Emerging nanoproteomics approaches for disease biomarker detection: A current perspective

S, Ray; Reddy, Panga Jaipal; Choudhary, Sandeep; Raghu, Dinesh; Srivastava, Sanjeeva

doi:10.1016/j.jprot.2011.04.027

Cited by 61 publications

(42 citation statements)

References 194 publications

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“…The non-specific adsorption of proteins on the exposed surfaces of the microfluidic channels is essential as regards bioanalytical tasks demanding high sensitivity [36]. To check our surface modified PDMS from this point of view, the adsorption of fluorescein-isothiocyanate (FITC) labelled HSA on the exposed channel surface was recorded by fluorescent microscopy.…”

Section: Effect Of Surface Modification On Protein Bindingmentioning

confidence: 99%

PDMS microfluidics developed for polymer based photonic biosensors

et al. 2014

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Abstract:In this work, advances in the fabrication technology and functional analysis of a polymer microfluidic system -as a significant part of a developed polymer photonic biosensor -are reported. Robust and cost-effective microfluidics in PDMS including sample preparation functions is designed and realized by using SU-8 moulding replica. Surface modification strategies using Triton X-100 and PDMS-PEO and their effect on device sealing and non-specific protein adsorption are investigated by contact angle measurement and in situ fluorescence microscopy.Response to Reviewers: Dear Editors and Reviewers, First of all, thank You for the effort in improving our paper. Regarding the reviewer comments, we made the following modifications regarding the latest version of the manuscript submitted. The modifications and the required supplements are submitted in pdf format with the revised manuscript.Thank You again for the efforts to improve our manuscript. Regards, Péter Fürjes

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Section: Effect Of Surface Modification On Protein Bindingmentioning

confidence: 99%

PDMS microfluidics developed for polymer based photonic biosensors

et al. 2014

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“…In this regard, nanoproteomics are seen to be functioning as protein amplification techniques similar to PCR. Potential clinical applications include infectious, endocrine, autoimmune, and neurodegenerative diseases, as well as brain injury and several types of tumors (Ray et al, 2011). Some of these applications are illustrated in Table 1 and Figure 4.…”

Section: Nanoproteomics In Clinical Applicationsmentioning

confidence: 99%

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Kobeissy

Gülbakan

Alawieh

et al. 2014

OMICS: A Journal of Integrative Biology

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The post-genomics era has brought about new Omics biotechnologies, such as proteomics and metabolomics, as well as their novel applications to personal genomics and the quantified self. These advances are now also catalyzing other and newer post-genomics innovations, leading to convergences between Omics and nanotechnology. In this work, we systematically contextualize and exemplify an emerging strand of post-genomics life sciences, namely, nanoproteomics and its applications in health and integrative biological systems. Nanotechnology has been utilized as a complementary component to revolutionize proteomics through different kinds of nanotechnology applications, including nanoporous structures, functionalized nanoparticles, quantum dots, and polymeric nanostructures. Those applications, though still in their infancy, have led to several highly sensitive diagnostics and new methods of drug delivery and targeted therapy for clinical use. The present article differs from previous analyses of nanoproteomics in that it offers an in-depth and comparative evaluation of the attendant biotechnology portfolio and their applications as seen through the lens of post-genomics life sciences and biomedicine. These include: (1) immunosensors for inflammatory, pathogenic, and autoimmune markers for infectious and autoimmune diseases, (2) amplified immunoassays for detection of cancer biomarkers, and (3) methods for targeted therapy and automatically adjusted drug delivery such as in experimental stroke and brain injury studies. As nanoproteomics becomes available both to the clinician at the bedside and the citizens who are increasingly interested in access to novel post-genomics diagnostics through initiatives such as the quantified self, we anticipate further breakthroughs in personalized and targeted medicine.

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“…Detection and quantification are often fluorescence-based and identify interactions between proteins, kinase substrates, activators of transcription factors [127]. Nanoproteomics has the potential to provide fast, highthroughput and sensitive methods using only minute amount of samples [128]. However, MS is currently the main technique for large-scale whole-proteome study with precise measurements [129,130].…”

Section: Proteomicsmentioning

confidence: 99%

Functional Genomics, Proteomics, Metabolomics and Bioinformatics for Systems Biology

et al. 2013

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This chapter introduces Systems Biology, its context, aims, concepts and strategies, then describes approaches used in genomics, epigenomics, transcriptomics, proteomics, metabolomics and lipidomics, and how recent technological advances in these fields have moved the bottleneck from data production to data analysis. Methods for clustering, feature selection, prediction analysis, text mining and pathway analysis used to analyse and integrate the data produced are then presented.

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Emerging nanoproteomics approaches for disease biomarker detection: A current perspective

Cited by 61 publications

References 194 publications

PDMS microfluidics developed for polymer based photonic biosensors

PDMS microfluidics developed for polymer based photonic biosensors

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Functional Genomics, Proteomics, Metabolomics and Bioinformatics for Systems Biology

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