Emerging Roles of TRIM Family Proteins in Gliomas Pathogenesis

Giannopoulou, Angeliki-Ioanna; Xanthopoulos, Charalampos; Piperi, Christina; Kostareli, Efterpi

doi:10.3390/cancers14184536

Cited by 4 publications

(3 citation statements)

References 131 publications

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“…Batch effect correction on raw count data for each transcriptome was performed using ComBat-seq [ 65 ]. The essential information regarding the TRIMs included in the analysis is detailed in Supplementary File S1 [ 66 , 67 , 68 ].…”

Section: Methodsmentioning

confidence: 99%

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Interleukin 27, Similar to Interferons, Modulates Gene Expression of Tripartite Motif (TRIM) Family Members and Interferes with Mayaro Virus Replication in Human Macrophages

Hernández-Sarmiento,

Tamayo-Molina,

Valdés-López

et al. 2024

Viruses

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Background: The Tripartite motif (TRIM) family includes more than 80 distinct human genes. Their function has been implicated in regulating important cellular processes, including intracellular signaling, transcription, autophagy, and innate immunity. During viral infections, macrophages are key components of innate immunity that produce interferons (IFNs) and IL27. We recently published that IL27 and IFNs induce transcriptional changes in various genes, including those involved in JAK-STAT signaling. Furthermore, IL27 and IFNs share proinflammatory and antiviral pathways in monocyte-derived macrophages (MDMs), resulting in both common and unique expression of inflammatory factors and IFN-stimulated genes (ISGs) encoding antiviral proteins. Interestingly, many TRIM proteins have been recognized as ISGs in recent years. Although it is already very well described that TRIM expression is induced by IFNs, it is not fully understood whether TRIM genes are induced in macrophages by IL27. Therefore, in this study, we examined the effect of stimulation with IL27 and type I, II, and III IFNs on the mRNA expression profiles of TRIM genes in MDMs. Methods: We used bulk RNA-seq to examine the TRIM expression profile of MDMs treated with IFNs or IL27. Initially, we characterized the expression patterns of different TRIM subfamilies using a heatmap. Subsequently, a volcano plot was employed to identify commonly differentially expressed TRIM genes. Additionally, we conducted gene ontology analysis with ClueGO to explore the biological processes of the regulated TRIMs, created a gene-gene interaction network using GeneMANIA, and examined protein-protein interactions with the STRING database. Finally, RNA-seq data was validated using RT-qPCR. Furthermore, the effect of IL27 on Mayaro virus replication was also evaluated. Results: We found that IL27, similar to IFNs, upregulates several TRIM genes’ expression in human macrophages. Specifically, we identified three common TRIM genes (TRIM19, 21, and 22) induced by IL27 and all types of human IFNs. Additionally, we performed the first report of transcriptional regulation of TRIM19, 21, 22, and 69 genes in response to IL27. The TRIMs involved a broad range of biological processes, including defense response to viruses, viral life cycle regulation, and negative regulation of viral processes. In addition, we observed a decrease in Mayaro virus replication in MDMs previously treated with IL27. Conclusions: Our results show that IL27, like IFNs, modulates the transcriptional expression of different TRIM-family members involved in the induction of innate immunity and an antiviral response. In addition, the functional analysis demonstrated that, like IFN, IL27 reduced Mayaro virus replication in MDMs. This implies that IL27 and IFNs share many similarities at a functional level. Moreover, identifying distinct TRIM groups and their differential expressions in response to IL27 provides new insights into the regulatory mechanisms underlying the antiviral response in human macrophages.

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Section: Methodsmentioning

confidence: 99%

“…Supplementary File S1. The gene symbol, subfamily symbol, and synonyms for each TRIM reported in the literature [ 66 , 67 , 68 ].…”

mentioning

confidence: 99%

Interleukin 27, Similar to Interferons, Modulates Gene Expression of Tripartite Motif (TRIM) Family Members and Interferes with Mayaro Virus Replication in Human Macrophages

Hernández-Sarmiento,

Tamayo-Molina,

Valdés-López

et al. 2024

Viruses

View full text Add to dashboard Cite

show abstract

“…A recent research has revealed that TRIM14 promotes 5-FU resistance in gastric cancer through regulating MAPK/mTOR pathway, suggesting possible role of TRIM14 protein in drug resistance through regulating signaling pathways [ 93 ]. In glioma, TRIM14 is upregulated, particularly in TMZ-resistance cells [ 94 ]. Deng et al revealed that upregulation of TRIM14 in glioma cells is related to circ_0005198/miR-198 axis [ 95 ].…”

Section: Relationship Between Circrna and Tmz Resistance In Glioma Tu...mentioning

confidence: 99%

The landscape of circRNAs in gliomas temozolomide resistance: Insights into molecular pathways

Mafi,

Hedayati,

Kahkesh

et al. 2024

Non-coding RNA Research

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TRIM6 promotes glioma malignant progression by enhancing FOXO3A ubiquitination and degradation

Guo,

Wang,

Zhang

et al. 2024

Translational Oncology

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Emerging Roles of TRIM Family Proteins in Gliomas Pathogenesis

Cited by 4 publications

References 131 publications

Interleukin 27, Similar to Interferons, Modulates Gene Expression of Tripartite Motif (TRIM) Family Members and Interferes with Mayaro Virus Replication in Human Macrophages

Interleukin 27, Similar to Interferons, Modulates Gene Expression of Tripartite Motif (TRIM) Family Members and Interferes with Mayaro Virus Replication in Human Macrophages

The landscape of circRNAs in gliomas temozolomide resistance: Insights into molecular pathways

TRIM6 promotes glioma malignant progression by enhancing FOXO3A ubiquitination and degradation

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