantiomers, amino acids not only serve as a chiral pool for synthesis but also provide an inexpensive pool for resolution studies. There has been no attempt to review the application of amino acids as chiral selectors for chromatographic enantioresolution of pharmaceuticals and other compounds. The present paper deals with application of l-amino acids and complexes of l-amino acids with a metal ion, particularly Cu(II), as an impregnating reagent in thin-layer chromatography or as a chiral ligand exchange reagent or a chiral mobile phase additive in both thin-layer chromatography and high-performance liquid chromatography. Enantiomeric resolution of β-blockers, nonsteroidal anti-inflammatories, amino acids (and their derivatives) and certain other compounds is discussed.

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Enantiomeric resolution of some 2-arylpropionic acids using l-(−)-serine-impregnated silica as stationary phase by thin layer chromatography

Cited by 30 publications

References 11 publications

Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (±) Citalopram and its S - Enantiomer Escitalopram

Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (±) Citalopram and its S - Enantiomer Escitalopram

Stability indicating HPTLC method for determination of Metopimazine in pharmaceutical formulation and human plasma

Amino acids as chiral selectors in enantioresolution by liquid chromatography

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