Enamel remineralization potential of arginine-fluoride varnish in a multi-species bacterial pH-cycling model

Bijle, Mohammed Nadeem Ahmed; Abdalla, Mohamed Mahmoud; Ashraf, Usman; Ekambaram, Manikandan; Yiu, Cynthia Kar Yung

doi:10.1016/j.jdent.2020.103528

Cited by 18 publications

(9 citation statements)

References 29 publications

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“…It also facilitates the formation of arginine-friendly microorganisms while disrupting the internal homeostasis of caries-causing bacteria ( Nascimento et al, 2019 ). The combination of Arg and fluoride can create a pH-responsive fluoride pool that inhibit acid production and has potential synergistic effects in maintaining a healthy oral microbial balance ( Agnello et al, 2017 ; Bijle et al, 2021a ). The pool can also significantly improve the fluoride uptake and surface hardness of damaged enamel compared to fluoride alone ( Zheng et al, 2015 ; Bijle et al, 2020 ).…”

Section: Functionalized Organic Materialsmentioning

confidence: 99%

Advanced materials for enamel remineralization

Shi

Luo

et al. 2022

Front. Bioeng. Biotechnol.

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Dental caries, a chronic and irreversible disease caused by caries-causing bacteria, has been listed as one of the three major human diseases to be prevented and treated. Therefore, it is critical to effectively stop the development of enamel caries. Remineralization treatment can control the progression of caries by inhibiting and reversing enamel demineralization at an early stage. In this process, functional materials guide the deposition of minerals on the damaged enamel, and the structure and hardness of the enamel are then restored. These remineralization materials have great potential for clinical application. In this review, advanced materials for enamel remineralization were briefly summarized, furthermore, an outlook on the perspective of remineralization materials were addressed.

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Section: Functionalized Organic Materialsmentioning

confidence: 99%

Advanced materials for enamel remineralization

Shi

Luo

et al. 2022

Front. Bioeng. Biotechnol.

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“…It is quite possible that prolonged treatment with higher arg concentrations led to increased arg-phosphate complex mass, thereby affecting the existent HAP crystals' orientation. Furthermore, it is explained that the higher concentrations of arg brings about a complex supersaturation over the crystal mass, leading to primary HAP-bounded arg, as the overall charge for the molecule (arg) is positive while the surface charge for the enamel is negative [41,42]. Meanwhile, at lower concentrations (i.e.,1%/2%), the prebiotic arg was assimilated into the biofilm.…”

Section: Discussionmentioning

confidence: 99%

Concentration-Dependent Multi-Potentiality of L-Arginine: Antimicrobial Effect, Hydroxyapatite Stability, and MMPs Inhibition

et al. 2021

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This study’s objective was to examine L-arginine (L-arg) supplementation’s effect on mono-species biofilm (Streptococcus mutans/Streptococcus sanguinis) growth and underlying enamel substrates. The experimental groups were 1%, 2%, and 4% arg, and 0.9% NaCl was used as the vehicle control. Sterilised enamel blocks were subjected to 7-day treatment with test solutions and S. mutans/S. sanguinis inoculum in BHI. Post-treatment, the treated biofilms stained for live/dead bacterial cells were analysed using confocal microscopy. The enamel specimens were analysed using X-ray diffraction crystallography (XRD), Raman spectroscopy (RS), and transmission electron microscopy (TEM). The molecular interactions between arg and MMP-2/MMP-9 were determined by computational molecular docking and MMP assays. With increasing arg concentrations, bacterial survival significantly decreased (p < 0.05). The XRD peak intensity with 1%/2% arg was significantly higher than with 4% arg and the control (p < 0.05). The bands associated with the mineral phase by RS were significantly accentuated in the 1%/2% arg specimens compared to in other groups (p < 0.05). The TEM analysis revealed that 4% arg exhibited an ill-defined shape of enamel crystals. Docking of arg molecules to MMPs appears feasible, with arg inhibiting MMP-2/MMP-9 (p < 0.05). L-arginine supplementation has an antimicrobial effect on mono-species biofilm. L-arginine treatment at lower (1%/2%) concentrations exhibits enamel hydroxyapatite stability, while the molecule has the potential to inhibit MMP-2/MMP-9.

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“…It has been suggested that arginine be incorporated into fluoride products (particularly a mixture of 2% Larginine with 5% NaF varnish) to improve physical properties, enhance fluoride uptake and remineralization potential, and render a stable matrix with higher fluoride/ arginine long-term release compared with controls. 511,512…”

Section: Silver and Silver Diamine Fluoridementioning

confidence: 99%