Emerging Themes in Lipid Rafts and Caveolae

Galbiati, Ferruccio; Razani, Babak; Lisanti, Michael P.

doi:10.1016/s0092-8674(01)00472-x

Cited by 551 publications

(504 citation statements)

References 48 publications

Supporting

Mentioning

496

Contrasting

Order By: Relevance

“…SFKs are thought to be localized predominantly at lipid rafts (29,44), and we found that the ganglioside GM1, a marker for lipid rafts (29,30), was also enriched and localized along the apical surface of crypt IECs. Lipid rafts may therefore recruit SFKs to the apical surface of crypt IECs.…”

Section: Discussionmentioning

confidence: 65%

See 1 more Smart Citation

Role of Src Family Kinases in Regulation of Intestinal Epithelial Homeostasis

Imada

Murata

Kotani

et al. 2016

Molecular and Cellular Biology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 65%

“…S6A). Moreover, staining of the ganglioside GM1 as a marker for lipid rafts (29,30) with the B subunit of cholera toxin revealed a pattern similar to that apparent for the three SFKs in intestinal crypts (see Fig. S6B).…”

Section: Generation Of Iec-specific Csk Cko Micementioning

confidence: 59%

Role of Src Family Kinases in Regulation of Intestinal Epithelial Homeostasis

Imada

Murata

Kotani

et al. 2016

Molecular and Cellular Biology

View full text Add to dashboard Cite

“…For endothelia of several organs of the body, experimental evidence points to the existence of what has been referred to as two sets of physiological pores: large pores responsible for the transport of proteins and small pores designed for the diffusion of small solutes. Opinions differ widely concerning which features of endothelial cells found in these organsfenestrae, the transtubulovesicular system including caveolae, and interendothelial clefts-are the morphological correlates to the two pore system suggested by the physiological data (Predescu et al, 1997;Michel and Curry, 1999;Silverstein, 1999;Smart et al, 1999;Galbiati et al, 2001;Bendayan, 2002;Feng et al, 2002;Simionescu et al, 2002).…”

Section: Biomolecular Size Exclusion Of Dextrans Equal To or Greater mentioning

confidence: 99%

In vivo delivery of fluoresceinated dextrans to the murine growth plate: Imaging of three vascular routes by multiphoton microscopy

et al. 2005

View full text Add to dashboard Cite

Bone elongation by endochondral ossification occurs through the differentiation cascade of chondrocytes of cartilaginous growth plates. Molecules from the systemic vasculature reach the growth plate from three different directions: epiphyseal, metaphyseal, and a ring vessel and plexus associated with the perichondrium. This study is an analysis of the real-time dynamics of entrance of fluoresceinated tracers of different molecular weights into the growth plate from the systemic vasculature and tests the hypothesis that molecular weight is a key variable in the determination of both the directionality and the extent of tracer movement into the growth plate. Multiphoton microscopy was used for direct in vivo imaging of the murine proximal tibial growth plate in anesthetized 4-to 5-week-old transgenic mice with green fluorescent protein linked to the collagen II promoter. Mice were given an intracardiac injection of either fluorescein (332.3 Da) or fluoresceinated dextrans of 3, 10, 40, 70 kDa, singly or sequentially. For each tracer, directionality and rate of arrival, together with extent of movement within the growth plate, were imaged in real time. For small molecules (up to 10 kDa), vascular access from all three directions was observed and entrance was equally permissive from the metaphyseal and the epiphyseal sides. Within our detection limit (a few percent of vascular concentration), 40 kDa and larger dextrans did not enter. These results have implications both for understanding systemic and paracrine regulation of growth plate chondrocytic differentiation, as well as variables associated with effective drug delivery to growth plate chondrocytes. Key words: growth plate; vasculature; multiphoton microscopy; endochondral ossificationBone elongation in children occurs by endochondral ossification in cartilaginous growth plates at the ends of long bones. Chondrocytic differentiation in the growth plate begins with clonal expansion of stem cells, continues during cellular proliferation, and ends with cellular enlargement, followed by death and replacement by bone. The kinetics of chondrocytic activity in each stage and of the regulatory transitions between them determine the rate of bone elongation achieved. As in any cellular growth system, nutrients are required to sustain the process, and multiple endocrine and paracrine inputs regulate at each stage. For growth plate cartilage, the role of the extracellular matrix also must be considered, not only as a substantive contributor to elongation per se, but through its potential role as a communication link among chondrocytes of the growth plate, and between growth plate chondrocytes and cells of surrounding tissues such as the peri-

show abstract

“…Therefore, specific membrane compartments are required in order to concentrate and organize these signal molecules. These specific membrane compartments, also commonly referred to as the lipid rafts, are composed of cholesterol and glycolipids [1,2], and predominantly harbor a host of receptors and signaling molecules, allowing them to mediate a variety of cellular events, including cellular signaling, pathogenic invasion, immune responses, and cholesterol homeostasis [3][4][5][6]. Because the lipid rafts are loaded with many receptors for epidermal growth factor (EGF) [7], platelet-derived growth factor [8], nerve growth factor [9], insulin [10], insulin-like growth factor [11], transforming growth factor [12], and tumor necrosis factor [13], these rafts might act as platforms which initiate various cellular signal pathways.…”

Section: Introductionmentioning

confidence: 99%

Oxidation–reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts

Ki-Bum

Lee

et al. 2006

Proteomics

View full text Add to dashboard Cite

In order to detect and identify ubiquitous lipid raft marker proteins, we isolated lipid rafts from different mouse organs, including the liver, lung, large brain, and kidney, and analyzed their proteins via 2-DE. Many protein spots were determined to be ubiquitous in all of the lipid rafts, and were annotated via LC and MS/MS. Twelve proteins were identified as ubiquitous raft proteins, and most of these were determined to be mitochondrial proteins, including mortalin, prohibitin, voltage-dependent anion channel, ATP synthase, NADH dehydrogenase, and ubiquinol-cytochrome c reductase. Via immunoblotting, these proteins were shown to exist in detergent-resistant lipid rafts prepared using different organ tissues. Since these oxidationreduction respiratory chains and ATP synthase complex were detected in detergent-resistant lipid raft fractions which had been isolated from the plasma membrane but not from the mitochondria, and found in the cell surface when determined by immunofluoresence and immunohistochemistry, we conclude that plasma membrane lipid rafts might contain oxidation-reduction respiratory chains and ATP synthase complex.

show abstract

Emerging Themes in Lipid Rafts and Caveolae

Cited by 551 publications

References 48 publications

Role of Src Family Kinases in Regulation of Intestinal Epithelial Homeostasis

Role of Src Family Kinases in Regulation of Intestinal Epithelial Homeostasis

In vivo delivery of fluoresceinated dextrans to the murine growth plate: Imaging of three vascular routes by multiphoton microscopy

Oxidation–reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts

Contact Info

Product

Resources

About