Employing nanoparticle tracking analysis of salivary neuronal exosomes for early detection of neurodegenerative diseases

Sharma, Vaibhav; Nikolajeff, Fredrik; Kumar, Saroj

doi:10.1186/s40035-023-00339-z

Cited by 15 publications

(7 citation statements)

References 132 publications

(137 reference statements)

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“…To facilitate early diagnosis of neurodegenerative illnesses, another study developed a screening approach that can be used in combination with other recognized subjective evaluation and imaging techniques. Based on the unique surface markers of neuron-derived exosomes, they can be quantified through fluorescence nanoparticle tracking analysis [ 93 ].…”

Section: Exosomes In the Diagnosis And Treatment Of Neuropsychiatric ...mentioning

confidence: 99%

Exosomes: potential targets for the diagnosis and treatment of neuropsychiatric disorders

Li,

Yuan,

Xie

et al. 2024

J Transl Med

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The field of neuropsychiatry is considered a middle ground between neurological and psychiatric disorders, thereby bridging the conventional boundaries between matter and mind, consciousness, and function. Neuropsychiatry aims to evaluate and treat cognitive, behavioral, and emotional disorders in individuals with neurological conditions. However, the pathophysiology of these disorders is not yet fully understood, and objective biological indicators for these conditions are currently lacking. Treatment options are also limited due to the blood–brain barrier, which results in poor treatment effects. Additionally, many drugs, particularly antipsychotic drugs, have adverse reactions, which make them difficult to tolerate for patients. As a result, patients often abandon treatment owing to these adverse reactions. Since the discovery of exosomes in 1983, they have been extensively studied in various diseases owing to their potential as nanocellulators for information exchange between cells. Because exosomes can freely travel between the center and periphery, brain-derived exosomes can reflect the state of the brain, which has considerable advantages in diagnosis and treatment. In addition, administration of engineered exosomes can improve therapeutic efficacy, allow lesion targeting, ensure drug stability, and prevent systemic adverse effects. Therefore, this article reviews the source and biological function of exosomes, relationship between exosomes and the blood–brain barrier, relationship between exosomes and the pathological mechanism of neuropsychiatric disorders, exosomes in the diagnosis and treatment of neuropsychiatric disorders, and application of engineered exosomes in neuropsychiatric disorders.

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Section: Exosomes In the Diagnosis And Treatment Of Neuropsychiatric ...mentioning

confidence: 99%

Exosomes: potential targets for the diagnosis and treatment of neuropsychiatric disorders

Li,

Yuan,

Xie

et al. 2024

J Transl Med

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“…In addition, they can be found in almost all human fluids, including saliva (sEV) [95]. In recent years, sEV has attracted increasing attention as a possible diagnostic tool for multiple diseases, including cancers and neurodegenerative disorders [95][96][97].…”

Section: Salivary Extracellular Vesicles (Sev)mentioning

confidence: 99%

Salivary Biomarkers for Parkinson’s Disease: A Systematic Review with Meta-Analysis

Nijakowski,

Owecki,

Jankowski

et al. 2024

Cells

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Parkinson’s Disease (PD) is a common neurodegenerative disease which manifests with motor features, such as bradykinesia, resting tremor, rigidity, and postural instability. Using the non-invasive technique of saliva collection, we designed a systematic review to answer the question “Are salivary biomarkers reliable for the diagnosis of Parkinson’s Disease?”. Following inclusion and exclusion criteria, 30 studies were included in this systematic review (according to the PRISMA statement guidelines). Mostly proteins were reported as potential biomarkers in saliva. Based on meta-analysis, in PD patients, salivary levels of total alpha-synuclein were significantly decreased, and those of oligomeric alpha-synuclein were significantly increased. Also, according to pooled AUC, heme oxygenase-1 demonstrated significant predictive value for saliva-based PD diagnosis. In conclusion, some potential biomarkers, especially alpha-synuclein, can be altered in the saliva of PD patients, which could be reliably useful for early diagnosis of this neurodegenerative disease differentiating other synucleopathies.

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“…Among them, dynamic light scattering (DLS) ,− is the most widely used method, which records the scattered light fluctuations caused by the Brownian motion of particles. Derivatively, fluorescence correlation spectroscopy − measures fluorescence fluctuations to evaluate the HD of fluorescently labeled molecules, and nanoparticle tracking analysis − solves HD by tracking and locating scattered light images of minority/single particles to calculate particle diffusion velocity. However, Brownian motion is a random motion with a characteristic time of a few submilliseconds at the nanoscale .…”

Section: Introductionmentioning

confidence: 99%

Dynamic Monitoring of Biomolecular Hydrodynamic Dimensions by Magnetization Motion on Quartz Crystal Microbalance

Zuo,

Wen,

Chen

et al. 2024

Anal. Chem.

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Hydrodynamic dimension (HD) is the primary indicator of the size of bioconjugated particles and biomolecules. It is an important parameter in the study of solid−liquid two-phase dynamics. HD dynamic monitoring is crucial for precise and customized medical research as it enables the investigation of the continuous changes in the physicochemical characteristics of biomolecules in response to external stimuli. However, current HD measurements based on Brownian motion, such as dynamic light scattering (DLS), are inadequate for meeting the polydisperse sample demands of dynamic monitoring. In this paper, we propose MMQCM method samples of various types and HD dynamic monitoring. An alternating magnetic field of frequency ω m excites biomolecule-magnetic bead particles (bioMBs) to generate magnetization motion, and the quartz crystal microbalance (QCM) senses this motion to provide HD dynamic monitoring. Specifically, the magnetization motion is modulated onto the thickness-shear oscillation of the QCM at the frequency ω q . By analysis of the frequency spectrum of the QCM output signal, the ratio of the magnitudes of the real and imaginary parts of the components at frequency ω q ± 2ω m is extracted to characterize the particle size. Using the MMQCM approach, we successfully evaluated the size of bioMBs with different biomolecule concentrations. The 30 min HD dynamic monitoring was implemented. An increase of ∼10 nm in size was observed upon biomolecular structural stretching. Subsequently, the size of bioMBs gradually reduced due to the continuous dissociation of biomolecules, with a total reduction of 20∼40 nm. This HD dynamic monitoring demonstrates that the release of biomolecules can be regulated by controlling the duration of magnetic stimulation, providing valuable insights and guidance for controlled drug release in personalized precision medicine.

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Employing nanoparticle tracking analysis of salivary neuronal exosomes for early detection of neurodegenerative diseases

Cited by 15 publications

References 132 publications

Exosomes: potential targets for the diagnosis and treatment of neuropsychiatric disorders

Exosomes: potential targets for the diagnosis and treatment of neuropsychiatric disorders

Salivary Biomarkers for Parkinson’s Disease: A Systematic Review with Meta-Analysis

Dynamic Monitoring of Biomolecular Hydrodynamic Dimensions by Magnetization Motion on Quartz Crystal Microbalance

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