Emulsification of hydrocarbons by subsurface bacteria

Francy, Donna S.; Thomas, John; Raymond, R. L.; Ward, C. H.

doi:10.1007/bf01576061

Cited by 95 publications

(60 citation statements)

References 8 publications

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“…1 mL of each soil sample was added to 9 ml of 0.9 % sodium chloride solution. The mixture was placed on a reciprocal shaker for 1 h. to produce a well dispersed suspension (Francy and Thomas, 1991;Adria et al, 2003). By inoculating sheep blood agar, heamolytic test was performed (Banat, 1995).…”

Section: Methodsmentioning

confidence: 99%

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Ability of indigenous Bacillus licheniformis and Bacillus subtilis in microbial enhanced oil recovery

Haghighat

Sepahy

Assadi

et al. 2008

Int. J. Environ. Sci. Technol.

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ABSTARCT: Microbially produced lipopeptide have been isolated and studied for microbial enhanced oil recovery.About 60 gram positive bacteria isolated from soil contaminated with crude oil, near the crude oil storage tank in Tehran Refinery, Tehran, Iran. However, most of these studies have produced lipopeptide by one of the pure-culture microbes isolated in a laboratory. Among the isolates, heamolytic tests revealed two biosurfactant producers. The isolated strains were designated as C2, E1. By using morphological, biochemical and molecular biology tests (16 SrRNA), the strains identified as Bacillus licheniformis and Bacillus subtitlis, respectively. Emulsification activity and measurement of surface tension indicated that, the isolates were high producers of biosurfactant. The product of C2 and E1 is mainly lipopeptide. This product reduce surface tension from 65 to 30 mN/m. Emulsified activity of crude oil was 92% for C2 and 90 % in case of E1. This is the first report of indigenous Bacillus licheniformis and Bacillus subtilis from a soil contaminated with oil in an Iranian refinery with ability to produce biosurfactant.

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Section: Methodsmentioning

confidence: 99%

“…The cultures were grown in this broth for 8-12 at 35 °C (OD 450 nm 0.8-0.9). For biosurfactant synthesis a mineral salts medium (MSM) with the following composition was used: Na 2 HPO 4 2 g/L, (Francy and Thomas, 1991). The carbohydrate (glucose, sucrose, fructose and mannose) were added to a final concentration of 2 % (V/V).…”

Section: Methodsmentioning

confidence: 99%

Ability of indigenous Bacillus licheniformis and Bacillus subtilis in microbial enhanced oil recovery

Haghighat

Sepahy

Assadi

et al. 2008

Int. J. Environ. Sci. Technol.

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“…The 500 mL of salt solution was prepared by adding the various salts such as KH 2 PO 4 , 20 g; K 2 HPO 4 , 5.0 g, (NH 4 (Francy et al, 1991). One-half SNB medium with 2 % Bacto Agar (SNA) was used to isolate heterotrophs.…”

Section: Media and Cultivation Conditions Isolation Mediummentioning

confidence: 99%

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Zaki

Farag

Elreesh

et al. 2011

Int. J. Environ. Sci. Technol.

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ABSTRACT:The aim of this study was to look for high efficient bioflocculant-producing microorganisms. Among 36 bacterial colonies isolated from a crude petroleum oil sample, three of them as Bacillus subtilis and Pseudomonas spp. exhibited flocculation activity exceeding 90 % after 3 days of cultivation. They were identified by 16 S rDNA sequence analysis as Bacillus subtilis and Pseudomonas sp. Spectroscopic analysis of the polymers by nuclear magnetic resonance and fourier-transform infrared revealed that the polymers were glycoproteins. These polymers were soluble in water and insoluble in any organic solvents tested. The effects of bioflocculant dosage, temperature and pH on the flocculation activity were evaluated. The maximum bioflocculation activities were observed at an optimum bioflocculant dosage of 3.5 mg/L (strains Bacillus subtilis and Pseudomonas) and 5.0 mg/L (strain CPO14), respectively. In addition, these biopolymers were able to flocculate kaolin suspension (5 g/L) over a wide range of pH (pH 3-9) and temperature (5-50 °C) tested in the presence of CaCl 2 . The highest flocculation activities of strains CPO8, CPO13 and CPO14 were 96.03 %, 92.17 % and 97.59 %, respectively in the early stationary phase (at 24 h), while the cell production reached its maximum in the stationary phase (at 72 h). Their efficient flocculation capabilities suggest potential applications in industries.

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“…Embora a exata função fisiológica dos biossurfactantes ainda não tenha sido completamente elucidada, algumas funções têm sido atribuídas: -emulsificação e solubilização de hidrocarbonetos ou compostos insolúveis em água, facilitando o crescimento de microrganismos nestes substratos 9 . Porém, cepas de Bacillus subtilis produzem surfactantes apenas em substratos hidrossolúveis 10 ;…”

Section: Função Fisiológica Dos Biossurfactantesunclassified