Emerging Insights into the Roles of the Paf1 Complex in Gene Regulation

Oss, S. Branden Van; Cucinotta, Christine E.; Arndt, Karen M.

doi:10.1016/j.tibs.2017.08.003

Cited by 143 publications

(140 citation statements)

References 93 publications

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“…Since the hPaf1 complex promotes transcription elongation and histone modifications associated with active transcription [105], E1A recruitment of the complex allows for efficient transcription of viral genes [104,106]. Since the hPaf1 complex promotes transcription elongation and histone modifications associated with active transcription [105], E1A recruitment of the complex allows for efficient transcription of viral genes [104,106].…”

Section: E1a Localizes To Host Chromatin Alters Host Histone Modificmentioning

confidence: 99%

“…On the viral genome, the interaction of hBre1 and its complex partner RNF20 recruits the human RNA polymerase II-associated factor 1 (hPaf1) complex to the viral DNA. Since the hPaf1 complex promotes transcription elongation and histone modifications associated with active transcription [105], E1A recruitment of the complex allows for efficient transcription of viral genes [104,106]. The targeting of hBre by E1A, which both downregulates the host viral defense mechanism and upregulates viral transcription, illustrates the economy of E1A's epigenetic dysregulation via host factors.…”

Section: E1a Localizes To Host Chromatin Alters Host Histone Modificmentioning

confidence: 99%

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Epigenetics and the dynamics of chromatin during adenovirus infections

et al. 2019

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Edited by Urs GreberAbbreviations ADP, adenovirus death protein; Ad5 or Ad 12, human adenovirus type 5 or 12, or other type referenced; BHK, baby hamster kidney; BrdU, bromodeoxyuridine; CTCF, CCCTC binding factor; DBS, double stranded break; DDR, DNA damage response; E1A, early 1 A; E4orf3 or E4orf6, early 4 open reading frame 3 or 6; HAdV-A12, human adenovirus subgroup A type 12, or different subgroup or type referenced; HMGB, high-mobility group box; hPaf1, human RNA polymerase II-associated factor 1; ISGs, interferon-stimulated genes; PML, promyelocytic leukemia; snRNP, small nuclear ribonuclear protein; SET or TAF-1, SE translocation or template activating factor; VRCs, viral replication centers.

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Section: E1a Localizes To Host Chromatin Alters Host Histone Modificmentioning

confidence: 99%

Section: E1a Localizes To Host Chromatin Alters Host Histone Modificmentioning

confidence: 99%

Epigenetics and the dynamics of chromatin during adenovirus infections

et al. 2019

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“…43:239-244. B. Schematic picture of high-confidence interactions from BioID for nuclear actin that are involved in different stages of transcription (AJ et al, 2016;Gruber et al, 2014;Wahl and Luhrmann, 2015;Van Oss et al, 2017). Compositions of general transcription factors (GTFs) were obtained from KEGG pathway Ko03022 (Kanehisa and Goto, 2000).…”

Section: Statistical Analysesmentioning

confidence: 99%

Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing

Viita

Kyheröinen

Prajapati

et al. 2018

Preprint

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In addition to its essential functions within the cytoskeleton, actin also localizes to the cell nucleus, where it is linked to many important nuclear processes from gene expression to maintenance of genomic integrity. However, the molecular mechanisms by which actin operates in the nucleus remain poorly understood. Here we have used two complementary mass spectrometry (MS) techniques, AP-MS and BioID-MS, to identify binding partners for nuclear actin. Common highconfidence interactions highlight the role of actin in chromatin remodeling complexes and identify the hATAC histone modifier as a novel actin-containing nuclear complex. Further analysis demonstrates that actin binds directly to the hATAC subunit KAT14, and modulates its histone acetyl transferase activity in vitro and in cells. BioID-MS, which can detect also transient interactions, links actin to several steps of transcription as well as to RNA processing. Alterations in nuclear actin levels disturb alternative exon skipping of the SMN2 minigene, suggesting also a functional role for actin in RNA splicing. This interactome analysis thus identifies both novel direct binding partners and functional roles for nuclear actin, as well as forms a platform for further mechanistic studies on how actin operates during essential nuclear processes.

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“…The E2 ubiquitin-conjugating enzyme Rad6 catalyzes H2B monoubiquitination in conjunction with the E3 ubiquitin ligase Bre1 and the transcriptional regulatory protein Lge1 [8][9][10][11]. H2Bub1 is present throughout the entire transcriptional units of active genes due to the association of Rad6 and Bre1 with chromatin through their interaction with activators and with RNA polymerase II (RNAPII) [12,13]. H2B is, therefore, cotranscriptionally ubiquitinated, and its H2Bub1 levels correlate with RNAPII elongation rates [14].…”

Section: Introductionmentioning

confidence: 99%

A role for Mog1 in H2Bub1 and H3K4me3 regulation affecting RNAPII transcription and mRNA export

et al. 2018

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Monoubiquitination of histone H2B (to H2Bub1) is required for downstream events including histone H3 methylation, transcription, and mRNA export. The mechanisms and players regulating these events have not yet been completely delineated. Here, we show that the conserved Ran‐binding protein Mog1 is required to sustain normal levels of H2Bub1 and H3K4me3 in Saccharomyces cerevisiae. Mog1 is needed for gene body recruitment of Rad6, Bre1, and Rtf1 that are involved in H2B ubiquitination and genetically interacts with these factors. We provide evidence that the absence of MOG1 impacts on cellular processes such as transcription, DNA replication, and mRNA export, which are linked to H2Bub1. Importantly, the mRNA export defect in mog1Δ strains is exacerbated by the absence of factors that decrease H2Bub1 levels. Consistent with a role in sustaining H2Bub and H3K4me3 levels, Mog1 co‐precipitates with components that participate in these modifications such as Bre1, Rtf1, and the COMPASS‐associated factors Shg1 and Sdc1. These results reveal a novel role for Mog1 in H2B ubiquitination, transcription, and mRNA biogenesis.

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Emerging Insights into the Roles of the Paf1 Complex in Gene Regulation

Cited by 143 publications

References 93 publications

Epigenetics and the dynamics of chromatin during adenovirus infections

Epigenetics and the dynamics of chromatin during adenovirus infections

Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing

A role for Mog1 in H2Bub1 and H3K4me3 regulation affecting RNAPII transcription and mRNA export

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