Emerging Frontiers of Microbes as Agro-Waste Recycler

Rai, Shalini; Solanki, Manoj Kumar; Anal, Ajit Kumar Dubedi; Sagar, Alka; Solanki, Anjali Chandrol; Kashyap, Brijendra Kumar; Pandey, Akhilesh Kumar

doi:10.1007/978-981-33-4347-4_1

Cited by 14 publications

(2 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is plenty of waste produced from forests, farm lands, and agro-industries. These wastes have maximum amount of cellulose which is discarded or underused, resulting pollution in the atmosphere [274,275]. Researchers have shown that characterization of cellulase producing bacteria and their genome functional analyses help to improve waste management [276].…”

Section: Cellulase In Waste Managementmentioning

confidence: 99%

Cellulase: Distribution, Production, Characterization and Industrial Applications

Maravi

Kumar

2021

BJI

View full text Add to dashboard Cite

Cellulase enzyme complex is comprised of three enzymes namely exo-glucanase, endo-glucanase and β-glucosidase which act synergistically to deconstruct cellulosic biomass in order to produce fermentable sugars. The enzymes are produced naturally by the living organisms such as bacteria, fungi and algae. The majority of microorganisms that live in extreme environments including hot/cold springs, rumen stomach, deep ocean trench, acidic/alkaline pH environment, have been regarded as appealing producers of cellulase. Cellulases produced by microorganisms have enormous applications in different industries such as agriculture, food and feed production, brewing, textile, laundry and biofuel production. Scientists as well as industry researchers consider cellulases as a prospective candidate for further studies due to the intricacy of the enzyme system and massive industrial potential. Scientific belief in its production and further studies challenges are receiving greater attention these days, notably in the intent of decreasing its production cost at the industrial scale. In this review, future possibilities of using cellulase for various industrial applications are also addressed.

show abstract

Section: Cellulase In Waste Managementmentioning

confidence: 99%

Cellulase: Distribution, Production, Characterization and Industrial Applications

Maravi

Kumar

2021

BJI

View full text Add to dashboard Cite

show abstract

“…Agricultural wastes such as rice straw, wheat straw, sorghum straw, sugarcane bagasse, corncob, sawdust, peanut shell, banana peel, orange peel, pomegranate peel, and water hyacinth are some examples of low‐cost feedstock that may be used to make cellulase by traditional techniques. However, these conventional methods might result in severe environmental contamination, further damaging human and animal health [10]. Hence, the use of various cellulolytic microbes and their cellulase enzymes for the eco‐friendly bioconversion of cellulose into useful compounds, like alcohol and sugar, has attracted attention on a global scale [8].…”

Section: Introductionmentioning

confidence: 99%

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

2023

View full text Add to dashboard Cite

Among 24 isolated cellulolytic bacteria from Similipal Biosphere Reserve, the most efficient isolate was recognized as a strain of Bacillus albus. This strain of B. albus was evaluated for cellulase production and the cellulase activity was measured in submerged fermentation using substrate carboxymethyl cellulose (CMC). Different nutritional (carbon, nitrogen, and metal‐ion sources) and physical variables (pH, temperature, substrate concentration, and incubation time) during the growth of B. albus were optimized to obtain maximum cellulase activity. The highest cellulase activity of 5.79 U/mL for B. albus was observed at pH 6.75, temperature 37.5°C, CMC concentration 8.5 g/L, and 42 h incubation time. Further, supplementation of glucose as a subsidiary carbon source, yeast extract, peptone as nitrogen sources, and MgSO4 and MnSO4 as metal‐ion sources enhance the cellulase activity of B. albus. The purified enzyme was reported to have a molecular weight of ∼54 kDa as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. A zymogram analysis evidenced the cellulase activity of the purified enzyme fractions obtained from diethylaminoethyl cellulose chromatography. The purified cellulase was reported to have an optimum pH and temperature of 7.0°C and 50°C, respectively with a capacity of retaining its 60% residual activity within pH 6.0–8.0 and temperature 30–40°C, respectively. The metal ions, K+ and Na+ were the activators, while Pb2+ and Hg2+ were the inhibitors for the purified cellulase. The purified cellulase showed Km and Vmax values of 0.38 M and 8.19 U/mL, respectively, in presence of the substrate CMC and also simultaneous consumption of both hexose and pentose sugars.

show abstract