Enabling technologies to improve area‐wide integrated pest management programmes for the control of screwworms

Robinson, A. S.; Vreysen, Marc J. B.; Hendrichs, Jorge; Feldmann, U.

doi:10.1111/j.1365-2915.2008.00769.x

Cited by 34 publications

(30 citation statements)

References 30 publications

(35 reference statements)

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“…For SIT effectiveness, to assure the mating between wild females and the released sterile insects is much more critical than only knowing the genotype of the insects (Robinson et al 2009). So far, there is not sufÞcient, unequivocal evidence through genetic analyses to contest the hypothesis that C. hominivorax is a single, panmitic species throughout both its historical and existing ranges.…”

Section: Discussionmentioning

confidence: 99%

Feasibility of Using a Caribbean Screwworm for SIT Campaigns in Brazil

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Feasibility of Using a Caribbean Screwworm for SIT Campaigns in Brazil

et al. 2012

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“…have shown difficulties in the morphological differentiation of C. macellaria and C. hominivorax (unpublished data). Griffiths et al (2009), Torres and Azeredo-Espin (2009), Robinson et al (2009) and Lyra et al (2009) have reported the use of different molecular techniques and several modifications of the DNA isolation protocols for Cochliomyia spp. The principal changes were based on the type of sample (legs, abdomen and complete specimen) to be analyzed and focused to phenolchloroform or commercial kit methods, but the quantification and purity was not reported.…”

Section: Introductionmentioning

confidence: 99%

“…The annual losses caused by screwworm infestations in the United States, Mexico and Central America were significant; while in some Caribbean islands and South America it remains as a latent problem (Forero et al, 2007;Rodríguez et al, 2011). The programs based on the sterile insect technique eradicated the NWS caused by C. hominivorax from the southern United States, Mexico, Central America and some Caribbean islands (Robinson et al, 2009). For the period 2012-2021 the FAO has provided strategies for collecting information about the incidence of populations of screwworm and the impact of NWS on public health, in order to establish the eradication program in South America (FAO, 2011).…”

Section: Introductionmentioning

confidence: 99%

A new DNA extraction protocol for screwworm fly Cochliomyia species (Diptera: Calliphoridae)

Echeverría-Fonseca

Mera-Ruiz

Carrillo-Toro

et al. 2015

Front. Environ. Sci.

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Modifications to the DNA isolation protocol from Cochliomyia spp., are suggested based on the Chelex® 100 reactive. To apply the sterile insect technique (SIT) program it is necessary to study the molecular variations of endemic populations with efficient, fast and low costs techniques. The test samples were collected in the Pichincha province of Ecuador. The isolation protocol had 3 steps: (a) pretreatment (optional), (b) mechanic and chemical lysis, (c) two incubations; then the supernatant were separated by centrifugation. Furthermore, variations in concentrations of magnesium chloride present in the master mix were evaluated. Results showed a high efficiency in isolation with approximately 1.20 h of manipulation (without pretreatment). Additionally, the quality of the amplicon that was visualized on 2% agarose (w/v) showed that the magnesium chloride concentration was influential in the PCR reaction mix.

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“…It produces severe cutaneous lesions in mammals, including domestic and wild animals and human beings (Guimarães & Papavero 1999). This fly was originally distributed throughout the southwestern USA and the Americas and has been eradicated from the USA, Mexico and part of Central America using the sterile insect technique (Galvin & Wyss 1996, Robinson et al 2009). In Brazil, this species is widespread and can be found in all states of the country.…”

Section: Introductionmentioning

confidence: 99%

Proteolytic activity of excretory/secretory products of Cochliomyia hominivorax larvae (Diptera: Calliphoridae)

et al. 2016

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The protein profiles and proteolytic activity of the excretory secretory products (E/SP) of the first (L1), second (L2) and third (L3) larval stages of Cochliomyia hominivorax were studied in the laboratory. Analysis on the E/SP protein profile was carried out using polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). The E/SP of each larval stage (L1, L2 and L3) treated with protease inhibitors, containing 30μg, 40μg and 50μg of protein, was applied to the 10% polyacrylamide gel. The proteolytic activity of the crude E/SP was analyzed in gels copolymerized with gelatin and by colorimetric assays using azocasein as a substrate, with the characterization of the proteases using synthetic inhibitors. Different protein profiles were observed for the larval instars, with L1 presenting the most complex profile. Nevertheless, various protein bands were observed that were common to all the larval instars. The E/SP of all the instars showed proteolytic activity on gelatin, evidenced by proteolysis zones, predominantly with apparently higher molecular masses in L1, while for L2 and L3 the proteolysis zones could also be observed in regions with lower masses. Tests with protease inhibitors using gelatin as substrate showed that the E/SP of larvae were mainly composed of serine proteases. Additionally, inhibition was observed in L2 E/SP treated previously with EDTA, an inhibitor of metalloproteases. The assays with azocasein revealed a gradual increase of proteolytic activity on this substrate with larval development progress, with the strongest inhibitions being observed after treatments with 3,4-dichloroisocoumarin (DCI) for E/SP of L1, L2 and L3. These results suggest that C. hominivorax larvae produce different proteases, a fact that can be related to the parasite's vital processes for survival, such as penetration into the host's tissues and nutrition during the larval stage.

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Enabling technologies to improve area‐wide integrated pest management programmes for the control of screwworms

Abstract: . The economic devastation caused in the past by the New World screwworm fly Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) to the livestock industry in the U.S.A., Mexico and the rest of Central America was staggering.

Cited by 34 publications

References 30 publications

Feasibility of Using a Caribbean Screwworm for SIT Campaigns in Brazil

Feasibility of Using a Caribbean Screwworm for SIT Campaigns in Brazil

A new DNA extraction protocol for screwworm fly Cochliomyia species (Diptera: Calliphoridae)

Proteolytic activity of excretory/secretory products of Cochliomyia hominivorax larvae (Diptera: Calliphoridae)

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