Emerging roles of HOTAIR in human cancer

Background Long non-coding RNAs (LncRNAs) have recently been considered promising biomarkers for oncogenesis due to their epigenetic regulatory effects. HOTAIR is one of the oncogenic LncRNAs that was previously studied in different non-hematological malignancies. The current study set out to detect the expression level of HOTAIR LncRNA in AML patients concerning their clinical characteristics, laboratory data, FLT3-ITD, and NPM1 mutations, as well as treatment outcome. This study included quantitative detection of HOTAIR gene expression in 47 cases of AML using quantitative reverse transcription polymerase chain reaction, as well as NPM1 and FLT3-ITD genotyping. Results The HOTAIR expression was significantly higher in AML patients 6.87 (0.001) than in normal controls 1.66 (0.004–6.82) (p 0.007). The HOTAIR expression level was affected by chemotherapy, and it was correlated to hemoglobin level (p 0.001), age, total leukocytic count (p 0.022), and NPM1 mutation (p 0.017). HOTAIR gene expression level showed a correlation to relapse-free survival in the study group (p 0.04). Conclusion HOTAIR is overexpressed in patients with acute myeloid leukemia (AML). HOTAIR pre-treatment and post-chemotherapy gene expression levels can predict chemosensitivity and relapse.

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“…HOTAIR was reported to act as a bridge between protein complexes, supporting chromatin silencing, including the PRC2 [5].…”

Section: Discussionmentioning

confidence: 99%

“…HOX transcript antisense intergenic RNA (HOTAIR) is a 2158-bp LncRNA transcribed from the antisense strand of the homeobox C gene on chromosome 12 [4]. HOTAIR acts in trans on the HOX D locus of chromosome 2 through chromatin-remodeling enzymes [5]. It is a novel prognostic biomarker, especially for the potential metastatic prediction [6].…”

Section: Introductionmentioning

confidence: 99%

Study of HOTAIR LncRNA in AML patients in context to FLT3-ITD and NPM1 mutations status

Salah

Zawam

Fouad

et al. 2021

Egypt J Med Hum Genet

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“…Few studies have examined the effects of steroids, including P4, on lncRNA expression in cancer development. lncRNAs such as HOX transcript antisense intergenic RNA (HOTAIR) 35 and T‐cell leukemia/lymphoma 6 (TCL6) 36 were reported to be associated with the PR status of breast cancer, and may predict disease prognosis. lncRNAs such as metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) 37 and nuclear enriched abundant transcript 1 (NEAT1) 38 have been shown to regulate the cell cycle and influence the progression of endometrial cancer.…”

Section: Discussionmentioning

confidence: 99%

Microarray expression profiling of long noncoding RNAs in the progesterone‐treated lung cancer cells

Xie

Chen

2020

The Journal of Gene Medicine

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Background: The increasing incidence and unique biological features of lung cancer in women has prompted renewed interest in the role of sex hormones in this disease. We previously showed that progesterone (P4) inhibited lung cancer tumorigenesis and progression. Here, we investigated the effects of P4 on expression of long noncoding RNAs (lncRNAs) and target mRNAs in lung cancer cells. Methods: We performed high-throughput microarray and bioinformatics analysis to identify differentially expressed lncRNAs and mRNAs in the untreated and the P4-treated A549 human lung cancer cells. Results: In total, 692 lncRNAs and 268 mRNAs were significantly differentially expressed in the P4-treated A549 cells compared to the untreated A549 cells (> 2-fold change, p < 0.05). Of the lncRNAs, 82 and 610 were up-regulated and down-regulated, respectively. Gene ontology, pathway and network analyses showed that many of the mRNAs were involved in the regulation of classical pathways, including Notch signaling. Differential expression of a lncRNA signature composed of NONHSAT000264, FR075921, FR324124, linc-TRIM58, RP1-93H18.7, RP11-120 K9.2, RP11-134F2.2 and NONHSAG024980 was validated by quantitatuve reverse transcriptase-polymerase chain reaction analysis. Conclusions: This is the first report of differentially expressed lncRNAs in the P4-treated lung cancer cells. The results suggest that lncRNAs could serve as potential therapeutic targets for P4-sensitive lung cancer.

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“…HOTAIR (HOX antisense intergenic RNA); this lncRNA acts as a scaffold that recruits chromatin modifying complexes PRC2 (polycomb repressive complex 2) to the HOXD locus, coordinates H3K27 methylation and H3K4me2 demethylation of target genes, and contributes to maintaining a repressive chromatin state [ 27 ], which has been implicated in the pathogenesis of several kinds of cancers, including breast cancer, lung cancer, and colon cancer [ 28 ]. On the other hand, HOTTIP, which is transcribed from a locus upstream of HOXA genes, recruits the MLL-1/WDR5 complex to the 5’ region of HOXA genes, mediates H3K4me3 modification and activates transcription of the HOXA locus [ 29 ].…”

Section: Long Noncoding Rnas (Lncrnas)mentioning

confidence: 99%

Involvement of long noncoding RNAs in the pathogenesis of autoimmune diseases

Zou

2020

Journal of Translational Autoimmunity

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Autoimmune diseases are a group of heterogeneous disorders characterized by damage to various organs caused by abnormal innate and adaptive immune responses. The pathogenesis of autoimmune diseases is extremely complicated and has not yet been fully elucidated. Long noncoding RNAs (lncRNAs), which are defined as transcripts containing more than 200 nucleotides with no protein-coding capacity, are emerging as important regulators of gene expression via epigenetic modification, transcriptional regulation and posttranscriptional regulation. Accumulating evidence has demonstrated that lncRNAs play a key role in the regulation of immunological functions and autoimmunity. In this review, we discuss various molecular mechanisms by which lncRNAs regulate gene expression and recent findings regarding the involvement of lncRNAs in many human autoimmune diseases, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), idiopathic inflammatory myopathy (IIM), systemic sclerosis (SSc) and Sjögren’s syndrome (pSS).

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Emerging roles of HOTAIR in human cancer

Cited by 45 publications

References 150 publications

Study of HOTAIR LncRNA in AML patients in context to FLT3-ITD and NPM1 mutations status

Study of HOTAIR LncRNA in AML patients in context to FLT3-ITD and NPM1 mutations status

Microarray expression profiling of long noncoding RNAs in the progesterone‐treated lung cancer cells

Involvement of long noncoding RNAs in the pathogenesis of autoimmune diseases

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