Enamel white spot lesions can remineralise using bio-active glass and polyacrylic acid-modified bio-active glass powders

Milly, Hussam; Festy, Frederic; Watson, Timothy F.; Thompson, Ian D.; Banerjee, Avijit

doi:10.1016/j.jdent.2013.11.012

Cited by 95 publications

(91 citation statements)

References 39 publications

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“…Thereby, this study was complemented with Raman spectroscopy and cluster analysis, that offers nondestructive measures and provide an insight on biochemical nature and molecular structure, and emission spectroscopies of the tissue. It is used as a quantitative chemical assessment methodology for biological samples in conjunction with the fact that the Raman peak intensity is proportional to the number of molecules within the volume of the scanned area (Milly et al, 2014). Raman mapping, in combination with multivariate data analysis, is a label free imaging method for the analysis of dentin sections.…”

Section: Introductionmentioning

confidence: 99%

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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The aim of this study was to evaluate changes in the mechanical and chemical behavior, and bonding ability at dentin interfaces infiltrated with polymeric nanoparticles (NPs) prior to resin application. Dentin surfaces were treated with 37% phosphoric acid followed by application of an ethanol suspension of NPs, Zn-NPs or Ca-NPs followed by the application of an adhesive, Single Bond (SB). Bonded interfaces were stored for 24 h, submitted to microtensile bond strength test, and evaluated by scanning electron microscopy. After 24 h and 21 d of storage, the whole resin-dentin interface adhesive was evaluated using a Nano-DMA. Complex modulus, storage modulus and tan delta (δ) were assessed. AFM imaging and Raman analysis were performed. Bond strength was not affected by NPs infiltration. After 21 d of storage, tan δ generally decreased at Zn-NPs/resin-dentin interface, and augmented when Ca-NPs or non-doped NPs were used. When both Zn-NPs and Ca-NPs were employed, the storage modulus and complex modulus decreased, though both moduli increased at the adhesive and at peritubular dentin after Zn-NPs infiltration. The phosphate and the carbonate peaks, and carbonate substitution, augmented more at interfaces promoted with Ca-NPs than with Zn-NPs after 21 d of storage, but crystallinity did not differ at created interfaces with both ions-doped NPs. Crosslinking of collagen and the secondary structure of collagen improved with Zn-NPs resin-dentin infiltration. Ca-NPs-resin dentin infiltration produced a favorable dissipation of energy with minimal stress concentration trough the crystalline remineralized resin-dentin interface, causing minor damage at this structure.

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Section: Introductionmentioning

confidence: 99%

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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“…This increment in the relative presence of mineral, after loading SEB·P-ZnO samples, corresponded with i) an increase of 1.34 fold in the relative mineral concentration (RMC) between phosphate and phenyl (1003 cm-1 ), the aromatic ring of phenylalanine residues in collagen (Schwartz et al, 2012), and ii) an increase in crystallographic perfection in the apatite unit cell, i.e., lower FWHM, which was evidenced in Table 3A and Fig 3B. Additionally, iii) the gradient of mineral content (GMC) was lower (1.45 and 1.06 at HL and BHL, respectively), and the phosphate peaks ratio was higher (2 and 1.07 at HL and BHL, respectively) after load cycling (Table 2a). The lower carbonate substitution for phosphate, plus the higher phosphate peak ratio evokes the presence of advanced apatite maturity (Schwartz et al, 2012;Milly et al, 2014). Field emission scanning electron microscopy confirmed the presence of new mineral formation at both intertubular and peritubular dentine (Fig 5D).…”

Section: Resultsmentioning

confidence: 89%

“…Phosphate peaks ratio (PPR): it assesses the ratio between the mineral peak at 960 cm -1 (phosphate) (PO 4 3-), within the demineralised zone and the mineral peak (PO 4 3-) within the healthy substratum (Milly et al, 2014).…”

Section: Gradient In Mineral Contentmentioning

confidence: 99%

Functional and molecular structural analysis of dentine interfaces promoted by a Zn-doped self-etching adhesive and an in vitro load cycling model

Toledano

Aguilera

Osorio

et al. 2015

Journal of the Mechanical Behavior of Biomedical Materials

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The aim of this study was to evaluate if mechanical cycling influences bioactivity and bond strength at the resin-dentine interface after bonding with Zn-doped self-etching adhesives. Sound dentine surfaces were bonded with Clearfil SE Bond (Kuraray, Tokyo, Japan) (SEB), and 10 wt% ZnO microparticles (Panreac Química, Barcelona, Spain) or 2 wt% ZnCl 2 (Sigma Aldrich, St Louis, MO, USA) were added into the SEB primer (P) or bonding (Bd) for Zn-doping. Bonded interfaces were stored in simulated body fluid during 24 h, and then tested or submitted to mechanical loading. Microtensile bond strength (MTBS) was assessed for the different groups. Debonded dentine surfaces were studied by field emission scanning electron microscopy (FESEM).Remineralisation of the bonded interfaces was assessed nano-indentation, Raman spectroscopy/cluster analysis, and Masson's trichrome staining. Load cycling (LC) increased the percentage of adhesive failures in all groups. LC increased the Young's modulus (Ei) at the hybrid layer (HL) when SEB, SEB·P-ZnO and SEB·P-ZnCl 2 were applied, but decreased when both ZnO and ZnCl 2 were incorporated into the bonding (SEB·Bd). In general, Ei was higher and more uniform when Zn compounds were incorporated into the primer (SEB·P). ZnO promoted an increase, and ZnCl 2 a decrease, of both the relative presence of minerals and crystallinity, after LC. Mechanical loading increased collagen crosslinking at the interface with both SEB·P-ZnO and SEB·P-ZnCl 2 . The ratios which reflect the nature of collagen increased, in general, at both HL and BHL after LC, confirming recovery, better organization, improved structural differences and collagen quality. After loading, trichrome staining reflected a deeper demineralised dentine fringe when Zn-doped compounds were incorporated into SEB·Bd. Multiple Zn-rich phosphate deposits and salt formations were detected. 4Mineral precipitates nucleated in multilayered platforms or globular formations on peritubular and intertubular dentine.

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“…For example, Milly et al 29) showed that surface treatment with bioactive glass and polyacrylic acid enhanced the remineralization of artificial enamel white spot lesions, as demonstrated by the improved mechanical properties, higher phosphate content and morphological changes within the lesions. Also, by mixing bioglass with phosphoric acid and applying the resulting paste to eroded enamel surfaces, Bakry et al 5) were able to improve the micro-mechanical properties of the eroded enamel within a short time.…”

Section: Discussionmentioning

confidence: 99%

Remineralizing effect of cold plasma and/or bioglass on demineralized enamel

El-Wassefy

2017

Dent. Mater. J.

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This study investigated the combining effect of cold plasma and bioglass-phosphoric acid paste on demineralized enamel. Fifty bovine incisors' enamel specimens were challenged by a demineralization solution of pH 4.47 for 72 h. Specimens were divided into five groups: (I) Control, demineralized enamel (C); (II) Demineralized enamel treated with fluoride varnish (F); (III) Cold plasma application to demineralized enamel (P); (IV) Demineralized enamel treated with bioglass paste (B); (V) Application of bioglass paste to cold plasma-treated demineralized enamel (PB). Specimens were then immersed in remineralizing solution for 24 h, before being examined with micro-computed tomography (micro-CT), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and cross-sectional micro-hardness measurement. The results showed that; treating demineralized enamel with cold plasmas before bioglass application ensued a significant high mineral volume recovery and micro-hardness of demineralized region. It can be concluded that cold plasmas may improve the remineralization of bioglass on demineralized enamel.

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Enamel white spot lesions can remineralise using bio-active glass and polyacrylic acid-modified bio-active glass powders

Cited by 95 publications

References 39 publications

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Functional and molecular structural analysis of dentine interfaces promoted by a Zn-doped self-etching adhesive and an in vitro load cycling model

Remineralizing effect of cold plasma and/or bioglass on demineralized enamel

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