Emerging roles of protein kinase CK2 in abscisic acid signaling

Vilela, Belmiro; Pagès, Montserrat; Riera, Marta

doi:10.3389/fpls.2015.00966

Cited by 24 publications

(16 citation statements)

References 116 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2b), our collective data support the hypothesis that ABRE-binding transcription factors other than AREB/ABFs used in this study may activate ABRE-dependent gene expression downstream of CK2α1 in the absence of exogenous ABA. In contrast, considering the negative regulation model of CK2 in ABA signaling (Vilela et al 2015a, b), the results suggest that negative modulation of ABRE-dependent gene expression by CK2β may be involved in ABRE/SnRK2-mediated pathway through the negative regulation of SnRK2 in the absence of exogenous ABA.…”

Section: Discussionmentioning

confidence: 62%

Casein kinase 2 α and β subunits inversely modulate ABA signal output in Arabidopsis protoplasts

Nagatoshi

Fujita

2018

Planta

View full text Add to dashboard Cite

Main conclusionOur transient gene expression analyses in Arabidopsis protoplasts support the view that CK2αs and CK2βs positively and negatively modulate ABRE-dependent gene expression, respectively.The phytohormone abscisic acid (ABA) regulates the expression of thousands of genes via ABA-responsive elements (ABREs), and has a crucial role in abiotic stress response. Casein kinase II (CK2), a conserved Ser/Thr protein kinase in eukaryotes, is essential for plant viability. Although the CK2 has been known as a tetrameric holoenzyme comprised of two catalytic α and two regulatory β subunits, each of the two types of subunits has been proposed to have independent functions. The Arabidopsis genome encodes four α subunits (CK2α1, CK2α2, CK2α3, CK2α4) and four β subunits (CK2β1, CK2β2, CK2β3, CK2β4). There is a growing body of evidence linking CK2 to ABA signaling and abiotic stress responses. However, the roles of each CK2 subunit in ABA signaling remain largely elusive. Using the transient expression system with the core ABA signaling components in Arabidopsis leaf mesophyll protoplasts, we show here that CK2α1 and CK2α2 (CK2α1/2) positively modulate ABRE-dependent gene expression as ABA signal output in ABA signaling, whereas all four CK2βs negatively modulate the ABRE-dependent gene expression mediated by subclass III SnRK2–AREB/ABF pathway and by CK2α1/2. These data indicate that CK2α1/2 and CK2βs positively and negatively modulate ABA signal output, respectively, suggesting that the quantitative balance of CK2 subunits determines the ABA signal output in plants. Given that CK2s act as pleiotropic enzymes involved in multiple developmental and stress–responsive processes, our findings suggest that CK2 subunits may be involved in integration and coordination of ABA-dependent and -independent signaling.Electronic supplementary materialThe online version of this article (10.1007/s00425-018-2919-5) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 62%

Casein kinase 2 α and β subunits inversely modulate ABA signal output in Arabidopsis protoplasts

Nagatoshi

Fujita

2018

Planta

View full text Add to dashboard Cite

show abstract

“…However, it is assumed that the interplay of protein kinases, protein phosphatases and phytohormones participates in the signaling and metabolic networks that control the phosphorylation/dephosphorylation levels of storage proteins. The CK2 protein is a Ser/Thr kinase presents in all eukaryotes and has pleiotropic effects; it is also involved in the regulation of multiple plant growth and development processes and ABA signalling [ 136 , 137 , 138 ]. Irar et al [ 126 ] used 2-DE-based maps for the phosphoproteome profiling of heat-stable proteins from Arabidopsis dry seeds and phosphoaffinity chromatography for phosphoprotein enrichment.…”

Section: Advances In the Biology Of Storage Proteinsmentioning

confidence: 99%

Advances in the Biology of Seed and Vegetative Storage Proteins Based on Two-Dimensional Electrophoresis Coupled to Mass Spectrometry

et al. 2018

View full text Add to dashboard Cite

Seed storage proteins play a fundamental role in plant reproduction and human nutrition. They accumulate during seed development as reserve material for germination and seedling growth and are a major source of dietary protein for human consumption. Storage proteins encompass multiple isoforms encoded by multi-gene families that undergo abundant glycosylations and phosphorylations. Two-dimensional electrophoresis (2-DE) is a proteomic tool especially suitable for the characterization of storage proteins because of their peculiar characteristics. In particular, storage proteins are soluble multimeric proteins highly represented in the seed proteome that contain polypeptides of molecular mass between 10 and 130 kDa. In addition, high-resolution profiles can be achieved by applying targeted 2-DE protocols. 2-DE coupled with mass spectrometry (MS) has traditionally been the methodology of choice in numerous studies on the biology of storage proteins in a wide diversity of plants. 2-DE-based reference maps have decisively contributed to the current state of our knowledge about storage proteins in multiple key aspects, including identification of isoforms and quantification of their relative abundance, identification of phosphorylated isoforms and assessment of their phosphorylation status, and dynamic changes of isoforms during seed development and germination both qualitatively and quantitatively. These advances have translated into relevant information about meaningful traits in seed breeding such as protein quality, longevity, gluten and allergen content, stress response and antifungal, antibacterial, and insect susceptibility. This review addresses progress on the biology of storage proteins and application areas in seed breeding using 2-DE-based maps.

show abstract

“…In mammals, about 300 substrates have been identified. In contrast, as yet only 30 substrates have been confirmed for plant CK2 (Vilela et al, 2015). Nonetheless, plant CK2 is active in diverse pathways related to the cell cycle, DNA-damage response and repair (Moreno-Romero et al, 2012), biotic and abiotic stress response, hormonal response, phosphate homeostasis (Chen et al, 2015), lightsensitivity and the circadian clock (Marta et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Crystal structure of Arabidopsis thaliana casein kinase 2 α1

2020

View full text Add to dashboard Cite

Casein kinase 2 (CK2) is a ubiquitous pleiotropic enzyme that is highly conserved across eukaryotic kingdoms. CK2 is singular amongst kinases as it is highly rigid and constitutively active. Arabidopsis thaliana is widely used as a model system in molecular plant research; the biological functions of A. thaliana CK2 are well studied in vivo and many of its substrates have been identified.Here, crystal structures of the subunit of A. thaliana CK2 in three crystal forms and of its complex with the nonhydrolyzable ATP analog AMppNHp are presented. While the C-lobe of the enzyme is highly rigid, structural plasticity is observed for the N-lobe. Small but significant displacements within the active cleft are necessary in order to avoid steric clashes with the AMppNHp molecule. Binding of AMppNHp is influenced by a rigid-body motion of the N-lobe that was not previously recognized in maize CK2.

show abstract

Emerging roles of protein kinase CK2 in abscisic acid signaling

Cited by 24 publications

References 116 publications

Casein kinase 2 α and β subunits inversely modulate ABA signal output in Arabidopsis protoplasts

Casein kinase 2 α and β subunits inversely modulate ABA signal output in Arabidopsis protoplasts

Advances in the Biology of Seed and Vegetative Storage Proteins Based on Two-Dimensional Electrophoresis Coupled to Mass Spectrometry

Crystal structure of Arabidopsis thaliana casein kinase 2 α1

Contact Info

Product

Resources

About