Emodin and rhapontigenin inhibit the replication of African swine fever virus by interfering with virus entry

Guo, Yanchen; Yang, Chen; Wang, Qiumei; Wang, Zhiyuan; Gong, Lang; Sun, Yankuo; Song, Zebu; Chang, Hao; Zhang, Guihong; Wang, Heng

doi:10.1016/j.vetmic.2023.109794

Cited by 3 publications

(2 citation statements)

References 54 publications

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“…Among them, emodin, aloe emodin, and thea avin have the best inhibitory effect and highest safety. Emodin and aloe emodin exhibit the ability to inhibit more than ten viral infections in vitro and in vivo, including herpes simplex virus (HSV-1) [23,24], African swine fever (ASFV) [25], human cytomegalovirus (HCMV) [26], coxsackie virus B (CVB) [27,28], enterovirus 71 (EV71) [29,30] and in uenza A virus [31,32], with spectral antiviral properties. Our study found that emodin and aloe emodin can inhibit the replication of LSDV, with IC 50 of 10.86 µM and 10.13 µ M, respectively.…”

Section: Discussionmentioning

confidence: 99%

Construction of recombinant LSDV fluorescent virus for high-throughput screening of antiviral drugs

Wang,

Ji,

zhong

et al. 2023

Preprint

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Lumpy skin disease virus (LSDV) infection is a major socio-economic issue that can cause serious threat to the global cattle farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP of expressing EGFP was constructed using a homologous recombination system and applied to high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP can replicate in various kidney cell lines to consistent with wild-type LSDV. At the same time, the cytopathic effects, virus particle morphology, and growth performance caused by LSDV-ΔTK/EGFP are consistent with those of wild LSDV. High-throughput screening found that emodin (for LSDV-ΔTK/EGFP, IC50 = 10.86 µM), aloe emodin (for LSDV-ΔTK/EGFP, IC50 = 10.13 µM), theaflavin (for LSDV-ΔTK/EGFP, IC50 = 10.05 µM), 4-ethylphenol (for LSDV-ΔTK/EGFP, IC50 = 32.17 µM), tulipalin (for LSDV-ΔTK/EGFP, IC50 = 28.58 µM) and anemoside B4 (for LSDV-ΔTK/EGFP, IC50 = 845.65 µM) inhibited LSDV replication. Theaflavin strong inhibitory effect on LSDV was identified from 100 antiviral drugs in vitro. In addition, the addition time measurement shown that theaflavins play a role in the entry of LSDV into cells and subsequent viral replication stages. The development of this recombinant virus contributes to the development of LSDV antiviral drugs and the subsequent study of virus replication and mechanism of action.

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Section: Discussionmentioning

confidence: 99%

Construction of recombinant LSDV fluorescent virus for high-throughput screening of antiviral drugs

Wang,

Ji,

zhong

et al. 2023

Preprint

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“…Emodin is prevalent in more than 800 types of Chinese medicinal formulations [ 1 ](as shown in Table 1 ). A multitude of prior investigations have ascertained that emodin exhibits a diverse array of pharmacological effects, encompassing its roles as an anticancer agent [ 2 ], an anti-inflammatory agent [ 3 ], an antiviral agent [ 4 ], and an antioxidant [ 5 ]. These properties have rendered emodin a valuable ingredient in Chinese medicinal preparations and dietary supplements.…”

Section: Introductionmentioning

confidence: 99%

Advances in the mechanism of emodin-induced hepatotoxicity

Wang,

Zhao,

et al. 2024

Heliyon

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Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs

Wang,

Ji,

Zhong

et al. 2024

Vet Res

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Lumpy skin disease virus (LSDV) infection is a major socio-economic issue that seriously threatens the global cattle-farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP, expressing enhanced green fluorescent protein (EGFP), was constructed with a homologous recombination system and applied to the high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP replicates in various kidney cell lines, consistent with wild-type LSDV. The cytopathic effect, viral particle morphology, and growth performance of LSDV-ΔTK/EGFP are consistent with those of wild-type LSDV. High-throughput screening allowed to identify several molecules that inhibit LSDV-ΔTK/EGFP replication. The strong inhibitory effect of theaflavin on LSDV was identified when 100 antiviral drugs were screened in vitro. An infection time analysis showed that theaflavin plays a role in the entry of LSDV into cells and in subsequent viral replication stages. The development of this recombinant virus will contribute to the development of LSDV-directed antiviral drugs and the study of viral replication and mechanisms of action.

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Emodin and rhapontigenin inhibit the replication of African swine fever virus by interfering with virus entry

Cited by 3 publications

References 54 publications

Construction of recombinant LSDV fluorescent virus for high-throughput screening of antiviral drugs

Construction of recombinant LSDV fluorescent virus for high-throughput screening of antiviral drugs

Advances in the mechanism of emodin-induced hepatotoxicity

Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs

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