Enabling bone formation in the aged skeleton via rest-inserted mechanical loading

Osteogenic Effects of Rest Inserted and Continuous Cyclic Tensile Strain on hASC Lines with Disparate Osteodifferentiation Capabilities

“…Although our results do not correspond with rest insertion studies using bone marrow-derived MSCs, bone precursor cells, or osteoblasts, 45,51,53 it should be noted that most of those studies were performed in vivo where other chemical stimuli may have been present or in vitro using different cell types. Human ASCs have already been reported to differ from other mesenchymal cells with respect to differentiation potential, 10,20 nutritional requirements, 14 and surface marker profile.…”

“…57 In an effort to restore mechanosensitivity in cells and therefore optimize the osteogenic effects of mechanical loading, researchers have incorporated rest periods between loading cycles. The effects of long (hours) and short (seconds) recovery periods have been investigated both in vivo 26,44,45,[51][52][53] and in vitro, 4,12 and the existence of a recovery threshold has been demonstrated. 45 Robling et al found that when evaluating 0.5, 3.5, 7, and 14 s of rest between mechanical loading cycles on rat tibiae, improvements in relative mineralized surface and rate of bone formation were significantly greater when 14 s of rest were applied between loading cycles.…”

Lifestyle factors, medications, and disease influence bone mineral density in older men: findings from the CHAMP study

“…However, it is unclear how age affects the osteogenic response in older men. Some [32][33][34], though MCI mild cognitive impairment not all [35], animal studies have found that bone cells of older animals are less responsive to forces than corresponding cells in young animals. It is possible that our finding reflects the decreased responsiveness of older bone to loading.…”

A Finite Element Dual Porosity Approach to Model Deformation-Induced Fluid Flow in Cortical Bone

“…The theory of dual porosity, developed in the 70s by Duguid 10,11,12 and Aifantis 1,2 to simulate the flow through fissured porous media, is here used to predict the movement of fluid within cortical bone at two levels of porosity (PV and PLC). Two different models have been developed to test the potential of the dual porosity applied to bone: a 2D model of a small sample of cortical bone, reproducing the simulation of Starkebaum et al 25 and a 3D rat tibia model reproducing the experiments of Srinivasan et al 24 The ability of this approach to reproduce complex bone behavior and its potential to be used in bone remodeling studies are discussed according to the results obtained with our models.…”

“…Next, we simulate the experiments performed by Srinivasan et al 24 in order to compare the influence of using a simple or dual porosity model in the fluid flow of a whole bone. For this objective, a finite element mesh of the cortical bone of a rat tibia was developed.…”