Empowering Protein Engineering through Recombination of Beneficial Substitutions
Xinyue Wang,
Anni Li,
Xiujuan Li
et al.
Abstract:Directed evolution stands as a seminal technology for generating novel protein functionalities, a cornerstone in biocatalysis, metabolic engineering, and synthetic biology. Today, with the development of various mutagenesis methods and advanced analytical machines, the challenge of diversity generation and high‐throughput screening platforms is largely solved, and one of the remaining challenges is: how to empower the potential of single beneficial substitutions with recombination to achieve the epistatic effe… Show more
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