Emulsion-templated microparticles with tunable stiffness and topology: Applications as edible microcarriers for cultured meat

Norris, Sam C. P.; Kawecki, Stephanie; Davis, Ashton; Chen, Kathleen K; Rowat, Amy C.

doi:10.1016/j.biomaterials.2022.121669

Cited by 39 publications

(21 citation statements)

References 108 publications

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Section: Resultsmentioning

confidence: 99%

“…[38,42] Our results suggest that Ca 2+ release from bioglass nanoparticles can be used to provide mechanical cues for mechanosensitive cells, such as myoblasts, to promote their differentiation into myotubes. [43][44][45][46] Of note, we also conducted experiments analogous to those with added bioglass but instead using silica nanoparticles at the same concentrations. The silica nanoparticles have a similar size and morphology to those of the bioglass particles that we used in our experiments.…”

Section: Production Of Axial Gradientsmentioning

confidence: 99%

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Plug‐and‐Play Multimaterial Chaotic Printing/Bioprinting to Produce Radial and Axial Micropatterns in Hydrogel Filaments

Ceballos‐González,

Bolívar‐Monsalve,

Quevedo‐Moreno

et al. 2023

Adv Materials Technologies

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Nature abounds with micro‐architected materials containing layered multi‐material patterns that often transition within the very same monolithic piece. Fabricating these complex materials using current technologies is challenging. Multimaterial chaotic printing is presented—an extrusive printing method based on the use of chaotic advection—that can fabricate microstructured hydrogels with well‐defined multimaterial and multilayered micropatterns. Printheads containing internal Kenics static mixing (KSM) elements and top‐ and lateral‐positioned inlets are used to produce a wide repertoire of multilayered hydrogel filaments. In this plug‐and‐play system, the radial and axial micropatterns can be designed ad hoc by defining the printhead configuration (i.e., the number of KSM elements and inlets, and the inlet positions) and the flow program (i.e., activation/deactivation of the ink‐flow through each inlet). Computational fluid dynamics simulations closely predict the microstructure obtained by a given printhead configuration. The application of this platform is illustrated for easy fabrication of fibers with radial microgradients, bacterial ecosystems, structured emulsions, micro‐channeled hydrogel filaments, a pre‐vascularized tumor niche model, and skeletal muscle‐like tissues with axial and radial transitions of bioactive glass compartments. It is envisioned that multimaterial chaotic printing will be a valuable addition to the toolbox of additive manufacturing for the rational fabrication of advanced materials.

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Section: Resultsmentioning

confidence: 99%

Section: Production Of Axial Gradientsmentioning

confidence: 99%

Plug‐and‐Play Multimaterial Chaotic Printing/Bioprinting to Produce Radial and Axial Micropatterns in Hydrogel Filaments

Ceballos‐González,

Bolívar‐Monsalve,

Quevedo‐Moreno

et al. 2023

Adv Materials Technologies

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“…Indeed, conjugating cell adhesion motifs to the polymer backbone is not always required, especially when the scaffold is made from animal-derived materials, such as collagen and gelatin. In some cases, nonmodified alginate scaffolds have supported cell adhesion in short-term culture conditions [ 30 , 31 , 32 ]; however, cell adhesion on nonmodified alginate was significantly lower compared to gelatin [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Dual-Crosslinked Alginate-Based Hydrogels with Tunable Mechanical Properties for Cultured Meat

Tahir

Floreani

2022

Foods

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Cultured meat refers to the production of animal tissue by utilizing the same techniques as tissue engineering through cell culture. Various biomaterials have been designed to serve as in vitro supports for cell viability, growth, and migration. In this study, visible light and dual-crosslinked alginate hydrogels were designed to enable control of the physical and mechanical properties needed for the fabrication of cultured meat scaffolds. We hypothesized that a difference in hydrogel stiffness would influence cell behavior, indicating the efficacy of our processing methods to benefit the cultured meat field. Herein, we synthesized and created: (1) methacrylated alginate (AlgMA) to enable covalent crosslinking via visible light exposure, (2) Methacrylated alginate and arginyl-glycyl-aspartic acid RGD conjugates (AlgMA-RGD), using carbodiimide chemistries to provide cell-binding sites on the material, and (3) designer hydrogels incorporating different crosslinking techniques. The material and mechanical properties were evaluated to determine the structural integrity of the hydrogels, and in vitro cell assays were conducted to verify cytocompatibility and cell adhesion. Gelation, swell ratio, and weight loss calculations revealed longer gelation times for the AlgMA scaffolds and similar physical properties for all hydrogel groups. We showed that by adjusting the polymer concentration and the crosslinking methodology, the scaffold’s mechanical properties can be controlled and optimized within physiological ranges. Incorporating dual crosslinking significantly increased the compressive moduli of the AlgMA hydrogels, compared to visible-light crosslinking alone. Moreover, the muscle satellite cells responded favorably to the AlgMA scaffolds, with clear differences in cell density when cultured on materials with significantly different mechanical properties. Our results indicate the usefulness of the dual-crosslinking alginate hydrogel system to support in vitro meat growth.

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“…Substrate stiffness of approximately 8-16 kPa has been found optimal for myogenesis in certain cell types (Engler et al, 2004). Microcarriers should have a diameter of at least 100 lm, and larger microcarriers promote better cell attachment, while smaller ones result in higher growth rates due to increased shear stress (Norris et al, 2022). The review article by Bomkamp et al extensively examines the estimated requirements for suitable scaffolds in CM production.…”

Section: Requirements Of Scaffolds and Microcarriersmentioning

confidence: 99%

“…4b; they produced gelatin fibres with a diameter similar to the collagen fibres' diameter (MacQueen et al, 2019). Norris et al (2022) developed microcarriers by utilising waterin-oil emulsions as templates for gelatin microparticles by applying an embossing technique to create a grooved surface texture on the microcarriers, which showed that both smooth and grooved edible microcarriers effectively facilitated the growth and differentiation of C2C12 in suspension culture. Additionally, Liu et al employed a 3D porous gelatin microcarrier in two spinner flask bioreactors, one for muscle and the other for fat.…”

Section: Animal-based Protein Materialsmentioning

confidence: 99%

Review in edible materials for sustainable cultured meat: scaffolds and microcarriers production

Moslemy,

Sharifi,

Asadi‐Eydivand

et al. 2023

Int J of Food Sci Tech

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SummaryCultured meat, also known as lab‐grown or cell‐based meat, is a promising concept in the food industry that involves culturing animal cells in vitro to produce meat products, bypassing the need for traditional animal rearing and slaughter. This technology offers solutions to key challenges associated with conventional meat production, including environmental sustainability, animal welfare, and food security. However, the cultured meat industry must overcome significant hurdles to achieve commercial viability. Scaling up production to meet the increasing demand for meat is a considerable challenge, requiring a scalable edible scaffold, eliminating the need for separate scaffold removal, and ensuring product safety. Various edible materials, including polysaccharides and proteins, along with fabrication techniques like electrospinning and 3D printing, are explored in this review article.

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Emulsion-templated microparticles with tunable stiffness and topology: Applications as edible microcarriers for cultured meat

Cited by 39 publications

References 108 publications

Plug‐and‐Play Multimaterial Chaotic Printing/Bioprinting to Produce Radial and Axial Micropatterns in Hydrogel Filaments

Plug‐and‐Play Multimaterial Chaotic Printing/Bioprinting to Produce Radial and Axial Micropatterns in Hydrogel Filaments

Dual-Crosslinked Alginate-Based Hydrogels with Tunable Mechanical Properties for Cultured Meat

Review in edible materials for sustainable cultured meat: scaffolds and microcarriers production

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