Emulsifying properties of canola and flaxseed protein isolates produced by isoelectric precipitation and salt extraction

Effect of maleylation on physicochemical and functional properties of rapeseed protein isolate

“…The 568 nm laser line was used for excitation, inducing a fluorescent emission of Rhodamine B (Arancibia et al 2015), detected between 600 and 700 nm (Vasbinder et al 2003). Average droplet size (μm) was measured by ImageJ® software and represented by d 32 (Karaca et al 2011).…”

“…Reduced availability of free thiol groups or the presence of negative maleyl groups in modified proteins probably gave rise to fewer disulfide bonds, as opposed to that of the control. Oilseed protein isolates are known for their high S 0 (Karaca et al 2011). S 0 increased progressively up to 0.4MA, but decreased thereafter (Fig.…”

Food Wastes as Valuable Sources of Bioactive Molecules

“…References Proteins Cereals Brewers' spent grain Ultrasonic-assisted extraction [17] Sequential extraction of proteins and arabinoxylans [18] Enzymatic-assisted extraction [19] Oil crops Rapeseed meal Ultrasound-assisted aqueous extraction [20] Sunflower meals Alkaline solubilization and acid precipitation [21] Hazelnuts meal Solvent extraction (water, acetone) [22] Canola meals Alkaline solubilization and acid precipitation (Isoelectric precipitation) [23,24] Electro-activated solutions (non-invasive extraction method) [25] Salt precipitation [24] Palm kernel cake Enzymatic hydrolysis [26] Fruits and vegetable Apricot kernel cake Alkaline solubilization and acid precipitation [27] Polysaccharides Cereals Brewers' spent grain Enzymatic hydrolysis [28] Sequential extraction of proteins and arabinoxylans [18] Acid hydrolysis [29] Fruits and vegetables Citrus peel and apple pomace Subcritical water extraction [30] Orange peel Microwave extraction [31] Lipids Cereals Brewers' spent grain Soxhlet extraction [32] Fruit and vegetables Grape seeds Pressurized carbon dioxide extraction with compressed carbon dioxide as solvent and ethanol as co-solvent [33] Supercritical fluid extraction [34] Polyphenols Cereals Brewers' spent grain Alkaline hydrolysis [35] Oil crops Rapeseed Ultrasound-assisted aqueous extraction [20] Olive by-products Continuous counter-current liquid-liquid extraction [36] Chemical (acid) hydrolysis [37] …”

“…These seeds contain two main types of storage proteins: salt-soluble (cruciferin) and water-soluble (napin), the total protein content in the defatted canola meal being around 32% [24]. The concentration of proteins in canola protein isolates, when conventional direct alkaline extraction is used, ranged between 66% and 76% [23,24], while using salt precipitation method may increase the concentration of proteins in isolates up to 93% [24].…”

“…These seeds contain two main types of storage proteins: salt-soluble (cruciferin) and water-soluble (napin), the total protein content in the defatted canola meal being around 32% [24]. The concentration of proteins in canola protein isolates, when conventional direct alkaline extraction is used, ranged between 66% and 76% [23,24], while using salt precipitation method may increase the concentration of proteins in isolates up to 93% [24]. There are new emerging non-invasive methods, such as electroactivated solutions, that can be used for the extraction of proteins from canola meals with better extraction yields by solubilising the proteins without damaging their native conformations and maintaining their functional properties [25].…”