Enantiomer separation of drugs by capillary electrophoresis using proteins as chiral selectors

Sensitive chiral analysis by capillary electrophoresis

“…Since enantiomeric separations are based on the differential affinity of the protein for each enantiomer, this separation mode is called affinity CE (ACE) [27]. As in the case of macrocyclic antibiotics, adequate experimental conditions have to be used in order to avoid the interaction of proteins with the wall of the capillary and to minimize the background UV absorption of protein solutions (usual concentrations are lower than 100 mM).…”

Principles, Concepts and Strategies of Stereoselective Synthesis

“…CE is applied as an efficient and versatile approach for the physicochemical characterization of natural bioactive molecules, 103,104 and chiral resolution of charged substances such as biomolecules 105,106 and low-molecularweight basic or acidic drugs. 107 Capillary electrophoresis is a powerful technique in pharmaceutical analysis, 108,109 useful in the determination of chiral purity in pharmaceuticals, and routine quality control of marketed pharmaceuticals. CE also offers the benefits of a powerful and proven technology that can be efficiently applied to drug discovery and drug development.…”

Recent advances in the study of biomolecular interactions by capillary electrophoresis

“…Many serum proteins such as BSA, HSA, serum albumin from other species, glycoproteins such as a-acid glycoprotein, crude ovomucoid, ovoglycoprotein, avidin, and riboflavin binding protein, enzymes such as fungal cellulose, cellobiohydrolase I, pepsin, and lysozyme and other proteins such as casein, human serum transferrin and ovotransferrin etc. can be used as chiral selectors in CE to separate drug enantiomers [105][106][107]. In the enantiomeric separation of ofloxacin, verapamil, and propranolol, using HSA or BSA as the buffer additive, the adsorption to the capillary wall by the protein was the key factor in the separation of ofloxacin enantiomers.…”