Emerging Role of Metabolic Pathways in Abiotic Stress Tolerance

Jain, Mukesh

doi:10.4172/2329-9029.1000108

Cited by 23 publications

(9 citation statements)

References 29 publications

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“…Various secondary metabolites are modulated by abiotic stresses and play crucial role in stress tolerance/adaptation (Jain 2013 Fig. 6 Transcriptional regulation of metabolic pathways enriched during different abiotic stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Genome-Scale Transcriptomic Insights into Molecular Aspects of Abiotic Stress Responses in Chickpea

2014

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Chickpea is an important legume crop plant and various abiotic stresses are the major constraints affecting its overall productivity. For discovery of candidate genes involved in abiotic stress responses, we employed RNA sequencing for transcriptome profiling of roots and shoots of chickpea seedlings subjected to desiccation, salinity, and cold stresses. In total, we generated more than 250 million highquality reads from non-stressed and stressed tissue samples. Data analyses provided a comprehensive view of the dynamic transcriptional response of chickpea tissues to different abiotic stresses. Differential expression analysis identified a total of 11,640 chickpea transcripts showing response to at least one of the stress conditions. The reference-based transcriptome assembly was generated and at least 3,536 previously unannotated gene loci differentially expressed under abiotic stress conditions were identified. We observed extensive transcriptional reprogramming of genes involved in transcription regulation, energy metabolism, photosynthesis, hormonal responses, secondary metabolite biosynthesis and osmoprotectant metabolism under stress conditions. In addition, genes involved in post-translational modifications, RNA metabolic processes, and epigenetic regulation were also significantly highlighted. The comprehensive transcriptome analyses presented in this study revealed several potential key regulators of plant response to abiotic stresses and open avenues to carry out functional and applied genomic studies for improving abiotic stress tolerance in chickpea.

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Section: Discussionmentioning

confidence: 99%

Genome-Scale Transcriptomic Insights into Molecular Aspects of Abiotic Stress Responses in Chickpea

2014

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“…Succinic acid, trehalose and aspartic acid (aspartate) were selected for further genome analysis due to their potential involvement in biochemical pathways that were linked to the drought stress-specific responses [ 49 , 50 ]. The primary focus for genomic analysis was drought stress tolerant genotypes, most specifically TR39477 due to the increased level of succinic acid.…”

Section: Discussionmentioning

confidence: 99%

Comparative metabolite profiling of drought stress in roots and leaves of seven Triticeae species

et al. 2017

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BackgroundDrought is a lifestyle disease. Plant metabolomics has been exercised for understanding the fine-tuning of the potential pathways to surmount the adverse effects of drought stress. A broad spectrum of morphological and metabolic responses from seven Triticeae species including wild types with different drought tolerance/susceptibility level was investigated under control and water scarcity conditions.ResultsSignificant morphological parameters measured were root length, surface area, average root diameter and overall root development. Principal Component Analysis, Partial Least-Squares-Discriminant Analysis and Hierarchical Cluster Analysis were applied to the metabolomic data obtained by Gas Chromatography-Mass Spectrometry technique in order to determine the important metabolites of the drought tolerance across seven different Triticeae species. The metabolites showing significant accumulation under the drought stress were considered as the key metabolites and correlated with potential biochemical pathways, enzymes or gene locations for a better understanding of the tolerance mechanisms. In all tested species, 45 significantly active metabolites with possible roles in drought stress were identified. Twenty-one metabolites out of forty-five including sugars, amino acids, organic acids and low molecular weight compounds increased in both leaf and root samples of TR39477, IG132864 and Bolal under the drought stress, contrasting to TTD-22, Tosunbey, Ligustica and Meyeri samples. Three metabolites including succinate, aspartate and trehalose were selected for further genome analysis due to their increased levels in TR39477, IG132864, and Bolal upon drought stress treatment as well as their significant role in energy producing biochemical pathways.ConclusionThese results demonstrated that the genotypes with high drought tolerance skills, especially wild emmer wheat, have a great potential to be a genetic model system for experiments aiming to validate metabolomics–genomics networks.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4321-2) contains supplementary material, which is available to authorized users.

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“…Osmoprotectants containig ammonium compounds (polyamines, glycinebetaine, b-alanine betaine, dimethyl-sulfonio propionate and choline-Osulfate), osmoprotectants containing sugars and sugar alcohols (trehalose, fructan, mannitol and D-ononitol and sorbitol) and osmoprotectants containing amino acids (proline and ectoine). The manipulation in osmoprotectants responsive genes is one of the strategies to improve stress tolerance in plants by enhancing their production (Reguera et al 2012;Jain 2013). In this review, we discuss mechanisms how osmoprotectants or osmolytes are involved in plant defence mechanisms under salinity and drought stress conditions.…”

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Roles of osmoprotectants in improving salinity and drought tolerance in plants: a review

Singh

Kumar

Singh

et al. 2015

Rev Environ Sci Biotechnol

524

259

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Abiotic stresses collectively are responsible for crop losses worldwide. Among various abiotic stresses, drought and salinity are the most destructive. Different strategies have been adopted for the management of these stresses. Being complex traits, conventional breeding approaches have shown less success in improving salinity and drought stress tolerance. Roles of compatible solutes in salinity and drought stress tolerance have been studied extensively. At physiological level, osmotic adjustment is an adaptive mechanism involved in drought and/or salinity tolerance and permits the maintenance of turgor pressure under stress conditions. Increasing evidences from series of in vivo and in vitro studies involving physiological, biochemical, genetic, and molecular approaches strongly suggest that osmolytes such as ammonium compounds (polyamines, glycinebetaine, b-alanine betaine, dimethyl-sulfonio propionate and choline-O-sulfate), sugars and sugar alcohols (fructan, trehalose, mannitol, D-ononitol and sorbitol) and amino acids (proline and ectoine) perform important function in adjustment of plants against salinity and drought stresses. Thus, aim of this review is to expose how to osmoprotectants detoxify adverse effect of reactive oxygen species and alleviate drought and salinity stresses. An understanding of the relationship between these two sets of parameters is needed to develop measures for mitigating the damaging impacts of salinity and drought stresses.

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Emerging Role of Metabolic Pathways in Abiotic Stress Tolerance

Cited by 23 publications

References 29 publications

Genome-Scale Transcriptomic Insights into Molecular Aspects of Abiotic Stress Responses in Chickpea

Genome-Scale Transcriptomic Insights into Molecular Aspects of Abiotic Stress Responses in Chickpea

Comparative metabolite profiling of drought stress in roots and leaves of seven Triticeae species

Roles of osmoprotectants in improving salinity and drought tolerance in plants: a review

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