Emerging Low‐Dimensional Nanoagents for Bio‐Microimaging

Lin, Hongxin; Yang, Lijiao; Zhang, Xuan; Liu, Guoming; Zhuo, Shuangmu; Chen, Jianxin; Song, Jibin

doi:10.1002/adfm.202003147

Cited by 15 publications

(15 citation statements)

References 230 publications

(268 reference statements)

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“…CDs have shown excellent potential in the field of bioimaging due to the CDs have good dispersibility, the ability to specifically bind to target units, high near-infrared absorption and photoluminescence efficiency, good chemical stability and photostability ( Bondon et al, 2020 ; Lin et al, 2020 ; Cardoso Dos Santos et al, 2020 ; Wang et al, 2021 ). As known, Different types of cells have different structures and morphologies, and different cell membranes or cytoplasm contain different biomarkers, leading to specific responses to foreign nanoparticles.…”

Section: In Vitro Bioimagingmentioning

confidence: 99%

Recent Advances in Carbon Dots for In Vitro/Vivo Fluorescent Bioimaging: A Mini-Review

Lin

Mei

et al. 2022

Front. Chem.

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As a new type of “zero-dimensional” fluorescent carbon nanomaterials, carbon dots (CDs) have some unique optical and chemical properties, they are being explored for a variety of applications in bio-related fields, such as bioimaging, biosensors, and therapy. This review mainly summarizes the recent progress of CDs in bioimaging. The overview of this review can be roughly divided into two categories: (1) In vitro bioimaging based on CDs in different cells and important organelles. (2) The distribution, imaging and application of CDs in mice and zebrafish. In addition, this review also points out the potential advantages and future development directions of CDs for bioimaging, which may promote the development of CDs in the field of bioimaging.

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Section: In Vitro Bioimagingmentioning

confidence: 99%

Recent Advances in Carbon Dots for In Vitro/Vivo Fluorescent Bioimaging: A Mini-Review

Lin

Mei

et al. 2022

Front. Chem.

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“…Also, considering that the TPEF spectrum of many fluorescent molecules is wider than the single-photon excitation spectrum, it is possible to excite various fluorescent molecules with a single wavelength, thus obtaining more image information. Moreover, tissue absorption in the nearinfrared window (700-1,000 nm) is relatively small, giving TPEF a deeper penetration depth (4)(5)(6).…”

Section: Original Articlementioning

confidence: 99%

Three-dimensional characterizations of two-photon excitation fluorescence images of elastic fibers affected by cutaneous scar duration

Lin¹,

Lin²,

Zhu³

et al. 2021

Quant Imaging Med Surg

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Background: The type or duration of a scar determines the choice of therapy available. Traditional detection methods can easily cause secondary trauma, so there is an urgent need for a non-invasive, rapid diagnostic approach.Methods: A strategy for quantitative analysis of three-dimensional (3D) elastic fibers in human cutaneous scars was designed, which included 3D reconstruction, skeleton extraction, quantitative analysis, and random forest regression.Results: Four reconstruction methods were used to reconstruct 3D two-photon excitation fluorescence images of elastic fibers for comparison. In the skeleton extraction stage, the 3D thinning algorithm was improved to prepare for accurate quantitative analysis, in which eight parameters comprising branches number (B-NUM), nodes number (N-NUM), averaged branch broken-line length (AB-BL), averaged linear branch length (AB-LL), averaged branch tortuosity (AB-T), branch direction consistency (B-DC), averaged branch volume (AB-V), and averaged branch sectional area (AB-SA) were presented. Six of them, except averaged branch tortuosity (AB-T) and branch direction consistency (B-DC), showed an explicit tendency to change with scar duration. In the random forests regression analysis, the six extracted parameters could be used to predict scar duration with R 2 =0.981 and RMSE=0.513. Conclusions:The parameters we extracted had a distinct relationship with scar duration, and random forests regression showed better performance in forecasting scar duration than unitary models.

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“…Tumor visualization is an unabated topic in academia and clinic. [ 1 ] From the early blood test, to the traditional imaging examination, and finally to the gold standard of pathological diagnosis, the clinical diagnosis of tumor ultimately depends on the conclusion of visualization. [ 2 ] Traditional imaging relies on the visual observation of doctors in the imaging department, which leads to the frequent and easy neglect of small lesions.…”

Section: Introductionmentioning

confidence: 99%

“…Due to its unique advantages, such as higher spatial resolution, deeper tissue penetration, minimized photobleaching and lower damage to samples, two‐photon excitation fluorescence (TPEF) microscopy has become an optical imaging technology comparable to the gold standard of pathology. [ 1, 8 ] Numerous studies have shown that key markers of malignant tumors, such as GSH, are not uniformly distributed in this tumor region, making targeting difficult. [ 9, 10 ] At the same time, progress in the specific quantitative detection of GSH in vivo tumors have been very limited, especially in the TPEF imaging mode.…”

Section: Introductionmentioning

confidence: 99%

In‐vivo two‐photon visualization and quantitative detection of redox state of cancer

Wang

Guo-ming

et al. 2022

Journal of Biophotonics

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Glutathione (GSH), the most common and abundant antioxidant in the body, is particularly concentrated in cancer cells (2–10 mM). This concentration is approximately 1000 times that of normal cells, making GSH a specific tumor marker. Overexpression of GSH is critical for mapping the redox state of cancer cells. However, there are few probes and detection methods responsive to GSH that can quantitatively visualize GSH in vivo in two‐photon excitation fluorescence (TPEF) imaging mode. The experimental results show that TPEF‐GSH could not only target GSH in tumors, but also establish the quantitative relationship between TPEF signal and GSH concentration. We explored the optimal two‐photon excitation wavelength of TPEF‐GSH, the optimal cell incubation duration with TPEF‐GSH, the best imaging time point for GSH in cells, and the quantitative relationship between the TPEF signal and the changes in GSH concentrations. In zebrafish embryo and zebrafish experiments, the ratiometric value of TPEF‐GSH increased with the decrease of GSH concentration. Microinjection and co‐incubation were used to verify whether the ratiometric value could quantify endogenous GSH in tumor‐bearing zebrafish, and the obtained GSH levels were 4.66 mM and 5.16 mM, respectively. The ratio TPEF probe could accurately visualize and quantify GSH in vivo, reflecting the redox status of the tumor. The design of the ratiometric molecular probe provides a reliable strategy for the development of TPEF nanoprobe in vivo. In this article, a new GSH sensitive molecular probe, TPEF‐GSH, has been developed with good specificity and sensitivity. TPEF‐GSH was successfully used to image cancer cells in vitro and tumor‐bearing zebrafish in vivo, and to further detect GSH levels.

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Emerging Low‐Dimensional Nanoagents for Bio‐Microimaging

Cited by 15 publications

References 230 publications

Recent Advances in Carbon Dots for In Vitro/Vivo Fluorescent Bioimaging: A Mini-Review

Recent Advances in Carbon Dots for In Vitro/Vivo Fluorescent Bioimaging: A Mini-Review

Three-dimensional characterizations of two-photon excitation fluorescence images of elastic fibers affected by cutaneous scar duration

In‐vivo two‐photon visualization and quantitative detection of redox state of cancer

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