Emulsion-templated synthetic polypeptide scaffolds prepared by ring-opening polymerization ofN-carboxyanhydrides

Abstract

<p>Ring-opening polymerization of <italic>N</italic>-carboxyanhydrides was performed in oil-in-oil high internal phase emulsion to obtain well-defined macroporous synthetic polypeptides.</p>

Preparation of Synthetic Polypeptide–PolyHIPE Hydrogels with Stimuli-Responsive Behavior

“…In our recent study, we developed a method for preparation of polypeptide− polyHIPE organogels via ROP of NCA monomers in oil-in-oil emulsions. 44 Using this approach, we have here prepared redox-responsive PBLG as well as P(BLG-co-Phe) and P(BLGco-ZLL) copolypeptide polyHIPEs using equimolar amounts of L-phenylalanine(Phe)-or L-lysine(Lys)-based monomers to study the effect of incorporating hydrophobic or amine groups into the hydrogel structure on their stimuli-responsive behavior. A di-NCA derivative of L-cystine containing a disulfide bond in the structure was used as a cross-linker.…”

“…Due to the sensitivity of the ROP of NCA to the presence of water, we chose an organogel-to-hydrogel route to prepare polypeptide–polyHIPE hydrogels (Figure ). In our recent study, we developed a method for preparation of polypeptide–polyHIPE organogels via ROP of NCA monomers in oil-in-oil emulsions . Using this approach, we have here prepared redox-responsive PBLG as well as P­(BLG- co -Phe) and P­(BLG- co -ZLL) copolypeptide polyHIPEs using equimolar amounts of l -phenylalanine­(Phe)- or l -lysine­(Lys)-based monomers to study the effect of incorporating hydrophobic or amine groups into the hydrogel structure on their stimuli-responsive behavior.…”

“…Recently, we reported a synthetic strategy to combine NCA chemistry and HIPE templating to prepare hydrophobic polyHIPE organogels based on synthetic polypeptides . In this work, we report the preparation of polyHIPE hydrogels composed entirely of natural amino acids by an organogel-to-hydrogel route.…”

“…42,43 Recently, we reported a synthetic strategy to combine NCA chemistry and HIPE templating to prepare hydrophobic polyHIPE organogels based on synthetic polypeptides. 44 In this work, we report the preparation of polyHIPE hydrogels composed entirely of natural amino acids by an organogel-tohydrogel route. Biodegradable stimuli-responsive polyHIPE hydrogels were prepared from Glu cross-linked with L-cystine and Glu copolymerized with equimolar amounts of L-lysine (Lys) or L-phenylalanine (Phe) to modulate the physicochemical properties of the hydrogels, such as buffer uptake, compressive properties, and in vitro enzymatic degradation rates.…”

Self-assembled silk fibroin hydrogels: from preparation to biomedical applications

“…However, the survival was lower in the gels with higher concentrations, likely due to limitations in the mass transport due to decreased mesh size of the hydrogel. 124 SF hydrogels produced via this method are popular biomaterials for bone and cartilage tissue engineering applications. Ding et al used fibroin hydrogels as scaffolds to deliver rat bone marrow stem cells into bone defects.…”