ENaC- and CFTR-dependent ion and fluid transport in human middle ear epithelial cells

Choi, Jae Young; Son, Eun Ju; Kim, Jung Lim; Lee, Joo-Hyeung; Park, Hun Yi; Kim, Sung Huhn; Song, Mee Hyun; Yoon, Joo Heon

doi:10.1016/j.heares.2005.08.007

Cited by 22 publications

(25 citation statements)

References 27 publications

(34 reference statements)

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“…Fluid absorption is the consequence of an osmotic gradient driven by Na + transport, responsible for the transepithelial lumen-negative potential difference. The transport of Na + generated a passive Cl -absorption through the paracellular pathway, which generates a major transepithelial osmotic gradient [34,35] .…”

Section: Discussionmentioning

confidence: 99%

Expression Pattern of Aquaporin 1 in the Middle Ear of the Guinea Pig with Secretory Otitis Media

Zhang

Liu²,

Gao³

et al. 2008

ORL

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Objective: To explore the pathological change of water homeostasis in the secretory otitis media (SOM) middle ear, we observed the expression and regulation of aquaporin 1 (AQP1) in the SOM middle ear cavity. Methods: Reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and immunohistochemical staining were used to detect AQP1 in the bullae of SOM models and normal animals. The expression patterns of AQP1 in the SOM group were compared with those in the normal animal group. Results: RT-PCR and immunoblot analyses revealed that mRNAs encoding AQP1 were expressed in the middle ear membrane of the guinea pigs of both groups; AQP1 was also detected as 28-kDa proteins in both groups. Immunohistochemical analysis showed that AQP1 was localized on capillary endothelial cells and fibroblasts in the lamina propria mucosae as well as on flat and cubical epithelial cells. Quantitative analysis of RT-PCR and Western blotting revealed that AQP1 expression was higher in the SOM group than in the control group. The cellular expression of AQP1 was somewhat altered in the SOM middle ear. Conclusions: Our study suggests that AQP1 in the middle ear cavity may play a vital role in the accumulation of the effusion. It might work on the vessel-caused hydrops in the middle ear cavity.

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Section: Discussionmentioning

confidence: 99%

Expression Pattern of Aquaporin 1 in the Middle Ear of the Guinea Pig with Secretory Otitis Media

Zhang

Liu²,

Gao³

et al. 2008

ORL

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“…The majority of current was decreased by amiloride analogues where the order of potency was benzamil Ͼ amiloride Ͼ Ͼ EIPA, which suggests that transepithelial Na ϩ transport via ENaC mostly contributes to the current. Some Na ϩ -absorbing epithelia, including semicircular canal duct, are capable of bidirectional salt transport and secrete Cl Ϫ under stimulation of the cAMP signaling pathway (Mall et al, 1999;Chan et al, 2000;Kunzelmann et al, 2000;Milhaud et al, 2002;Choi et al, 2006). In contrast, no immediate response of current to a mixture known to raise intracellular cAMP levels was observed from the saccule.…”

Section: Namentioning

confidence: 99%

Endolymphatic Sodium Homeostasis by Extramacular Epithelium of the Saccule

Kim

Marcus²

2009

J. Neurosci.

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The saccule is a vestibular sensory organ that depends upon regulation of its luminal fluid, endolymph, for normal transduction of linear acceleration into afferent neural transmission. Previous studies suggested that endolymph in the saccule was merely derived from cochlear endolymph. We developed and used a preparation of isolated mouse saccule to measure transepithelial currents from the extramacular epithelium with a current density probe. The direction and pharmacology of transepithelial current was consistent with Na ϩ absorption by the epithelial Na ϩ channel (ENaC) and was blocked by the ENaC-specific inhibitors benzamil and amiloride. Involvement of Na

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“…Outra possível explicação é que pode haver diferentes expressões genéticas das mucinas no ouvido médio e na mucosa da árvore brônquica, o que pode contribuir para uma menor produção de muco nesses pacientes 7,8 . Além disso, Todd & Martin 9 supõem que o gene responsável pela formação correta da tuba auditiva está relacionado ao gene que determina a FC, e Choi et al 10 relataram que, na mucosa normal do ouvido médio, a expressão do regulador de condutância transmembrana em fibrose cística (CFTR) parece ser menos proeminente do que na mucosa nasal, e um canal de cloro induzido por ATP pode ser uma alternativa ao CFTR. Devido a essas particularidades, a manutenção do ouvido médio sem fluídos pode ser menos dependente do CFTR, e portanto menos alterada em pacientes com FC.…”

Section: Discussionunclassified

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Martins

Guimarães²,

Becker³

et al. 2011

J Pediatr (Rio J)

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Objective: To assess the prevalence of middle ear alterations in cystic fibrosis (CF) patients. Methods:In this descriptive study, 120 CF patients aged 5 months to 18 years were assessed by clinical history, otoscopy, and tympanometry. Data on P. aeruginosa colonization and parenteral and/or inhaled aminoglycoside use were also collected from medical charts. Results:Clinical history revealed absence of previous otitis media in 57% of patients. Tympanic membranes were normal in 94% of patients who underwent otoscopic evaluation; chronic otitis media was suggested in about 1% of the cases, otitis media with effusion (OME) in 2%, and Eustachian tube dysfunction in 3%. As for tympanometry, 91% of patients who underwent the exam showed normal results, OME was suggested in 2% of the cases, and Eustachian tube dysfunction in 7%. Conclusion:There was a low prevalence rate of middle ear alterations in our series of CF patients. The use of aminoglycosides and colonization by P. aeruginosa did not influence the prevalence of middle ear alterations. Our results suggest that a detailed clinical history and a routine otoscopy evaluation may confirm or rule out most middle ear alterations in CF patients.J Pediatr (Rio J). 2011;87(1):80-83: Cystic fibrosis, middle ear, tympanometry, tympanoscopy. ResumoObjetivo: Avaliar a prevalência de alterações do ouvido médio em pacientes com fibrose cística (FC). Métodos:Neste estudo descritivo, 120 pacientes com FC com idade entre 5 meses e 18 anos foram avaliados por meio do histórico clínico, da otoscopia e da timpanometria. Dados sobre a colonização por P. aeruginosa e uso de aminoglicosídeos por via parenteral e/ou inalatória também foram coletados a partir dos prontuários médicos.Resultados: O histórico clínico revelou ausência de otite média pré-via em 57% dos pacientes. As membranas timpânicas estavam normais em 94% dos pacientes que se submeteram a avaliação otoscópica; foi sugerida otite média crônica em cerca de 1% dos casos, otite média com derrame (OMD) em 2%, e disfunção da tuba auditiva em 3%. Quanto à timpanometria, 91% dos pacientes que se submeteram ao exame apresentaram resultados normais, foi sugerida OMD em 2% dos casos, e disfunção da tuba auditiva em 7%. Conclusão:Houve uma baixa taxa de prevalência de alterações do ouvido médio na nossa série de pacientes com FC. O uso de aminoglicosídeos e a colonização por P. aeruginosa não tiveram influência na prevalência das alterações do ouvido médio. Nossos resultados sugerem que um histórico clínico detalhado e uma avaliação otoscópica de rotina podem confirmar ou descartar a maior parte das alterações do ouvido médio em pacientes com FC.

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ENaC- and CFTR-dependent ion and fluid transport in human middle ear epithelial cells

Cited by 22 publications

References 27 publications

Expression Pattern of Aquaporin 1 in the Middle Ear of the Guinea Pig with Secretory Otitis Media

Expression Pattern of Aquaporin 1 in the Middle Ear of the Guinea Pig with Secretory Otitis Media

Endolymphatic Sodium Homeostasis by Extramacular Epithelium of the Saccule

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