Emerging Methods to Generate Artificial Germ Cells from Stem Cells1

Zeng, Fanhui; Huang, Fajun; Guo, Jingjing; Xing-chang, HU; Liu, Changbai; Wang, Hu

doi:10.1095/biolreprod.114.124800

Cited by 18 publications

(15 citation statements)

References 156 publications

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“…Consequently, the combination of niche reprogramming and newly found 3D microenvironment may be useful for stem cell-based artificial germ cell generation. 32…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, the combination of niche reprogramming and newly found 3D microenvironment may be useful for stem cell-based artificial germ cell generation. 32 Although various efforts have been made for the translation of research experiences on tissue engineering especially stem cell differentiation into germ cell, 20,33 proliferation and differentiation of stem cells in 2D culture has remained highly challenging because of technical demands encountered with acquisition of appropriate stem cells and also preparing suitable scaffold supporting stem cell differentiation. Besides biocompatibility, a scaffold should match biomechanical characteristics of the cells and be able to properly support cells during proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

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Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

et al. 2017

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Infertility caused by the disruption or absence of germ cells is a major and largely incurable medical problem. Germ cells (i.e., sperm or egg) play a key role in the transmission of genetic and epigenetic information across generations. Generation of gametes derived in vitro from stem cells hold promising prospects which could potentially help infertile men and women. Menstrual blood-derived stem cells are a unique stem cell source. Evidence suggests that menstrual blood-derived stem cells exhibit a multi-lineage potential and have attracted extensive attention in regenerative medicine. To maintain the three-dimensional structure of natural extra cellular matrices in vitro, scaffolds can do this favor and mimic a microenvironment for cell proliferation and differentiation. According to previous studies, poly(lactic acid) and multi-wall carbon nanotubes have been introduced as novel and promising biomaterials for the proliferation and differentiation of stem cells. Some cell types have been successfully grown on a matrix containing carbon nanotubes in tissue engineering but there is no report for this material to support stem cells differentiation into germ cells lineage. This study designed a 3D wet-electrospun poly(lactic acid) and poly(lactic acid)/multi-wall carbon nanotubes composite scaffold to compare infiltration, proliferation, and differentiation potential of menstrual blood-derived stem cells toward germ cell lineage with 2D culture. Our primary data revealed that the fabricated scaffold has mechanical and biological suitable qualities for supporting and attachments of stem cells. The differentiated menstrual blood-derived stem cells tracking in scaffolds using scanning electron microscopy confirmed cell attachment, aggregation, and distribution on the porous scaffold. Based on the differentiation assay by RT-PCR analysis, stem cells and germ-like cells markers were expressed in 3D groups as well as 2D one. It seems that poly(lactic acid)/multi-wall carbon nanotubes scaffold-seeded menstrual blood-derived stem cells could be viewed as a novel, safe, and accessible construct for these cells, as they enhance germ-like generation from menstrual blood-derived stem cells.

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“…Consequently, the combination of niche reprogramming and newly found 3D microenvironment may be useful for stem cell-based artificial germ cell generation. 32…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

et al. 2017

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“…Accordingly, CPP have demonstrated utility as valuable vehicle for intracellular delivery of macromolecules, cargo has to be covalently [13] or non-covalently [14] linked to CPPs for efficient uptake. In recent years, numerous applications for CPPs not only have been shown from a vast number of fundamental researches (including CPP-based somatic cell reprogramming [5, 15–18], CPP-mediated genome editing [5, 19–21]), but also a little number of CPP-coupled molecules has entered into a phase II clinical trials [6]. …”

Section: Introductionmentioning

confidence: 99%

Efficient therapeutic delivery by a novel cell-permeant peptide derived from KDM4A protein for antitumor and antifibrosis

Wang

Yang

et al. 2016

Oncotarget

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Cell-penetrating peptide (CPP) based delivery have provided immense potential for the therapeutic applications, however, most of nonhuman originated CPPs carry the risk of possible cytotoxicity and immunogenicity, thus may restricting to be used. Here, we describe a novel human-derived CPP, denoted hPP10, and hPP10 has cell-penetrating properties evaluated by CellPPD web server, as well as In-Vitro and In-Vivo analysis. In vitro studies showed that hPP10-FITC was able to penetrate into various cells including primary cultured cells, likely through an endocytosis pathway. And functionalized macromolecules, such as green fluorescent protein (GFP), tumor-specific apoptosis inducer Apoptin as well as biological active enzyme GCLC (Glutamate-cysteine ligase, catalytic subunit) can be delivered by hPP10 in vitro and in vivo. Collectively, our results suggest that hPP10 provide a novel and versatile tool to deliver exogenous proteins or drugs for clinical applications as well as reprogrammed cell-based therapy.

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“…Thus, either the injection of SSC into a naked testis of a recipient animal or the transplantation of SSC containing testis material somewhere in the recipient animal can lead to the sperm production. Note that SSC transplantations can be made with the use of differentiated stem cells from male PGC but also from differentiated stem cells derived from embryo's (ESC) as well as from induced pluripotent stem cells (iPSC) from somatic tissues (Aponte et al 2013;West et al 2013;Zeng et al 2015).…”

Section: Transfer Of Spermatogonia To Recipient Testismentioning

confidence: 99%

A Review of New Technologies that may Become Useful for in vitro Production of Boar Sperm

Gadella

Ferraz

2015

Reprod Domestic Animals

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Contents Making sperm cells outside the original testicular environment in a culture dish has been considered for a long time as impossible due to the very complicated process of spermatogenesis and sperm maturation, which altogether, encompasses a 2‐month period. However, new approaches in complex three‐dimensional co‐cell cultures, micro‐perfusion and micro‐fluidics technologies, new knowledge in the functioning, culturing and differentiation of spermatogonial stem cells (SSC) and their precursor cells have revolutionized this field. Furthermore, the use of better molecular markers as well as stimulatory factors has led to successful in vitro culture of stem cells either derived from germ line stem cells, from induced pluripotent stem cells (iPSC) or from embryonic stem cells (ESC). These stem cells when placed into small seminiferous tubule fragments are able to become SSC. The SSC beyond self‐renewal can also be induced into haploid sperm‐like cells under in vitro conditions. In mouse, this in vitro produced sperm can be injected into a mature oocyte and allow post‐fertilization development into an early embryo in vitro. After transferring such obtained embryos into the uterus of a recipient mouse, they can further develop into healthy offspring. Recently, a similar approach has been performed with combining selected cells from testicular cell suspensions followed by a complete in vitro culture of seminiferous cords producing sperm‐like cells. However, most of the techniques developed are laborious, time‐consuming and have low efficiency, placing questionable that it will become useful used for setting up an efficient in vitro sperm production system for the boar. The benefits and drawbacks as well as the likeliness of in vitro pig sperm production to become applied in assisted technologies for swine reproduction are critically discussed. In this contribution, also the process of sperm production in the testis and sperm maturation is reviewed.

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Emerging Methods to Generate Artificial Germ Cells from Stem Cells1

Cited by 18 publications

References 156 publications

Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

Efficient therapeutic delivery by a novel cell-permeant peptide derived from KDM4A protein for antitumor and antifibrosis

A Review of New Technologies that may Become Useful for in vitro Production of Boar Sperm

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