Emerging strategies to enhance the sensitivity of competitive ELISA for detection of chemical contaminants in food samples

Xiong, Ying; Leng, Yuankui; Li, Xiangmin; Huang, Xiaolin; Xiong, Yonghua

doi:10.1016/j.trac.2020.115861

Cited by 121 publications

(44 citation statements)

References 147 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the extensive use of antibiotics in animal feed in order to increase production is associated with the spread of resistant bacterial strains for which current treatments are ineffective [ 1 ]. Despite their high sensitivity, many of the traditional assays employed for detection of food contaminants, such as HPLC and mass spectrometry, are expensive, laborious, and demand highly trained staff, and therefore have limited suitability for areas with poorly equipped facilities and shortages of specialists [ 7 , 8 , 9 , 10 , 11 , 12 ]. In this context, the efforts to develop accurate, easy-to-use, disposable, and inexpensive devices for food safety control have greatly expanded.…”

Section: Introductionmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

View full text Add to dashboard Cite

Food safety monitoring assays based on synthetic recognition structures such as aptamers are receiving considerable attention due to their remarkable advantages in terms of their ability to bind to a wide range of target analytes, strong binding affinity, facile manufacturing, and cost-effectiveness. Although aptasensors for food monitoring are still in the development stage, the use of an electrochemical detection route, combined with the wide range of materials available as transducers and the proper immobilization strategy of the aptamer at the transducer surface, can lead to powerful analytical tools. In such a context, employing aryldiazonium salts for the surface derivatization of transducer electrodes serves as a simple, versatile and robust strategy to fine-tune the interface properties and to facilitate the convenient anchoring and stability of the aptamer. By summarizing the most important results disclosed in the last years, this article provides a comprehensive review that emphasizes the contribution of aryldiazonium chemistry in developing electrochemical aptasensors for food safety monitoring.

show abstract

Section: Introductionmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

View full text Add to dashboard Cite

show abstract

“…Among them, an ELISA is the most popular for the high-throughput screening of OTA-polluted samples because of its high inspection speed, low cost and excellent specificity [9,10]. However, a conventional ELISA suffers from limited sensitivity because of the low signal intensity of enzymatic reaction-derived chromogenic products [11,12]. Moreover, using enzyme-OTA conjugates as competing antigens leads to a serious secondary pollution and poses a noticeable occupational hazard [13] because of the consumption of toxic OTA and toxic organic reagents for its preparation.…”

Section: Introductionmentioning

confidence: 99%

Eco-Friendly Fluorescent ELISA Based on Bifunctional Phage for Ultrasensitive Detection of Ochratoxin A in Corn

Tong

Fang

Xiong

et al. 2021

Foods

Self Cite

View full text Add to dashboard Cite

Conventional enzyme-linked immunosorbent assay (ELISA) is commonly used for Ochratoxin A (OTA) screening, but it is limited by low sensitivity and harmful competing antigens of enzyme-OTA conjugates. Herein, a bifunctional M13 bacteriophage with OTA mimotopes fused on the p3 protein and biotin modified on major p8 proteins was introduced as an eco-friendly competing antigen and enzyme container for enhanced sensitivity. Mercaptopropionic acid-modified quantum dots (MPA-QDs), which are extremely sensitive to hydrogen peroxide, were chosen as fluorescent signal transducers that could manifest glucose oxidase-induced fluorescence quenching in the presence of glucose. On these bases, a highly sensitive and eco-friendly fluorescent immunoassay for OTA sensing was developed. Under optimized conditions, the proposed method demonstrates a good linear detection of OTA from 4.8 to 625 pg/mL and a limit of detection (LOD) of 5.39 pg/mL. The LOD is approximately 26-fold lower than that of a conventional horse radish peroxidase (HRP) based ELISA and six-fold lower than that of a GOx-OTA conjugate-based fluorescent ELISA. The proposed method also shows great specificity and accepted accuracy for analyzing OTA in real corn samples. The detection results are highly consistent with those obtained using the ultra-performance liquid chromatography-fluorescence detection method, indicating the high reliability of the proposed method for OTA detection. In conclusion, the proposed method is an excellent OTA screening platform over a conventional ELISA and can be easily extended for sensing other analytes by altering specific mimic peptide sequences in phages.

show abstract

“…By contrast, immunologic methods are widely used as screening tools because of their excellent specificity, simplicity, rapidity, and low cost. ELISA is the most popular screening platform because it has a high throughput and excellent specificity, and is robust and easy to automate [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Dramatically Enhancing the Sensitivity of Immunoassay for Ochratoxin A Detection by Cascade-Amplifying Enzyme Loading

Song

Feng

Leng

et al. 2021

Toxins

Self Cite

View full text Add to dashboard Cite

Enzyme-linked immunosorbent assay (ELISA) is widely used in the routine screening of mycotoxin contamination in various agricultural and food products. Herein, a cascade-amplifying system was introduced to dramatically promote the sensitivity of an immunoassay for ochratoxin A (OTA) detection. Specifically, a biotinylated M13 bacteriophage was introduced as a biofunctional competing antigen, in which a seven-peptide OTA mimotope fused on the p3 protein of M13 was used to specifically recognize an anti-OTA monoclonal antibody, and the biotin molecules modified on capsid p8 proteins were used in loading numerous streptavidin-labeled polymeric horseradish peroxidases (HRPs). Owing to the abundance of biotinylated p8 proteins in M13 and the high molar ratio between HRP and streptavidin in streptavidin-polyHRP, the loading amount of HRP enzymes on the M13 bacteriophage were greatly boosted. Hence, the proposed method exhibited high sensitivity, with a limit of detection of 2.0 pg/mL for OTA detection, which was 250-fold lower than that of conventional ELISA. In addition, the proposed method showed a slight cross-reaction of 2.3% to OTB, a negligible cross-reaction for other common mycotoxins, and an acceptable accuracy for OTA quantitative detection in real corn samples. The practicability of the method was further confirmed with a traditional HRP-based ELISA method. In conclusion, the biotinylated bacteriophage and polyHRP structure showed potential as a cascade-amplifying enzyme loading system for ultra-trace OTA detemination, and its application can be extended to the detection of other analytes by altering specific mimic peptide sequences.

show abstract

Emerging strategies to enhance the sensitivity of competitive ELISA for detection of chemical contaminants in food samples

Cited by 121 publications

References 147 publications

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Eco-Friendly Fluorescent ELISA Based on Bifunctional Phage for Ultrasensitive Detection of Ochratoxin A in Corn

Dramatically Enhancing the Sensitivity of Immunoassay for Ochratoxin A Detection by Cascade-Amplifying Enzyme Loading

Contact Info

Product

Resources

About