Emerging Pathogenic Gammaproteobacteria Wohlfahrtiimonas chitiniclastica and Ignatzschineria Species in a Turkey Vulture (Cathartes aura)

Oakley, Brian B.; Melgarejo, Tonatiuh; Bloom, Peter H.; Abedi, Nairi; Blumhagen, Emalee; Saggese, Miguel D.

doi:10.1647/19-00033

Cited by 3 publications

(3 citation statements)

References 60 publications

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“…W . chitiniclastica has been recognized as an emerging zoonotic pathogen by the United States Centers for Disease Control and Prevention (CDC) [ 84 ]. Kopf et al suggested the use of trimethoprim/sulfamethoxazole, levofloxacin, and cephalosporins (e.g., ceftazidime) antibiotics for W .…”

Section: Discussionmentioning

confidence: 99%

Multi-drug resistant (MDR) Gram-negative pathogenic bacteria isolated from poultry in the Noakhali region of Bangladesh

Munim,

Das,

Hossain

et al. 2024

PLoS ONE

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Rapidly increasing antibiotic-resistant bacterial strains in Bangladesh’s food and farm animals stem from the excessive and inappropriate use of antibiotics. To assess the prevalence of multi-drug resistant (MDR) Gram-negative bacteria in poultry chicks, we sought to isolate and identify strains carrying antimicrobial resistance genes. Isolation and identification involved biochemical tests, 16S rRNA sequencing, and PCR screening of species-specific genes. MDR patterns were evaluated using CLSI guidelines with seventeen antibiotics across twelve classes. Targeted gene sequences were amplified for the detection of Extended-spectrum β-Lactamase (ESBL), carbapenem, tetracycline, sulfonamide, and colistin resistance genes. Common isolates, such as Escherichia coli, Klebsiella pneumoniae, Proteus penneri, and Enterobacter hormaechei, exhibited average Multiple Antimicrobial Resistance (MAR) indices of 0.66, 0.76, 0.8, 0.84, and 0.81, 0.76, 0.84, 0.41 for broiler and layer chicken, respectively. Providencia stuartii and Salmonella enterica, exclusive to broiler samples, had MAR indices of 0.82 and 0.84, respectively. Additional isolates Morganella morganii, Aeromonas spp., and Wohlfahrtiimonas chitiniclastica were found in layers (Average MAR indices: 0.73, 0.71, and 0.91). Notably, M. morganii, E. hormaechei and W. chitiniclastica were identified for the first time in Bangladeshi poultry chicken, although their evolution is yet to be understood. In this study, Pan-drug resistance was observed in one P. stuartii (broiler) and one Aeromonas spp. (layer) with a MAR index 1, while all isolates exhibited MAR indices >0.2, indicating MDR. Antimicrobial resistance (AMR) gene screening identified blaTEM, blaSHV, tetA, and sul1 in a majority of the MDR strains. Interestingly, E. coli (lactose positive and negative) and E. hormaechei were exclusively found to possess the tetB gene. In addition, E. coli (lactose negative), Klebsiella pneumoniae, Enterobacter hormaechei, M. morganii, and P. stuartii were observed to carry the colistin-resistant mcr-1 gene, whereas sul2 was detected in E. coli (lactose positive and negative), E. hormaechei, P. stuartii, and P. penneri. These findings emphasize the health risk of our consumers of both broiler and layer chickens as they have turned into a potent reservoir of various AMR gene carrying MDR and Pan-drug resistant bacteria.

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Section: Discussionmentioning

confidence: 99%

Multi-drug resistant (MDR) Gram-negative pathogenic bacteria isolated from poultry in the Noakhali region of Bangladesh

Munim,

Das,

Hossain

et al. 2024

PLoS ONE

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“…Moreover, 50 % of these isolates showed intermediate effects towards aztreonam. W. chitiniclastica has been recognized as an emerging zoonotic pathogen by the United States Centers for Disease Control and Prevention (CDC) (83). Kopf et al suggested the use of trimethoprim/sulfamethoxazole, levofloxacin, and cephalosporins (e.g., ceftazidime) antibiotics for W. chitiniclastica infections (64).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, 50 % of these isolates showed intermediate effects towards aztreonam. W. chitiniclastica has been recognized as an emerging zoonotic pathogen by the United States Centers for Disease Control and Prevention (CDC)(83). %), ampicillin (10 %), and gentamicin (20 %), while being sensitive to streptomycin (30 %), chloramphenicol (65 %), ciprofloxacin (18 %), norfloxacin (83 %), nalidixic acid (85 %), tetracycline…”

mentioning

confidence: 99%

Unveiling multi-drug resistant (MDR) gram negative pathogenic bacteria from poultry chickens in the Noakhali region of Bangladesh

Munim,

Das,

Hossain

et al. 2023

Preprint

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Rapidly increasing antibiotic-resistant bacterial strains in Bangladesh’s food and farm animals stem from the excessive and inappropriate use of antibiotics. To assess the prevalence of multi drug resistant (MDR) gram-negative bacteria in poultry chicks, we sought to isolate and identify strains carrying antimicrobial resistance genes. Isolation and identification involved biochemical tests,16SrRNA sequencing, and PCR screening of species-specific genes. MDR patterns were evaluated using CLSI guidelines with seventeen antibiotics across twelve classes. Targeted gene sequences were amplified for detection of ESBL, carbapenem, tetracycline, sulfonamide, and colistin resistance genes. Common isolates, such asEscherichia coli,Klebsiella pneumoniae, Proteus penneri,andEnterobacter hormaechei, exhibited average Multiple Antimicrobial Resistance (MAR) indices of 0.66, 0.76, 0.8, 0.84, and 0.81, 0.76, 0.84, 0.41 for broiler and layer chicken, respectively.Providencia stuartiiandSalmonella enterica, exclusive to broiler samples, had MAR indices of 0.82 and 0.84, respectively. Additional isolatesMorganella morganii,Aeromonasspp., andWohlfahrtiimonas chitiniclasticawere found in layers (Average MAR indices: 0.73, 0.71, and 0.91). Notably,M. morganii, E. hormaechei and W. chitiniclasticawere identified for the first time in Bangladeshi poultry chicken, although their evolution is yet to understand. In this study, Pan-drug resistance was observed in oneP. stuartii(broiler) and oneAeromonasspp. (layer) with a MAR index 1, while all isolates exhibited MAR indices >0.2, indicating MDR. Antimicrobial resistance (AMR) gene screening identifiedblaTEM,blaSHV,tetA, andsul1in a majority of the MDR strains. Interestingly,E. coli(lactose positive and negative) andE. hormaecheiwere exclusively found to possess thetetBgene. In addition,E. coli(lactose negative),Klebsiella pneumoniae,Enterobacter hormaechei,M. morganii, andP. stuartiiwere observed to carry the colistin-resistantmcr1gene, whereassul2was detected inE. coli(lactose positive and negative),E. hormaechei,P. stuartii, andP. penneri. These findings emphasize the health risk of our consumers of both broiler and layer chickens as they have turned to a potent reservoir of various AMR gene carrying MDR and Pan-drug resistant bacteria.

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Phylogenomics and comparative genomic analyses support the creation of the novel family Ignatzschineriaceae fam. nov. comprising the genera Ignatzschineria and Wohlfahrtiimonas within the order Cardiobacteriales

Montecillo

2023

Research in Microbiology

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Emerging Pathogenic Gammaproteobacteria Wohlfahrtiimonas chitiniclastica and Ignatzschineria Species in a Turkey Vulture (Cathartes aura)

Cited by 3 publications

References 60 publications

Multi-drug resistant (MDR) Gram-negative pathogenic bacteria isolated from poultry in the Noakhali region of Bangladesh

Multi-drug resistant (MDR) Gram-negative pathogenic bacteria isolated from poultry in the Noakhali region of Bangladesh

Unveiling multi-drug resistant (MDR) gram negative pathogenic bacteria from poultry chickens in the Noakhali region of Bangladesh

Phylogenomics and comparative genomic analyses support the creation of the novel family Ignatzschineriaceae fam. nov. comprising the genera Ignatzschineria and Wohlfahrtiimonas within the order Cardiobacteriales

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