Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation

Abstract

Microencapsulation with biodegradable polymers has potential application in drug and cell delivery systems and is currently used in probiotic delivery. In the present study, microcapsules of human fibroblast cells (CRL2522) were prepared by emulsion cross-linking technique. Tween 80 surfactant at a 2% concentration through phase inversion resulted in the most efficient and stable size, morphology, and the cells survival at least 50% on day 14. Emulsion cross-linking microcapsule preparation resulted in smaller and possibly more diverse particles that can be developed clinically to deliver encapsulated mammalian cells for future disease treatments.

Microencapsulation of <i>Ruellia tuberosa</i> L. Extracts Using Alginate: Preparation, Biological Activities, and Release

“…Microencapsulation is the shielding process of small particles using a polymer. This method resulted in microcapsules with a size range of 2-2000 μm [9][10]. The primary purpose of microencapsulation is to avoid the degradation or inactivation of the active compounds due to the unstable environmental conditions [9].…”

Magnetically‐actuated microcages for cells entrapment, fabricated by laser direct writing via two photon polymerization

“…Cells entrapment techniques include physical encapsulation in polymeric beads, such as microgels ( Zhou et al, 2018 ; Veernala et al, 2021 ) or alginate beads ( Shao et al, 2020 ; Hasturk et al, 2022 ), penetration and attachment of cells into porous 3D scaffolds ( Wu et al, 2020 ; Czosseck et al, 2022 ) or fiber‐based matrices ( Matera et al, 2019 ; Davidson et al, 2021 ; Sahu et al, 2021 ), bioreactors based on porous membranes ( Skrzypek et al, 2017 ; Bose et al, 2020 ), films made of super‐adhesive materials ( Suneetha et al, 2019 ; Nagano et al, 2021 ) and antibody‐conjugated magnetic beads ( Xu H et al, 2011 ; Nath et al, 2015 ). These devices can be obtained using innovative technologies such as 3D printing ( Agarwal et al, 2020 ; Dey and Ozbolat, 2020 ), photolithography ( Tricinci et al, 2015 ; Larramendy et al, 2019 ; Tenje et al, 2020 ), electrospinning ( Canbolat et al, 2011 ; Zussman, 2011 ; Ang et al, 2014 ), emulsion methods to obtain polymeric droplets ( López et al, 1997 ; Chaemsawang et al, 2018 ; Qu et al, 2021 ), surface coating technologies ( Yoo et al, 2011 ), sol‐gel encapsulation ( Kamanina et al, 2022 ), template‐assisted techniques ( Khademhosseini et al, 2006 ), etc. The cells are kept inside the device through physical immobilization ( Zhou et al, 2018 ; Shao et al, 2020 ; Veernala et al, 2021 ; Hasturk et al, 2022 ), extracellular‐matrix‐like adherence ( Rao and Winter 2009 ), specific antigen‐antibody recognition ( Roupioz et al, 2011 ; Boulanger et al, 2022 ), barrier containing ( Spagnolo et al, 2015 ; Larramendy et al, 2019 ; Li et al, 2021b ) and external stimuli‐activated entrapment ( Fu et al, 2008 ; Long et al, 2020 ), etc.…”

Effect of Okra Fruit (Abelmoschus esculentus) Extract on Adenocarcinoma Mammae by Assessing Caspase-3 Expression and Apoptosis Index: An In Vivo Study

“…The high content of flavonoids, isoquercetin, quercetin-3-O-gentiobiose, lectins, and pectins in okra extract is often studied for its anti-cancer benefits. [9][10][11][12][13] This study aimed to assess the effect of okra fruit extract on the apoptosis response to adriamycin-cyclophosphamide chemotherapy in vivo.…”