Emerging strategies in 3D printed tissue models for in vitro biomedical research

Pless, Christian Jonathan; Radeke, Carmen; Cakal, Selgin D.; Kajtez, Janko; Pasqualini, Francesco S.; Lind, Johan

doi:10.1016/b978-0-323-85430-6.00007-8

Cited by 2 publications

(2 citation statements)

References 139 publications

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“…Beyond wearable sensors, the SEBS-based inks are of relevance for various other soft sensor applications, not least within laboratory models of mechanically active tissues, for example, in the form of instrumented micro-physiological systems and organs on chips. [2,3,22] In this regard, SEBS has been highlighted as a promising alternative elastomer to widely used PDMS, [22,23] which is hampered by unwanted side-effects such as adsorption of hydrophobic drugs. For such applications, the ability to print micrometer scale traces, as demonstrated here, is of particular relevance.…”

Section: Discussionmentioning

confidence: 99%

Soft Electronic Block Copolymer Elastomer Composites for Multi‐Material Printing of Stretchable Physiological Sensors on Textiles

Pless

Nikzad

Papiano

et al. 2023

Adv Elect Materials

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Soft and stretchable electronic materials have a number of unique applications, not least within sensors for monitoring human health. Through development of appropriate inks, micro‐extrusion 3D printing offers an appealing route for integrating soft electronic materials within wearable garments. Toward this objective, here a series of conductive inks based on soft thermoplastic styrene–ethylene–butylene–styrene elastomers combined with silver micro‐flakes, carbon black nanoparticles, or poly(3,4‐ethylenedioxythiophene) (PEDOT) conducting polymer additives, is developed. Their electrical and mechanical properties are systematically compared and found to be highly dependent on additive amount and type. Thus, while silver composites offer the highest conductivity, their stretchability is far inferior to carbon black composites, which can maintain conductivity beyond 400% strain. The PEDOT composites are the least conductive and stretchable but display unique properties due to their propensity for ionic conductivity. To integrate these inks, as well as insulating counterparts, into functional designs, a multi‐material micro‐extrusion 3D printing routine for direct deposition onto stretchable, elastic fabrics is established. As demonstration, prototypes are produced for sensing common health markers including strain, physiological temperatures, and electrocardiograms. Collectively, this work demonstrates multi‐material 3D printing of soft styrene–ethylene–butylene–styrene elastomer composites as a versatile method for fabricating soft bio‐sensors.

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Section: Discussionmentioning

confidence: 99%

Soft Electronic Block Copolymer Elastomer Composites for Multi‐Material Printing of Stretchable Physiological Sensors on Textiles

Pless

Nikzad

Papiano

et al. 2023

Adv Elect Materials

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“…Extrusion-based three-dimensional (3D) printing is redefining in vitro and in vivo biomedical research by enabling the automated fabrication of complex biomaterial scaffolds, engineered tissues, and microphysiological systems . Across these areas, a core challenge in 3D bioprinting is the formulation of biomaterial inks that facilitate the formation of functional tissues from embedded cells or spheroids, while simultaneously assuring printability and shape fidelity. , Bioinks incorporating nano- and microfibrils are intriguing in both of these regards. In the first regard, fibrillar inks may structurally mimic extracellular matrix (ECM) nanofibers derived from, e.g., collagen and fibronectin that guide cellular adhesion, migration, proliferation, differentiation, and organization in the native tissue .…”

Section: Introductionmentioning

confidence: 99%

Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks

Radeke

Pons

Mihajlovic

et al. 2023

ACS Appl. Mater. Interfaces

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For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissuemimicking constructs, there is a need for developing bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties. Toward this goal, we here present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities. Our core methodology relies on tailoring the size and dispersibility of cellulose fibrils through a solvent-controlled partial carboxymethylation. This way, we generate partially negatively charged cellulose nanofibers with diameters of ∼250 nm and lengths spanning tens to hundreds of microns. In this range, the fibers structurally match the size and dimensions of natural collagen fibers making them sufficiently large to orient cells. Yet, they are simultaneously sufficiently thin to be optically transparent. By adjusting fiber concentration, 3D printing inks with excellent shear-thinning properties can be established. In addition, as the fibers are readily dispersible, composite inks with both carbohydrates and extracellular matrix (ECM)derived proteins can easily be generated. We apply such composite inks for 3D printing cell-laden and cross-linkable structures, as well as tissue-guiding gel substrates. Interestingly, we find that the spatial organization of engineered tissues can be defined by the shear-induced alignment of fibers during the printing procedure. Specifically, we show how myotubes derived from human and murine skeletal myoblasts can be programmed into linear and complex nonlinear architectures on soft printed substrates with intermediate fiber contents. Our nanofibrillated cellulose inks can thus serve as a simple and scalable tool for engineering anisotropic human muscle tissues that mimic native structure and function.

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Emerging strategies in 3D printed tissue models for in vitro biomedical research

Cited by 2 publications

References 139 publications

Soft Electronic Block Copolymer Elastomer Composites for Multi‐Material Printing of Stretchable Physiological Sensors on Textiles

Soft Electronic Block Copolymer Elastomer Composites for Multi‐Material Printing of Stretchable Physiological Sensors on Textiles

Transparent and Cell-Guiding Cellulose Nanofiber 3D Printing Bioinks

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