Enantiomer separation by pressure-supported electrochromatography using capillaries packed with Chirasil-Dex polymer-coated silica

Wistuba, Dorothee; Schurig, Volker

doi:10.1002/(sici)1522-2683(19990901)20:13<2779::aid-elps2779>3.0.co;2-f

Cited by 52 publications

(30 citation statements)

References 30 publications

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“…By applying a voltage of 20.5 kV along the 30 cm column, baseline separation is achieved for both enantiomeric pairs. Compared to the separation with UV detection (data not shown, see [29]), loss of separation efficiency through the MS interface is observed, nevertheless baseline separation is still achieved. The main advantages of coupling the CEC with the MS in the field of chiral analysis is the possibility of identification of the enantiomers also in complex mixtures and complex matrices.…”

Section: Esi-msmentioning

confidence: 92%

“…In an earlier study, the enantioselective properties of Chira-Dex-silica in pressure-supported CEC with packed capillaries was investigated [25]. Satisfactory chiral separation factors were found for derivatives of barbituric acid [25] and for herbicides based on derivatives of phenoxypropanoates [29]. Thus, these compounds were chosen as model substances for demonstrating the feasibility of pressure-supported CEC-ESI-MS and pressuresupported CEC-CIS-MS coupling.…”

Section: Cecmentioning

confidence: 99%

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On-line coupling of packed capillary electrochromatography with coordination ion spray-mass spectrometry for the separation of enantiomers

Brocke¹,

Wistuba

Gfrörer³

et al. 2002

Electrophoresis

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Pressure-supported packed capillary electrochromatography (CEC) and packed capillary high-performance liquid chromatography (pHPLC) have been coupled on-line to electrospray ionization-mass spectrometry (ESI-MS) and coordination ion spray-mass spectrometry (CIS-MS). Separation of enantiomers of barbiturates and chlorinated alkyl phenoxypropanoates were performed on a permethylated beta-cyclodextrin stationary phase by pressure-supported CEC. For on-line detection with ESI- and CIS-MS, a modified sheath-liquid interface was used. CIS-MS is a universal, novel ionization technique which improves the selectivity as well as the sensitivity. Charged complexes were formed through the addition of central complexing ions such as silver(I), cobalt(II), copper(II), and lithium(I) to the sheath flow. Advantages of CIS-MS detection compared to the ESI-MS mode are discussed. In the CIS-MS mode, increased sensitivity and high selectivity was attained through different possibilities of complexation. The superiority of pressure-supported CEC compared to pHPLC in the hyphenation with CIS-MS is demonstrated.

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Section: Esi-msmentioning

confidence: 92%

Section: Cecmentioning

confidence: 99%

On-line coupling of packed capillary electrochromatography with coordination ion spray-mass spectrometry for the separation of enantiomers

Brocke¹,

Wistuba

Gfrörer³

et al. 2002

Electrophoresis

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“…ChiraDex-beta (3mm) and ChiraDex-gamma (5 mm) were obtained from Merck (Darmstadt, Germany) and packed in fused-silica capillaries (100 mm ID; Ziemer, Mannheim, Germany) as described previously [24,25] by using an HPLC pump (Sykamp, Gilching, Germany). All pressuresupported CEC experiments were carried out with a CE system (Grom, Herrenberg, Germany) combined with a gradient pump (Sykamp, Gilching, Germany), which provided the option of pressurizing the inlet buffer vial (pressure-supported CEC).…”

Section: Methodsmentioning

confidence: 99%

Enantiomer separation by nonaqueous and aqueous capillary electrochromatography on cyclodextrin stationary phases

2001

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Native beta- and gamma-cyclodextrin bound to silica (ChiraDex-beta and ChiraDex-gamma) were packed into capillaries and used for enantiomer separation by capillary electrochromatography (CEC) under aqueous and nonaqueous conditions. Negatively charged analytes (dansyl-amino acids) were resolved into their enantiomers by nonaqueous CEC (NA-CEC). The addition of a small amount of water to the nonaqueous mobile phase enhanced the enantioselectivity but increased the elution time. The choice of the background electrolyte (BGE) determined the direction of the electroosmotic flow (EOF). With 2-(N-morpholino) ethanesulfonic acid (MES) or triethylammonium acetate (TEAA) as BGE an inverse EOF (anodic EOF) was observed while with phosphate a cathodic EOF was found. The apparent pH (pH*), the concentration of the BGE, and the nature of the mobile phase strongly influenced the elution time, the theoretical plate number and the chiral separation factor of racemic analytes.

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“…The authors showed that the same capillary can be used for CEC, capillary-HPLC and pressure supported CEC. Recently, the same group [510] reported the preparation of a polysiloxane-linked permethylb-CD thermally immobilized on silica (ChirasilDex silica 1). Recent studies by the same authors [511] deal with the comparison of open tubular CD coated capillaries and packed capillaries with CD-CSPs using both CEC and capillary-LC.…”

Section: Packed Capillariesmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

225

135

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This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

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Enantiomer separation by pressure-supported electrochromatography using capillaries packed with Chirasil-Dex polymer-coated silica

Cited by 52 publications

References 30 publications

On-line coupling of packed capillary electrochromatography with coordination ion spray-mass spectrometry for the separation of enantiomers

On-line coupling of packed capillary electrochromatography with coordination ion spray-mass spectrometry for the separation of enantiomers

Enantiomer separation by nonaqueous and aqueous capillary electrochromatography on cyclodextrin stationary phases

Chiral separation by chromatographic and electromigration techniques. A Review

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