Emerging roles of NAC transcription factor in medicinal plants: progress and prospects

Kumar, Ramesh; Das, S. K.; Mishra, Madhvi; Choudhury, Debjani; Sharma, Komal; Kumari, Abha; Singh, Rakesh

doi:10.1007/s13205-021-02970-x

Cited by 19 publications

(13 citation statements)

References 95 publications

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“…TFs function as important switches of transcriptional networks that accurately regulate gene expression ( Jiang et al, 2016 ; Droge-Laser et al, 2018 ; Kumar et al, 2021 ). NAC TF family has been identified as one of the largest plant-specific regulators involved in diverse biological processes of numerous plant species, such as Arabidopsis , rice, tomato, and maize ( Christiansen and Gregersen, 2014 ; Su et al, 2015 ; Singh et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization and Comprehensive Analysis of NAC Transcription Factor Family in Nelumbo nucifera

Song

Liu

Gao³

et al. 2022

Front. Genet.

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NAC (NAM, ATAF, and CUC) is a ubiquitously expressed plant-specific transcription factor (TF) family which is involved in the regulation of various biological processes. However, a systematic characterization of NAC gene family is yet to be reported in lotus. Here, 82 NnNAC genes which included five predicted membrane-bound NAC proteins were identified in the lotus genome. Phylogenetic analysis revealed seven-subfamily clusters (I–VII) of NnNAC proteins, with homologous gene pairs displaying similar conserved motifs and gene structure characteristics. Transactivation assay of NnNAC proteins revealed an extensive transcriptional activation capacity which is mediated by the highly divergent C-terminal activation domain (AD). Expression analysis of NnNAC genes in lotus tissues showed high transcript levels in root, stamen, petal and seed coat. In addition, 30 and 29 differentially expressed NnNAC candidate genes putatively involved in lotus seed development and response to complete submergence stress, respectively, were identified. Overall, our study provides potentially useful candidate gene resources for future molecular breeding of lotus varieties with novel agronomic traits.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization and Comprehensive Analysis of NAC Transcription Factor Family in Nelumbo nucifera

Song

Liu

Gao³

et al. 2022

Front. Genet.

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“…It has been reported that CNGCs play a key role in initiating the heat-stress response by regulating HSFs [ 70 , 71 ]. NAC TFs are a plant-specific family involved in multiple biological processes, including biotic and abiotic stress responses, growth, and development [ 72 ]. The association between CNGCs and NAC TFs has not been documented in the literature until now.…”

Section: Resultsmentioning

confidence: 99%

GhCNGC13 and 32 Act as Critical Links between Growth and Immunity in Cotton

Peng,

Li,

et al. 2023

IJMS

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Cyclic nucleotide-gated ion channels (CNGCs) remain poorly studied in crop plants, most of which are polyploid. In allotetraploid Upland cotton (Gossypium hirsutum), silencing GhCNGC13 and 32 impaired plant growth and shoot apical meristem (SAM) development, while triggering plant autoimmunity. Both growth hormones (indole-3-acetic acid and gibberellin) and stress hormones (abscisic acid, salicylic acid, and jasmonate) increased, while leaf photosynthesis decreased. The silenced plants exhibited an enhanced resistance to Botrytis cinerea; however, Verticillium wilt resistance was weakened, which was associated with LIPOXYGENASE2 (LOX2) downregulation. Transcriptomic analysis of silenced plants revealed 4835 differentially expressed genes (DEGs) with functional enrichment in immunity and photosynthesis. These DEGs included a set of transcription factors with significant over-representation in the HSF, NAC, and WRKY families. Moreover, numerous members of the GhCNGC family were identified among the DEGs, which may indicate a coordinated action. Collectively, our results suggested that GhCNGC13 and 32 functionally link to photosynthesis, plant growth, and plant immunity. We proposed that GhCNGC13 and 32 play a critical role in the “growth–defense tradeoff” widely observed in crops.

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“…Transcription factors are important modulators that could activate the biosynthesisassociated genes at the transcription level to improve the production of bioactive compounds [49,51]. Earlier reports suggest that putative WRKY, AP2/ERF, and bHLH transcription factors from A. paniculata were identified using genome or transcriptome data, and their real-time PCR-based expression studies were performed to understand their role in andrographolide biosynthesis [66,80,81].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, NAC transcription factors (AtNAC072, AtNAC55, and AtNAC019) are induced by ABA, high temperatures, and drought stress in A. thaliana [42]. Previous reports suggest that the NAC transcription factor modulates the expression of genes related to the secondary metabolite pathway, leading to the overproduction of secondary metabolites having medicinal values [49][50][51]. The coordinated regulation of secondary metabolite production via NAC transcription factors is an emerging field for the analysis of the net output, evoking signals, and controlling the signaling steps; examples include artemisinin and dihydroartemisinic acid content production in Artimisia annua [49], monoterpene production in Actinidia arguta [52], carotenoid production in Solanum lycopersicum [53], and caffeine production in Camellia sinensis [50].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, and Expression Analysis of NAC Transcription Factor from Andrographis paniculata (Burm. f.) Nees and Their Role in Andrographolide Production

Kumar,

Roy Choudhury

et al. 2024

Genes

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Andrographis paniculata (Burm. f.) Nees is an important medicinal plant known for its bioactive compound andrographolide. NAC transcription factors (NAM, ATAF1/2, and CUC2) play a crucial role in secondary metabolite production, stress responses, and plant development through hormonal signaling. In this study, a putative partial transcript of three NAC family genes (ApNAC83, ApNAC21 22 and ApNAC02) was used to isolate full length genes using RACE. Bioinformatics analyses such as protein structure prediction, cis-acting regulatory elements, and gene ontology analysis were performed. Based on in silico predictions, the diterpenoid profiling of the plant’s leaves (five-week-old) and the real-time PCR-based expression analysis of isolated NAC genes under abscisic acid (ABA) treatment were performed. Additionally, the expression analysis of isolated NAC genes under MeJA treatment and transient expression in Nicotiana tabacum was performed. Full-length sequences of three members of the NAC transcription factor family, ApNAC83 (1102 bp), ApNAC21 22 (996 bp), and ApNAC02 (1011 bp), were isolated and subjected to the promoter and gene ontology analysis, which indicated their role in transcriptional regulation, DNA binding, ABA-activated signaling, and stress management. It was observed that ABA treatment leads to a higher accumulation of andrographolide and 14-deoxyandrographolide content, along with the upregulation of ApNAC02 (9.6-fold) and the downregulation of ApNAC83 and ApNAC21 22 in the leaves. With methyl jasmonate treatment, ApNAC21 22 expression decreased, while ApNAC02 increased (1.9-fold), with no significant change being observed in ApNAC83. The transient expression of the isolated NAC genes in a heterologous system (Nicotiana benthamiana) demonstrated their functional transcriptional activity, leading to the upregulation of the NtHMGR gene, which is related to the terpene pathway in tobacco. The expression analysis and heterologous expression of ApNAC21 22 and ApNAC02 indicated their role in andrographolide biosynthesis.

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Emerging roles of NAC transcription factor in medicinal plants: progress and prospects

Cited by 19 publications

References 95 publications

Genome-Wide Characterization and Comprehensive Analysis of NAC Transcription Factor Family in Nelumbo nucifera

Genome-Wide Characterization and Comprehensive Analysis of NAC Transcription Factor Family in Nelumbo nucifera

GhCNGC13 and 32 Act as Critical Links between Growth and Immunity in Cotton

Isolation, Characterization, and Expression Analysis of NAC Transcription Factor from Andrographis paniculata (Burm. f.) Nees and Their Role in Andrographolide Production

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