Employing human keratinocytes cultured on macroporous gelatin spheres to treat full thickness-wounds: An in vivo study on athymic rats

Gustafson, Carl-Johan; Birgisson, Agust; Junker, Johan P.E.; Huss, Fredrik; Salemark, Lars; Johnson, Hans; Kratz, Gunnar

doi:10.1016/j.burns.2006.10.382

Cited by 24 publications

(11 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gelatin is a degradation product of collagen and is further degraded without any toxic waste products. Earlier studies within the research group have shown that the porous gelatin spheres are a suitable matrix for guided tissue regeneration [114,115]. They can be used as a liquid and injectable three-dimensional scaffold.…”

Section: Main Results and Discussionmentioning

confidence: 99%

Guided Regeneration of the Human Skin : in vitro and in vivo studies

Nyman¹

2015

Linköping University Medical Dissertations

View full text Add to dashboard Cite

show abstract

Section: Main Results and Discussionmentioning

confidence: 99%

Guided Regeneration of the Human Skin : in vitro and in vivo studies

Nyman¹

2015

Linköping University Medical Dissertations

View full text Add to dashboard Cite

show abstract

“…This technique has been used to produce microsphere carriers at a large scale. The average internal pore size of the microspheres generated is between 10–20 µm . Microcarriers with pore size higher than 50 µm support growth and enhance matrix deposition by chondrocytes, than those below 50 µm .…”

Section: Technologies For Fabrication Of Microscale Cell‐delivery Plamentioning

confidence: 99%

Toward Customized Extracellular Niche Engineering: Progress in Cell‐Entrapment Technologies

2017

View full text Add to dashboard Cite

The primary aim in tissue engineering is to repair, replace, and regenerate dysfunctional tissues to restore homeostasis. Cell delivery for repair and regeneration is gaining impetus with our understanding of constructing tissue‐like environments. However, the perpetual challenge is to identify innovative materials or re‐engineer natural materials to model cell‐specific tissue‐like 3D modules, which can seamlessly integrate and restore functions of the target organ. To devise an optimal functional microenvironment, it is essential to define how simple is complex enough to trigger tissue regeneration or restore cellular function. Here, the purposeful transition of cell immobilization from a cytoprotection point of view to that of a cell‐instructive approach is examined, with advances in the understanding of cell–material interactions in a 3D context, and with a view to further application of the knowledge for the development of newer and complex hierarchical tissue assemblies for better examination of cell behavior and offering customized cell‐based therapies for tissue engineering.

show abstract

“…The chronic venous leg ulcers were thereby treated with this kind of keratinocyte culture and delivery method and had a higher healing rate and no recurrence at a 1-year follow-up (139,140,144). This technique could theoretically acquire more keratinocytes in a short period (145), and circumvent the dilemma in making a choice between the mechanical strength and the porosity of the dermal equivalent (138,140). However, more clinical trials are still needed to authenticate the effectiveness and efficacy on wound healing.…”

Section: Source and Scaling-up Considerations For Epidermal Cellsmentioning

confidence: 95%

“…This bioreactor system provides the continuous perfusion to supply nutrients to the cultured skin equivalent (135). The spinner flask in combination with the biodegradable microcarrier culture technique had also been used successfully in the cultivation of keratinocytes, which brought a new vision both in keratinocyte culture and the cell delivery technique (138)(139)(140)(141)(142)(143)(144). The porous gelatin microbeads (CultiSpher-G) are used in this culture system and as a direct delivery technique to the wound bed (145).…”

Section: Source and Scaling-up Considerations For Epidermal Cellsmentioning

confidence: 99%

Epidermal cells delivered for cutaneous wound healing

Wang

Sun

Wang

et al. 2010

Journal of Dermatological Treatment

View full text Add to dashboard Cite

Re-epithelialization is the first and most important step in cutaneous wound healing. The vital role of epidermal cells, or keratinocytes, in accelerating wound healing has long been established. The technique of delivering the cultured and uncultured epidermal cells to the wound bed takes a variety of forms including cultured epithelial autografts (CEAs), tissue-engineered skin equivalent, epidermal suspension and microbead-loaded composite. These techniques, together with the keratinocyte culturing method and scaling up equipment, are still the ongoing research. Application of these techniques also bears direct impact on the outcome of the wounded patients. Best understanding of the delivery technique and its relationship with the culturing method and delivery vehicle could benefit not only the wounded patient but also the development of tissue-engineered skin equivalent.

show abstract

Employing human keratinocytes cultured on macroporous gelatin spheres to treat full thickness-wounds: An in vivo study on athymic rats

Cited by 24 publications

References 28 publications

Guided Regeneration of the Human Skin : in vitro and in vivo studies

Guided Regeneration of the Human Skin : in vitro and in vivo studies

Toward Customized Extracellular Niche Engineering: Progress in Cell‐Entrapment Technologies

Epidermal cells delivered for cutaneous wound healing

Contact Info

Product

Resources

About