Emerging of microRNAs as Key Regulators in Plant Secondary Metabolism

Samad, Abdul Fatah A.; Sajad, Muhammad; Ismail, Ismanizan

doi:10.1007/978-3-030-35772-6_7

Cited by 3 publications

(3 citation statements)

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“…miR828 is also involved in the regulation of trichome and cotton fiber development by targeting MYB2 in Arabidopsis ( Guan et al, 2014 ), as well as anthocyanin and flavonol accumulation by targeting MYB114 in grape ( Tirumalai et al, 2019 ). As the target of miR828, MYB modulate gene expression patterns in the flavonoid biosynthetic pathway ( Samad et al, 2020 ). MYB94 formed a network with LCY-b in terpenoid pathway and HSF4 targeted by miR1134, which is involved in the terpenoid biosynthetic pathway by miRNA-based regulation ( Fan et al, 2015 ; Samad et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…As the target of miR828, MYB modulate gene expression patterns in the flavonoid biosynthetic pathway ( Samad et al, 2020 ). MYB94 formed a network with LCY-b in terpenoid pathway and HSF4 targeted by miR1134, which is involved in the terpenoid biosynthetic pathway by miRNA-based regulation ( Fan et al, 2015 ; Samad et al, 2020 ). The expression of genes in the flavonoid and terpenoid biosynthetic pathways was altered in periwinkle in response to CLas infection ( Supplementary Tables 6 , 7 ).…”

Section: Discussionmentioning

confidence: 99%

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Integrated Analysis of the miRNAome and Transcriptome Reveals miRNA–mRNA Regulatory Networks in Catharanthus roseus Through Cuscuta campestris-Mediated Infection With “Candidatus Liberibacter asiaticus”

Zeng

Cao

et al. 2022

Front. Microbiol.

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Citrus Huanglongbing (HLB) is the most devastating disease of citrus caused by the Gram-negative phloem-limited bacterium “Candidatus Liberibacter asiaticus” (CLas). It can be transmitted by the Asian citrus psyllid “Diaphorina citri,” by grafting, and by the holoparasitic dodder. In this study, the non-natural host periwinkle (Catharanthus roseus) was infected via dodder (Cuscuta campestris) from CLas-infected citrus plants, and the asymptomatic leaves (AS) were subjected to transcriptomic and small-RNA profiling. The results were analyzed together with a transcriptome dataset from the NCBI repository that included leaves for which symptoms had just occurred (S) and yellowing leaves (Y). There were 3,675 differentially expressed genes (DEGs) identified in AS, and 6,390 more DEGs in S and further 2109 DEGs in Y. These DEGs were commonly enriched in photosystem, chloroplast, membrane, oxidation-reduction process, metal/zinc ion binding on GO. A total of 14,974 DEGs and 336 DE miRNAs (30 conserved and 301 novel) were identified. Through weighted gene co-expression network and nested network analyses, two critical nested miRNA–mRNA regulatory networks were identified with four conserved miRNAs. The primary miR164-NAC1 network is potentially involved in plant defense responses against CLas from the early infection stage to symptom development. The secondary network revealed the regulation of secondary metabolism and nutrient homeostasis through miR828-MYB94/miR1134-HSF4 and miR827-ATG8 regulatory networks, respectively. The findings discovered new potential mechanisms in periwinkle–CLas interactions, and its confirmation can be done in citrus–CLas system later on. The advantages of periwinkle plants in facilitating the quick establishment and greater multiplication of CLas, and shortening latency for disease symptom development make it a great surrogate for further studies, which could expedite our understanding of CLas pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of the miRNAome and Transcriptome Reveals miRNA–mRNA Regulatory Networks in Catharanthus roseus Through Cuscuta campestris-Mediated Infection With “Candidatus Liberibacter asiaticus”

Zeng

Cao

et al. 2022

Front. Microbiol.

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“…The miR156/SPL module is associated with leaf maturation, trichome formation, male fertility (anther development), phytomer length and composition, glucose and trehalose metabolism, as well as stress responses (Wang et al ., 2009; Xing et al ., 2010; Yu et al ., 2010; Poethig, 2013; Fouracre & Poethig, 2016; Silva et al ., 2019; Lawrence et al ., 2020). Interestingly, several miRNA regulatory modules have been shown to be important for controlling the biosynthesis of secondary metabolites, including the miR156/SPL module (Padhan et al ., 2016; Samad et al ., 2020). At present, few examples connect the miR156-targeted and non-targeted SPLs with the control of the secondary metabolism.…”

Section: Introductionmentioning

confidence: 99%

Plant age-dependent dynamics of annatto pigment (bixin) biosynthesis inBixa orellanaL.

Machado

Faria

Duarte

et al. 2022

Preprint

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Age affects the production of secondary metabolites, but how developmental cues regulate secondary metabolism remains poorly understood. Annatto (Bixa orellana L.) is a source of bixin, an apocarotenoid used in the world's food industry worldwide. Understanding how age-dependent mechanisms control bixin biosynthesis is of great interest for plant biology and for pharmaceutical, cosmetic, and textile industries. Here, we used genetic and molecular tools to unravel the role of the annatto age regulated miRNA156 (miR156) targeted SQUAMOSA PROMOTER BINDING PROTEIN LIKE (BoSPL) genes in secondary metabolism. Low expression of several BoSPL genes in miR156 overexpressing annatto plants (OE::156) impacted leaf ontogeny, reducing bixin production and increasing abscisic acid (ABA) levels. Modulation of BoCCD4;4 and BoCCD1 expression, key genes in lycopene cleavage, was associated with diverting the carbon flux from bixin to ABA, whereas upregulation of lycopene β cyclase genes implies the xanthophyll biosynthetic pathway acted as a carbon sink in OE::156 plants. Proteomic analyses revealed low accumulation of most secondary metabolite-related enzymes in OE::156 plants, suggesting that miR156 targeted BoSPLs are required to activate several annatto secondary metabolic pathways. Our findings suggest that carbon flux in B. orellana OE::156 leaves was redirected from bixin to ABA production, indicating an age-dependent leaf dynamics of bixin biosynthesis. Importantly, our study opened a new venue to future annatto breeding programs aiming to improve bixin output.

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Plant age-dependent dynamics of annatto pigment (bixin) biosynthesis in Bixa orellana

Machado,

Faria,

Duarte

et al. 2023

Journal of Experimental Botany

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Age affects the production of secondary metabolites, but how developmental cues regulate secondary metabolism remains poorly understood. The achiote tree (Bixa orellana L.) is a source of bixin, an apocarotenoid used in diverse industries worldwide. Understanding how age-dependent mechanisms control bixin biosynthesis is of great interest for plant biology and for economic reasons. Here we overexpressed miRNA156 (miR156) in B. orellana to comprehensively study the effects of the miR156/SQUAMOSA PROMOTER BINDING PROTEIN–LIKE (SPL) module on the age-dependent bixin biosynthesis in leaves. The overexpression of miR156 in annatto plants (miR156ox) reduced BoSPL transcript levels, impacted leaf ontogeny, lessened bixin production, and increased abscisic acid (ABA) levels. Modulation of BoCCD4-4 and BoCCD1 expression, key genes in carotenoid biosynthesis, was associated with diverting the carbon flux from bixin to ABA in miR156ox leaves. Proteomic analyses revealed an overall low accumulation of most secondary metabolite-related enzymes in miR156ox leaves, suggesting that miR156-targeted BoSPLs may be required to activate several secondary metabolic pathways. Our findings suggest that the conserved BomiR156/BoSPL module is deployed to regulate leaf dynamics of bixin biosynthesis, and may create novel opportunities to fine-tune bixin output in B. orellana breeding programs.

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Emerging of microRNAs as Key Regulators in Plant Secondary Metabolism

Cited by 3 publications

References 54 publications

Integrated Analysis of the miRNAome and Transcriptome Reveals miRNA–mRNA Regulatory Networks in Catharanthus roseus Through Cuscuta campestris-Mediated Infection With “Candidatus Liberibacter asiaticus”

Integrated Analysis of the miRNAome and Transcriptome Reveals miRNA–mRNA Regulatory Networks in Catharanthus roseus Through Cuscuta campestris-Mediated Infection With “Candidatus Liberibacter asiaticus”

Plant age-dependent dynamics of annatto pigment (bixin) biosynthesis inBixa orellanaL.

Plant age-dependent dynamics of annatto pigment (bixin) biosynthesis in Bixa orellana

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