Enantiomer separation by open-tubular liquid chromatography and electrochromatography in cellulose-coated capillaries

Chiral separation by chromatographic and electromigration techniques. A Review

“…Schurig et al [493] demonstrated the concept of unified chromatography using the same capillary coated with Chirasil-Dex for GC, capillary HPLC, SFC and CEC. Francotte and Jung [494] used capillaries coated with 3,5-dimethylphenylcarbamoyl cellulose and pmethylbenzoyl cellulose and applied the same capillaries for CEC and open tubular HPLC. The immobilization of proteins such as BSA [495] or AAG [496] via silanes directly onto the capillary wall represents a simple way of preparing open tubular capillaries.…”

Chiral Capillary Electrochromatography on a Vancomycin Stationary Phase

“…The rapid mass transfer characteristics of both phases resulted in efficiencies up to 200,000 plates per meter. Open tubular electrochromatography with cyclodextrins has been described by Schurig and coworkers [8,9] and with cellulose derivatives by Francotte and Jung [10]. To the best of our knowledge, CEC with the CSP vancomycin has not been described until now.…”

Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer

“…The previous reports of CEC enantioseparations on this kind of coated CSPs were performed under the mobile phases of acetonitrile-water or pure methanol and ethanol systems [9][10][11][12][13][14][15][16][17]. However, the typical mobile phase used for enantioseparations on polysaccharide derivative CSPs is the hexane-alcohol system according to the knowledge in HPLC [8].…”

“…Polysaccharide derivative-based chiral stationary phases (CSPs) have been shown the efficient chiral recognition ability for a variety of racemic compounds in HPLC and have been extensively studied [7,8]. Recently, polysaccharide derivatives were also applied as CSPs for CEC separation of enantiomers based on the open tubular and packed capillary columns [9][10][11][12]. For instance, capillary columns packed with cellulose tri (3,5-dimethylphenylcarbamate) and cellulose tri(3,5-dichlorophenylcarbamate) coated CSPs have been used for resolution of enantiomers by Chankvetadze et al [13][14][15][16][17], and the effect of the pore size of silica gel and the mobile phase composition on enantioseparations was systematically investigated.…”