Emission features of microstructures fabricated by two-photon polymerization containing three organic dyes

Incorporation of a FRET dye pair into mesoporous materials: a comparison of fluorescence spectra, FRET activity and dye accessibility

“…Gaussian fitting to photoluminescence spectra is a common procedure for identifying the involved species which contribute to the final entire emission band. [55][56][57] To evaluate the contribution of the increasing fluorescein emission upon NH 3 exposure, to the increasing rhodamine emission, Gaussian fitting for the PG SBA FR 1 emission bands was performed. Only fluorescein and rhodamine labeled particles were fitted to find the peak wavelengths and fluorescence tail lengths of each species needed to account for the final spectra.…”

Direct laser writing by two-photon polymerization as a tool for developing microenvironments for evaluation of bacterial growth

“…Again, upon finishing the microstructure fabrication, the sample was rinsed in ethanol to remove unpolymerized resin. This approach does not limit the shape or number of microstructures which may be produced [17]. In general, the dopant content (antibiotic in this case) retained into the bulk of two-photon polymerized microstructures is dependent on their dimensions.…”

“…Such approach enables the fabrication of devices with specific characteristics for different areas of optics [8][9][10] and biology [11][12][13][14][15][16]. Furthermore, the manufacture of components using 2PP can be carried out in stages, allowing the fabrication of microdevices with multi-doping components [17]. The application of 2PP has recently been used for fabricating microenvironments and biological devices [18][19][20][21], allowing major advances in drug delivery systems and studies of the process of three-dimensional cell migration [15,[22][23][24][25].…”

Femtosecond lasers for processing glassy and polymeric materials

“…Two-photon absorption polymerization (2PP) has emerged as a promising technique for fabricating polymeric microdevices due to the advantages it provides 16,17 ; (i) resolution below the diffraction limit because of the quadratic dependence on the intensity, (ii) no restrictions regarding the shape the 3D fabricated structures and (iii) the capability of producing components with moving parts. In the last few years, the 2PP fabrication of microstructures containing compounds of interest, such as fluorescent dyes and nanoparticles, has been demonstrated in polymeric structures [18][19][20][21] , as well as in hybrid organic-inorganic materials 22 , with interesting prospects for the development of devices 14, 23 . These structures can be specially designed to meet the desired application 24 .…”